Laboratory

实验室

• 批准号: 8376492
• 负责人: HUA SU
• 金额: $ 22.74万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8376492
• 关键词: Animal Model; Animals; Blood Vessels; Brain; Brain Vascular Malformation; Cerebrum; Clinical; Coupled; Development; Dysplasia; Genetic; Growth Factor; Homeobox Genes; Human; Image Analysis; Infusion procedures; Integrins; Laboratories; Matrix Metalloproteinases; Mediating; Methodology; Modeling; Molecular and Cellular Biology; Mus; Operative Surgical Procedures; Paraffin; Quality Control; Research Infrastructure; Rodent; Rodent Model; Specimen; Tissue Banking; Tissue Banks; Transgenic Animals; Transplantation; Vascular Endothelial Growth Factors; Vascular remodeling; Vasodilator Agents; Writing; animal breeding; arterial remodeling; design; hemodynamics; in vivo; novel; programs; research study; tool

Integrative Study of Brain Vascular Malformations The Laboratory Core (Core C) will provide infrastructure and experimental methodology for the program. Core C will function as a small animal surgical lab, a cellular and molecular biology lab, an imaging analysis lab, and a transgenic animal breeding unit. Key portions of the in vivo experiments within the PPG will be performed in the Core, thus providing quality control and consistency for the experiments. Our Specific Aims are: (Aim 1) Provide several animal models to the laboratory projects, including reproducible vascular dysplasia models, a flow-induced adaptive outward arterial remodeling model, and human-to-mouse transplantation model in the mature rodent brain (Projects 2, 3 and 4); (Aim 2) Continue to refine our current models and develop novel animal models using manipulation of growth factor stimulation, genetic background and hemodynamic manipulation; (Aim 3) Facilitate transfer of human specimens and serve as the clinical tissue bank for human surgical specimens. Core C will assist in designing the experiments, writing experimental proposals, and solving methodological problems; it will also provide a forum for discussing and interpreting results.

脑血管畸形的综合研究 实验室核心（核心C）将为该计划提供基础架构和实验方法。 核心C将充当小动物外科实验室，一个细胞和分子生物学实验室，成像 分析实验室和转基因动物育种单元。 PPG中体内实验的关键部分 将在核心中进行，从而为实验提供质量控制和一致性。 我们的具体目的是：（目标1）为实验室项目提供多种动物模型，包括 可再现的血管发育不良模型，一种流动诱导的自适应外向动脉重塑模型，并且 成熟的啮齿动物大脑中的人向小鼠移植模型（项目2、3和4）； （目标2）继续 通过操纵生长因子来完善我们当前的模型并开发新型动物模型 刺激，遗传背景和血液动力学操纵； （目标3）促进人类的转移 标本并用作人类手术标本的临床组织库。 核心C将有助于设计实验，编写实验建议并解决方法论学 问题；它还将提供一个讨论和解释结果的论坛。