In vivo studies of neural system in zebrafish

斑马鱼神经系统的体内研究

• 批准号: 8559271
• 负责人: Fumihito Ono
• 金额: $ 80.52万
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8559271
• 关键词: Afferent Neurons; Axon; Behavior; Biochemical Pharmacology; Biological Models; Calcium Channel; Canada; China; Cholinergic Receptors; Development; Differentiation and Growth; District of Columbia; Family member; Fishes; G-Protein-Coupled Receptors; Gene Family; Genes; Genetic; Genetic Screening; Golgi Apparatus; Growth and Development function; International; Interneurons; Introns; Ion Channel; Journals; Kidney; Massachusetts; Midbrain structure; Modification; Muscle; Nature; Nervous System Physiology; Nervous system structure; Neurology; Neuromuscular Junction; Neurosciences; Optics; Otic Vesicle; Paper; Pennsylvania; Physiology; Play; Publishing; Role; Skeletal Muscle; Societies; Sodium Channel; Spinal; Spinal Cord; Stem cells; System; Techniques; Tissues; Transgenic Organisms; Universities; Vertebrates; Zebrafish; alcohol response; comparative; hindbrain; in vivo; meetings; mutant; neurophysiology; posters; receptor; red fluorescent protein; relating to nervous system; symposium; synaptogenesis; zebrafish development

In 2011-2012, we published eight papers. Among them, two were focused on the neuromuscular junctions. In Mongeon et al. published in the Journal of General Physiology, we revealed the differential composition of nicotinic ACh receptors (AChR) in two kinds of skeletal muscles, slow muscle and fast muscle. In Park et al. published in the Journal of Neuroscience, we discovered that AChRs, which are classically considered a passive player in the synapse formation, plays an active role, by allowing the exit of raspy molecule from the Golgi complex. In Papke at al. published in Biochemical Pharmacology, we examined the function of nicotinic AChRs in zebrafish, including muscle type and neural type. We compared the pharmacological profile of zebrafish receptors to those of mammalian receptors. Three papers were published resulting from the forward genetics screening performed in the lab. A gene trap line with an insertion of Red Fluorescent Protein (RFP) in the first intron of Pax8 gene led to the expression of RFP in multiple tissues, including otic vesicles, midbrain-hindbrain boundary, spinal cord and kidney. The insertion also caused the inhibition of Pax8 expression. In Ikenaga et al. published in the Journal of Comparative Neurology, the role of Pax8 and its gene family member Pax2 was examined in the differentiation of spinal interneurons. In Nakayama et al. published in Development Growth and Differentiation, we combined the Pax8-RFP fish with another transgenic line, and examined the axon development of Pax8 expressing interneurons across the midline. In Diep et al. published in Nature, the expression of RFP in kidney was used to analyze the development of renal progenitor cells. In two papers, Won et al. published in the Journal of Neurophysiology and Won et al. published in PLoS ONE, we analyzed sodium and calcium channels expressed in primary sensory neurons, and studied their modifications by G-protein coupled receptors. We presented posters in several meetings including the Society for Neuroscience Meeting (Washington DC) and the International Conference on Zebrafish Development and Genetics (Madison, WI). Fumi Ono gave several invited talks at Universities (University of Massachusetts, Pennsylvania State University) and at international meetings (Neurotalk2012, Beijing, China; Ion Channel retreat, Vancouver, Canada; the International Conference on Zebrafish Development and Genetics, Madison, WI).

2011-2012年，我们发表论文8篇。其中，有两个重点关注神经肌肉接头。在蒙吉恩等人。发表在《普通生理学杂志》上，我们揭示了两种骨骼肌（慢肌和快肌）中烟碱型乙酰胆碱受体（AChR）的差异组成。在帕克等人。发表在《神经科学杂志》上的文章中，我们发现乙酰胆碱受体（AChR）在传统上被认为是突触形成中的被动参与者，但它通过允许刺耳的分子从高尔基复合体中退出而发挥着积极的作用。在 Papke 等人。发表在《生化药理学》上，我们研究了斑马鱼烟碱型乙酰胆碱受体的功能，包括肌肉型和神经型。我们将斑马鱼受体的药理学特征与哺乳动物受体的药理学特征进行了比较。实验室进行的正向遗传学筛查发表了三篇论文。在 Pax8 基因的第一个内含子中插入红色荧光蛋白 (RFP) 的基因捕获系导致 RFP 在多个组织中表达，包括耳囊、中脑-后脑边界、脊髓和肾脏。该插入还导致 Pax8 表达的抑制。 在池永等人中。发表在《比较神经学杂志》上的论文研究了 Pax8 及其基因家族成员 Pax2 在脊髓中间神经元分化中的作用。在中山等人。我们将 Pax8-RFP 鱼与另一个转基因品系结合起来，并检查了中线表达 Pax8 的中间神经元的轴突发育。在迪普等人中。发表在《Nature》杂志上，利用肾脏中 RFP 的表达来分析肾祖细胞的发育。在两篇论文中，Won 等人。发表在《神经生理学杂志》和 Won 等人上。我们分析了初级感觉神经元中表达的钠和钙通道，并研究了 G 蛋白偶联受体对它们的修饰，该研究发表在 PLoS ONE 上。 我们在几次会议上展示了海报，包括神经科学学会会议（华盛顿特区）和国际斑马鱼发育和遗传学会议（威斯康星州麦迪逊）。 Fumi Ono 在大学（马萨诸塞大学、宾夕法尼亚州立大学）和国际会议（Neurotalk2012，中国北京；离子通道静修会，加拿大温哥华；斑马鱼发育和遗传学国际会议，威斯康星州麦迪逊）上进行了多次受邀演讲。