Atypical opsins and the OIR model of retinopathy of prematurity

非典型视蛋白与早产儿视网膜病变的 OIR 模型

基本信息

批准号：
10675898
负责人：
JEFFREY H BOATRIGHT
金额：
$ 23.48万
依托单位：
EMORY UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-06-01 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10675898
关键词：
Affect Age related macular degeneration Air Blindness Blood Vessels Blood capillaries Caring Cells Clinical Contrast Sensitivity Data Detection Development Diabetic Retinopathy Disease Dopamine Exposure to Eye Eye Development Female Gene Deletion Histology Homeostasis Incubators Infant KDR gene Knock-out Knockout Mice Light Lighting Modeling Mus Mutant Strains Mice Nature Neonatal Neurons Opsin Outcome Measure Oxygen Oxygen Therapy Care Pathologic Neovascularization Pathway interactions Perinatal Phototherapy Physicians Predisposition Pregnancy Premature Infant Preventive treatment Protocols documentation Research Retina Retinal Diseases Retinal Ganglion Cells Retinal Pigments Retinopathy of Prematurity Risk Role Severities Signal Transduction Testing Traction VEGFA gene Vascular Endothelial Growth Factors Vertebrate Photoreceptors Viola Vision Visual Acuity density experience experimental study innovation male melanopsin mouse model neonatal care neonate neovascularization neurodevelopment perinatal period postnatal prenatal prevent response supplemental oxygen

项目摘要

Abstract Retinopathy of prematurity (ROP) is a retinal disease characterized by aberrant vascular and neural development that affects prematurely born infants receiving supplemental oxygen therapy. Advances in neonatal care enable physicians to save the lives of increasingly premature infants. However, these infants are at great risk for developing ROP because the high oxygen levels in neonatal incubators suppress retinal vascular development. When returned to room air, there is a compensatory pathological neovascularization that results in retinopathy. Retinal ganglion cell opsins, melanopsin (OPN4) and neuropsin (OPN5), regulate vascular development in the perinatal period of mice. OPN4 is a blue light-sensitive opsin, while OPN5 is maximally sensitive to visible violet light. An OPN4-dependent light response pathway has been shown to regulate vascular development in the eye by keeping VEGFA levels in check (OPN4-VEGFA pathway) (Rao et al., Nature 2013; 494:243-6). OPN5 also regulates vascular development in the eye. Opn5 null mice show mildly elevated retinal vascular density and neuron number, and promiscuous hyaloid vessel retraction (Nguyen et al., Nature Cell Biol 2019; 21:420-429). OPN5 modulates vascular development via dopamine-regulated VEGFR2 expression (OPN5-dopamine- VEGFR2 pathway). The proposed research will test the hypothesis that stimulation of the OPN4-VEGFA blue light response pathway will protect retinal vascular development in an oxygen-induced retinopathy (OIR) mouse model of ROP, while stimulation of the OPN5-dopamine-VEGFR2 violet light pathway will exacerbate retinopathy. To test of this innovative hypothesis, we propose two specific aims using the mouse OIR model. In aim 1, we will explore the role of OPN4 manipulation, examining the effects of removing environmental blue light or enhancing blue light on the severity of retinopathy, using both wildtype and Opn4 mutant mice. In aim 2, we will explore the role of OPN5 manipulation, examining the effects of removing environmental violet light or enhancing violet light on the severity of retinopathy, using both wildtype and Opn5 mutant mice. Effects of blue light or violet light therapy will be assessed during oxygen treatment (P7-12) and in the ensuing developmental period (P17-P50). Our findings will have broad implications for prenatal and neonatal eye care, and may lead to the development of an innovative, non-invasive, light-based preventative treatment for ROP.

抽象的早产视网膜病变（ROP）是一种视网膜疾病，其特征是异常血管和神经发育这会影响接受补充氧疗法的过早出生的婴儿。新生儿护理的进步启用医生挽救越来越早的婴儿的生命。但是，这些婴儿有很大的风险由于新生儿孵化器中的高氧水平抑制视网膜血管发育。当返回房间空气时，会有一种补偿性病理新生血管形成导致视网膜病变。视网膜神经节细胞Opsins，黑色素蛋白（OPN4）和Neuropsin（OPN5）调节血管发育小鼠的围产期。 OPN4是一种蓝色光敏的OPSIN，而OPN5对可见紫色最大敏感光。已显示依赖OPN4的光响应途径可以调节眼睛的血管发育通过保持VEGFA水平（OPN4-VEGFA途径）（Rao等，Nature 2013; 494：243-6）。 OPN5也是调节眼睛的血管发育。 OPN5 NULL小鼠表现出轻度升高的视网膜血管密度和神经元数和混杂透明血管回缩（Nguyen等人，自然细胞Biol 2019; 21：420-429）。 OPN5通过多巴胺调节的VEGFR2表达调节血管发育（OPN5-多巴胺 - VEGFR2途径）。拟议的研究将检验以下假设：刺激OPN4-VEGFA蓝色光响应途径将保护氧诱导的视网膜病变（OIR）中的视网膜血管发育 ROP的鼠标模型，而刺激OPN5-多巴胺-VEGFR2紫罗兰色光途径将病变病变。为了检验这种创新的假设，我们提出了使用鼠标的两个特定目标 OIR模型。在AIM 1中，我们将探讨OPN4操纵的作用，研究删除的效果使用WildType和Opn4，环境蓝光或增强视网膜病的严重程度的蓝光突变小鼠。在AIM 2中，我们将探索OPN5操纵的作用，研究删除的效果使用WildType和Opn5 突变小鼠。在氧气治疗（P7-12）和IN中，将评估蓝光或紫罗兰光治疗的影响随之而来的发展期（P17-P50）。我们的发现将对产前和新生儿有广泛的影响眼部护理，并可能导致创新的，非侵入性的基于光的预防治疗对于ROP。