Ophthalmic Imaging Using Adaptive Optics and OCT

使用自适应光学和 OCT 进行眼科成像

基本信息

批准号：
8539869
负责人：
JOHN S WERNER
金额：
$ 7.59万
依托单位：
UNIVERSITY OF CALIFORNIA AT DAVIS
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-09-30 至 2013-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8539869
关键词：
Affect Age Age related macular degeneration American Astronomy Birefringence Blindness California Cell Volumes Cells Cellular Structures Clinical Companions Complex Computer software Data Data Set Detection Dimensions Disease Drusen Early Diagnosis Engineering Excision Eye Fiber Fluorescence Functional Imaging Functional disorder Ganglia Glaucoma Goals Health Hemoglobin Hereditary Retinal Dystrophy Histology Human Image Imagery Imaging Techniques Indiana Individual Institution Laboratories Lateral Lead Life Light Location Macular degeneration Maps Measurement Measures Methods Molecular Morphologic artifacts Motion Nerve Fibers Neurons Optic Nerve Optical Coherence Tomography Optics Oxygen Patients Photoreceptors Process Property Research Research Personnel Resolution Retina Retinal Retinal Cone Retinal Degeneration Retinal Vein Occlusion Risk Scanning Scientist Site Source Speed Structure Structure of retinal pigment epithelium Syncope System Techniques Technology Testing Three-Dimensional Imaging Universities Vision Vision Disorders Visual adaptive optics base blood perfusion cell type implantable device improved in vivo instrument instrumentation interest nanoscale new technology normal aging novel optic nerve disorder programs retinal neuron retinal rods sample fixation simulation success tool virtual vision science

项目摘要

DESCRIPTION (provided by applicant): The purpose of this BRP is to develop and evaluate technology for three-dimensional imaging of cells in the living eye, and to use this novel technology to understand changes in cell layers associated with the most common diseases leading to world-wide blindness, including age-related macular degeneration and glaucoma. Partners at four institutions will contribute to instrumentation that combines adaptive optics (AO), providing high lateral resolution, with optical coherence tomography (OCT), providing high axial resolution. The lead institution is the University of California, Davis (John S. Werner, PI). Both at UC Davis and at Indiana University (Donald T. Miller, site PI), AO-OCT instrumentation will be developed and tested. Duke University (Joseph Izatt, site PI) will develop novel OCT approaches that will be incorporated, while Lawrence Livermore National Laboratory (Scot Olivier, site PI) will provide cutting-edge advances in AO. These AO-OCT instruments will permit human in vivo imaging with sufficient resolution and contrast to visualize the smallest of cells in the human retina. In the previous project period we have used AO-OCT to create volume images of structures previously only visible with histology, including the photoreceptor outer segments, Fibers of Henle, individual optic nerve fiber bundles, detailed structures within drusen of macular degeneration patients, and fine structure of the lamina cribosa of the optic nerve. In this renewal, we propose to solve new technical issues to more fully tap the potential and functionality of AO-OCT. The engineering goals are directed toward increasing contrast of cellular structures by use of autofluoresence, birefringence, the incorporation of "extreme AO" techniques, and functional changes associated with blood perfusion, hemoglobin oxygen saturation in the retinal vasculature as well as electrical changes in neuronal cell volumes. Considerable effort will be devoted to three-dimensional visualization and segmentation over retinal volumes of larger lateral and axial extent than previously possible. The engineering goals have parallel clinical aims for improving our understanding of the cellular changes associated with markers for age-related macular degeneration and glaucoma. These advanced imaging techniques will be deployed for evaluating the changes in the retina and optic nerve resulting from novel therapies for rescuing retinal layers from the leading causes of blindness.

描述（由申请人提供）：该BRP的目的是开发和评估生命中细胞三维成像的技术，并使用这种新型技术来了解与导致全球失明的最常见疾病相关的细胞层的变化，包括与年龄相关的黄斑变性和青光眼。四个机构的合作伙伴将有助于结合自适应光学元件（AO），提供高横向分辨率，以及光学连贯性断层扫描（OCT），提供高轴向分辨率。主要机构是加利福尼亚大学戴维斯分校（John S. Werner，PI）。在加州大学戴维斯分校和印第安纳大学（Donald T. Miller，Site PI），AO-O-OCT仪器都将得到开发和测试。杜克大学（Joseph Izatt，Site PI）将开发新颖的OCT方法，而劳伦斯·利弗莫尔国家实验室（Scot Olivier，Site PI）将在AO中提供最先进的进步。这些AO-O-OCT仪器将允许人体成像具有足够的分辨率，并形成对比的人类视网膜中最小的细胞。在上一个项目期间，我们已经使用AO-OCT创建了以前仅具有组织学可见的结构的体积图像，包括光感受器的外部细分，Henle的纤维，单个视神经纤维束，黄斑变性患者的详细结构以及光学结构的细微结构。在此续约中，我们建议解决新的技术问题，以更充分地利用AO-OCT的潜力和功能。工程目标是通过使用自动荧光，双发性，“极端AO”技术的结合以及与血液灌注相关的功能变化，视网膜脉管体中的血红蛋白氧饱和度相关的功能变化以及神经元细胞量的电气变化。比以前可能更大的视网膜体积，将大量的努力用于三维可视化和分割。工程目标具有平行的临床目的，目的是提高我们对与年龄相关的黄斑变性和青光眼相关的细胞变化的理解。这些先进的成像技术将被部署，以评估因新颖的疗法而导致的视网膜和视神经的变化，用于从盲目的主要原因中拯救视网膜层。