SPECIFIC CHEMICAL INTERACTIONS IN MATRIX-INCORPORATED INSULIN STRUCTURES

基质结合的胰岛素结构中的特定化学相互作用

• 批准号: 7721229
• 负责人: JUN WANG
• 金额: $ 0.1万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-15 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721229
• 关键词: Cations; Chemicals; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Institution; Insulin; Methods; Motivation; Play; Proteins; Protons; Research; Research Personnel; Resources; Role; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Structure; Techniques; United States National Institutes of Health; Work; macromolecule

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been a powerful technique in the bio-macromolecules field. The matrix, a small organic molecule, plays a key role in the mass spectral method. The MALDI method has been developed empirically and its mechanism is not yet fully understood. The motivation of this project is to understand how the matrix molecule works or how it interacts with the protein. The aim of this work is to find specific interactions between the protein and the matrix molecule, so it will help explain the formation of a protein cation and how the proton transfer occurs.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 基质辅助激光解吸/电离质谱（MALDI-MS）已成为生物大分子领域的一项强大技术。 基质是一种有机小分子，在质谱方法中起着关键作用。 MALDI 方法是凭经验开发的，其机制尚未完全了解。 该项目的动机是了解基质分子如何工作或它如何与蛋白质相互作用。 这项工作的目的是找到蛋白质和基质分子之间的特定相互作用，因此它将有助于解释蛋白质阳离子的形成以及质子转移如何发生。