GENERAL SCIENTIFIC

一般科学

基本信息

批准号：
8376066
负责人：
Helen M Bramlett
金额：
$ 23.55万
依托单位：
UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8376066
关键词：
Anesthesia procedures Animal Model Animals Antibodies Area Biochemical Biological Assay Brain Brain Injuries Catheterization Cell Count Cells Contusions Core Facility Dissection Electrons Electrophysiology (science)Equipment Excision Faculty Freezing Frequencies Future Harvest Human Resources Image Analysis Imagery Individual Infusion procedures Injury Intubation Investigation Light Liquid substance Methods Microscopic Microtomy Monitor Neurons Operative Surgical Procedures Percussion Perfusion Pharmaceutical Preparations Physiological Plastic Embedding Preparation Procedures Production Protocols documentation Recovery Regulation Research Personnel Sampling Second Messenger Systems Staining method Stains Suspension substance Suspensions Temperature Tracheotomy procedure Transmission Electron Microscopy Transplantation Traumatic Brain Injury Viral Vector brain tissue computerized immunocytochemistry programs rectal sample fixation second messenger tissue processing

项目摘要

The purpose of the General Scientific Core B is to assist investigators in animal surgery, electrophysiology, immunocytochemistry, transmission electron microscopy, and image analysis requirements for the Program Project faculty. The Core also assists investigators in various biochemical analyses and viral vector production. The Core facilities consist of personnel and equipment needed to perform the proposed studies in Projects 1 through 3. This Core will conduct general animal preparation, including anesthesia, intubation, catheterization, temperature monitoring, and regulation of physiological variables. The Core will assist investigators in preparing the animals for traumatic brain injury, as described in the individual projects. In addition, expertise is available to modify experimental animal preparations that may be required for future investigations. Core personnel will perform perfusion, fixation, and removal of brains for light and electron microscopic examination. Core personnel will carry out immunocytochemical procedures, as well as develop new procedures for the visualization of additional antibodies. The Core will process tissue for plastic embedding for both light and electron microscopic analysis. High quality thin sections will be produced by personnel within this Core and stained for ultrastructural analysis. Established image analysis procedures using non-biased stereological procedures to quantify the frequency of immunoreactive cells, as well as to determine contusion areas and volumes will be utilized.

通用科学核心 B 的目的是协助动物外科、电生理学、该计划的免疫细胞化学、透射电子显微镜和图像分析要求项目师资队伍。核心还协助研究人员进行各种生化分析和病毒载体生产。核心设施包括进行拟议研究所需的人员和设备在项目 1 至 3 中。该核心将进行一般动物准备，包括麻醉、插管、导管插入术、温度监测和生理变量的调节。核心将协助研究人员为动物做好创伤性脑损伤的准备，如各个项目中所述。在此外，还可以利用专业知识来修改未来可能需要的实验动物制剂调查。核心人员将进行光电子灌注、固定和摘除大脑显微镜检查。核心人员将进行免疫细胞化学程序，并开发额外抗体可视化的新程序。核心将加工塑料组织用于光学和电子显微镜分析的嵌入。高质量的薄片将由该核心内的人员并进行染色以进行超微结构分析。建立图像分析程序使用无偏见的立体学程序来量化免疫反应细胞的频率，以及确定要使用的挫伤面积和体积。