In vivo studies of neural system in zebrafish

斑马鱼神经系统的体内研究

• 批准号: 9155448
• 负责人: Fumihito Ono
• 金额: $ 19.09万
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9155448
• 关键词: Acetylcholine; American Association for the Advancement of Science; Award; Behavior; Behavioral; Biological Models; Chairperson; Chemical Synapse; Chemicals; Cholinergic Receptors; Fellowship; Gene Pool; Genetic; Japan; Journals; Lead; Left; Maryland; Modeling; Mutagenesis; Mutation; National Institute on Alcohol Abuse and Alcoholism; Nervous System Physiology; Nervous system structure; Neuromuscular Junction; Neurotransmitters; Optics; Paper; Pharmacologic Substance; Phenotype; Physiological; Physiology; Positioning Attribute; Postdoctoral Fellow; Proteins; Publishing; Reporting; Resources; Science; Science Policy; Signal Transduction; South Korea; Swimming; Synapses; System; Techniques; Transgenic Organisms; Universities; Vertebrates; Zebrafish; alcohol response; in vivo; lecturer; medical schools; mutant; professor; relating to nervous system; research study; screening

This report covers a period of 6 months, up to 03/2015, when the Section of Model Synaptic Systems closed. The section chief, Dr. Fumihito Ono, left NIAAA on 03/27/2015 to become Professor and Chairman at the Department of Physiology, Osaka Medical College in Japan. Postdocs in the section also moved to their next position during this period. Dr. Tory Williams accepted the position of lecturer at the University of Maryland. Dr. Meghan Mott was awarded a fellowship for science policy at American Association for the Advancement of Science (AAAS). Dr. Jee-Young Park was hired by a pharmaceutical company in South Korea. A review paper entitled Zebrafish mutants of the neuromuscular junction: Swimming in the gene pool was published in the Journal of Physiological Sciences. It provides an overview of zebrafish mutants with dysfunctional acetylcholine receptors or related proteins at the neuromuscular junction (NMJ). The NMJ, which has served as the classical model of the chemical synapse, uses acetylcholine as the neurotransmitter, and mutations of proteins involved in the signaling cascade lead to a variety of behavioral phenotypes. Mutants isolated after random chemical mutagenesis screening were summarized, and advances in the field resulting from these mutants were discussed in the review. In order to complete projects started at NIAAA, resources including established genetic lines were transferred to a new lab in Osaka. The results obtained from these projects will be published as soon as remaining experiments are completed.

本报告涵盖 6 个月的时间，截至 2015 年 3 月，模型突触系统部分关闭。科长 Fumihito Ono 博士于 2015 年 3 月 27 日离开 NIAAA，成为日本大阪医学院生理学系教授兼系主任。在此期间，该部门的博士后也转移到了下一个职位。托里·威廉姆斯博士接受了马里兰大学讲师的职位。梅根·莫特博士荣获美国科学促进会 (AAAS) 科学政策奖学金。 Jee-Young Park 博士受聘于韩国一家制药公司。 题为《斑马鱼神经肌肉接头突变体：在基因库中游泳》的综述论文发表在《生理科学杂志》上。它概述了神经肌肉接头 (NMJ) 乙酰胆碱受体或相关蛋白功能失调的斑马鱼突变体。 NMJ 作为化学突触的经典模型，使用乙酰胆碱作为神经递质，信号级联中涉及的蛋白质突变导致多种行为表型。总结了随机化学诱变筛选后分离出的突变体，并在综述中讨论了这些突变体在该领域取得的进展。 为了完成 NIAAA 启动的项目，包括已建立的遗传系在内的资源被转移到大阪的一个新实验室。这些项目获得的结果将在剩余实验完成后立即发布。

项目成果

Big answers from a small fly with a cultured brain.

一只拥有发达大脑的小苍蝇给出了伟大的答案。

• DOI: 10.1113/jphysiol.2012.246538
• 发表时间: 2013
• 期刊: The Journal of physiology
• 作者: Ono,Fumihito

Zebrafish model for congenital myasthenic syndrome reveals mechanisms causal to developmental recovery.

先天性肌无力综合征的斑马鱼模型揭示了发育恢复的机制。

• DOI: 10.1073/pnas.1215858109
• 发表时间: 2012
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Walogorsky,Michael;Mongeon,Rebecca;Wen,Hua;Nelson,NathanR;Urban,JasonM;Ono,Fumihito;Mandel,Gail;Brehm,Paul
• 通讯作者: Brehm,Paul

A single mutation in the acetylcholine receptor δ-subunit causes distinct effects in two types of neuromuscular synapses.

乙酰胆碱受体 δ 亚基的单个突变会对两种类型的神经肌肉突触产生不同的影响。

• DOI: 10.1523/jneurosci.0426-14.2014
• 发表时间: 2014
• 期刊: The Journal of neuroscience : the official journal of the Society for Neuroscience
• 作者: Park,Jee-Young;Mott,Meghan;Williams,Tory;Ikeda,Hiromi;Wen,Hua;Linhoff,Michael;Ono,Fumihito
• 通讯作者: Ono,Fumihito

Acetylcholine receptors enable the transport of rapsyn from the Golgi complex to the plasma membrane.

乙酰胆碱受体能够将rapsyn从高尔基复合体转运到质膜。

• DOI: 10.1523/jneurosci.0397-12.2012
• 发表时间: 2012
• 期刊: The Journal of neuroscience : the official journal of the Society for Neuroscience
• 作者: Park,Jee-Young;Ikeda,Hiromi;Ikenaga,Takanori;Ono,Fumihito
• 通讯作者: Ono,Fumihito