Structure and Function of the Chorioretinal Complex in Age-Related Macular Degene

年龄相关性黄斑变性中脉络膜视网膜复合体的结构和功能

• 批准号: 9034586
• 负责人: JOHN S WERNER
• 金额: $ 47.2万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-01 至 2019-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9034586
• 关键词: Address; Affect; Age related macular degeneration; Algorithms; Angiography; Area; Atrophic; Biological Aging; Blindness; Blood Vessels; Blood flow; Bruch' s basal membrane structure; Cell physiology; Characteristics; Choroid; Choroidal Neovascularization; Complex; Cone; Dependence; Detection; Development; Disease; Drusen; Early Diagnosis; Evaluation; Exudative age-related macular degeneration; Eye; Eye diseases; Fluorescein; Functional disorder; Fundus; Future; Generations; Goals; Health; Hour; Human; Image; Imaging technology; Indocyanine Green; Injection of therapeutic agent; Knowledge; Lasers; Lateral; Length; Life; Light; Localized Disease; Location; Maps; Measures; Methods; Microscopic; Modality; Morphology; Nonexudative age-related macular degeneration; Ophthalmoscopy; Optical Coherence Tomography; Optics; Pathogenesis; Patients; Penetration; Perfusion; Phagocytosis; Phase; Photoreceptors; Psychophysics; Research; Resolution; Retina; Retinal; Retinal Cone; Retinal Diseases; Retrieval; Rhodopsin; Scanning; Scheme; Secondary to; Source; Speed; Staging; Structure; Structure of retinal pigment epithelium; System; Technology; Testing; Thick; Time; Ursidae Family; Vascular Endothelial Growth Factors; Vascularization; Work; adaptive optics; age related; base; density; effective therapy; geographic atrophy; human disease; image processing; in vivo; in vivo imaging; indexing; inhibitor/antagonist; macula; novel; optical imaging; phase change; programs; retinal rods; screening; therapy design; tool; Address; Affect; Age related macular degeneration; Algorithms; Angiography; Area; Atrophic; Biological Aging; Blindness; Blood Vessels; Blood flow; Bruch' s basal membrane structure; Cell physiology; Characteristics; Choroid; Choroidal Neovascularization; Complex; Cone; Dependence; Detection; Development; Disease; Drusen; Early Diagnosis; Evaluation; Exudative age-related macular degeneration; Eye; Eye diseases; Fluorescein; Functional disorder; Fundus; Future; Generations; Goals; Health; Hour; Human; Image; Imaging technology; Indocyanine Green; Injection of therapeutic agent; Knowledge; Lasers; Lateral; Length; Life; Light; Localized Disease; Location; Maps; Measures; Methods; Microscopic; Modality; Morphology; Nonexudative age-related macular degeneration; Ophthalmoscopy; Optical Coherence Tomography; Optics; Pathogenesis; Patients; Penetration; Perfusion; Phagocytosis; Phase; Photoreceptors; Psychophysics; Research; Resolution; Retina; Retinal; Retinal Cone; Retinal Diseases; Retrieval; Rhodopsin; Scanning; Scheme; Secondary to; Source; Speed; Staging; Structure; Structure of retinal pigment epithelium; System; Technology; Testing; Thick; Time; Ursidae Family; Vascular Endothelial Growth Factors; Vascularization; Work; adaptive optics; age related; base; density; effective therapy; geographic atrophy; human disease; image processing; in vivo; in vivo imaging; indexing; inhibitor/antagonist; macula; novel; optical imaging; phase change; programs; retinal rods; screening; therapy design; tool

DESCRIPTION (provided by applicant): Three aims are proposed to study the chorioretinal complex (choroid, choriocapillaris, Bruch's membrane, retinal pigment epithelium, photoreceptors) in age-related macular degeneration (AMD). Each aim includes both technical goals involving optical imaging and tests of hypotheses related to the pathogenesis of this disease. Aim 1 will use phase changes between successive optical coherence tomography (OCT) B-scans (frames) to visualize the vascular layers behind the retina, specifically the choriocapillaris, Sattler's and Haller's layers of the choroid. Use of a 1050 nm light source will provide deep penetration through the retinal pigment epithelium to study changes in vascularization associated with nonexudative AMD, a condition for which there is currently no effective treatment. It will also permit in vivo examination of changes in subretinal vascularization associated with anti-VEGF treatment for neovascular AMD, a treatment that is generally effective but not completely understood. Aim 2 will measure fundus autofluorescence (FAF) and morphology of the retinal pigment epithelium in geographic atrophy using ultrahigh-resolution adaptive optics (AO) with simultaneous reflectance and fluorescent scanning laser ophthalmoscopy and OCT volumetric imaging to co- localize disease-related changes visualized with the two modalities. This aim will investigate the hypothesis that some of the changes in FAF characterizing AMD are secondary to changes in rhodopsin photopigment screening of the excitation and emitted light. This result may alter interpretations of FAF changes in AMD and other diseases of the chorioretinal complex associated with rod photoreceptor losses, and will be critical in developing or assessing new treatments. Aim 3 will use a newly constructed afocal AO-OCT system and phase retrieval algorithm to measure changes in length and renewal rates of the photoreceptor outer segments in normal retinae and those with drusen characteristic of early and intermediate stage AMD. Changes in photoreceptors will be characterized over short- and long-term time scales. These results may provide new and sensitive functional indicators of AMD progression and a leading indicator of changes in the health of the chorioretinal complex at the intersection between biological aging and eye disease.

描述（由申请人提供）：提出了三个目的，以研究与年龄相关的黄斑变性（AMD），研究脉络膜结构络合物（脉络膜，脉络膜毛细血管，Bruch的膜，视网膜色素上皮，感光体）。每个目标都包括涉及光学成像的技术目标和与该疾病发病机理有关的假设的检验。 AIM 1将使用连续的光学相干断层扫描（OCT）B扫描（框架）之间的相变，以可视化视网膜后面的血管层，特别是脉络膜胶质，Sattler's和Haller的脉络膜层。使用1050 nm的光源将通过视网膜色素上皮提供深层渗透，以研究与非xudational AMD相关的血管形成变化，目前尚无有效治疗的情况。它还将允许体内检查与抗VEGF治疗新血管AMD相关的视网膜下血管的变化，这种治疗方法通常是有效但尚未完全理解的。 AIM 2将使用超高分辨率自适应光学（AO）测量地理萎缩中视网膜色素上皮的眼底自动荧光（FAF）和形态，具有同时反射率和荧光扫描激光扫描激光眼镜检查，并具有两种模式化的与疾病相关的可视化率，并与本地化疾病相关的变化。该目的将调查以下假设：FAF表征AMD的某些变化是励磁和发光的变化筛查的变化。该结果可能会改变对AMD的FAF变化的解释以及与Rod感光者损失相关的脉络膜结构复合物的其他疾病，并且对于开发或评估新疗法至关重要。 AIM 3将使用新建造的Adafocal AO-OCT系统和相检索算法来测量正常视网膜中光感受器外部段的长度变化和更新速率，以及具有早期和中等阶段AMD的DRUSEN特征的变化。光感受器的变化将在短期和长期尺度上进行表征。这些结果可能提供了AMD进展的新的和敏感的功能指标，并且是生物衰老与眼部疾病之间交集的脉络膜内复合物健康变化的主要指标。