HIGH RESOLUTION STRUCTURES BY MINIMIZING RADIATION EFFECTS

通过最小化辐射效应实现高分辨率结构

• 批准号: 7721228
• 负责人: JUN WANG
• 金额: $ 0.1万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-15 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721228
• 关键词: Affect; Attention; Computer Retrieval of Information on Scientific Projects Database; Data; Data Set; Funding; Generations; Grant; Institution; Proteins; Radiation; Research; Research Personnel; Resolution; Resources; Source; Standards of Weights and Measures; Structure; Synchrotrons; Time; United States National Institutes of Health; radiation effect; ultra high resolution

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Radiation damage on protein crystals, especially since data is collected at third generation synchrotron facilities, is an issue and is getting more and more attention. Ultra high resolution structures are what people aspire to obtain. However, ultra high resolution structures are most sensitive to and affected by radiation damage. We applied the new strategy we developed for time-dependent structure observations to remove or minimize radiation effects and thus yield the real high resolution structure from a standard dataset.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 蛋白质晶体的辐射损伤是一个问题，特别是因为数据是在第三代同步加速器设施中收集的，并且受到越来越多的关注。 超高分辨率结构是人们渴望获得的。 然而，超高分辨率结构对辐射损伤最为敏感并受其影响。 我们应用了为时间相关结构观测开发的新策略，以消除或最小化辐射效应，从而从标准数据集中产生真正的高分辨率结构。