Enhancement of the Integrated Aquatic Biomedical Models Facility Core

增强综合水生生物医学模型设施核心

• 批准号: 8186408
• 负责人: Robyn L Tanguay
• 金额: $ 49.97万
• 依托单位: OREGON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-15 至 2012-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8186408
• 关键词: Animal Model; Animals; Automation; Biomedical Research; Breeding; Carcinogens; Core Facility; Custom; Diet; Direct Costs; Doctor of Philosophy; Emergency Situation; Emergency medical service; Equipment; Fishes; Food Supply; Funding; Grant; Health; Housing; Human; Human Biology; Image; Laboratory Research; Liquid substance; Malnutrition; Modeling; Oregon; Pharmaceutical Preparations; Postdoctoral Fellow; Preparation; Process; Research; Research Personnel; Research Training; Robotics; Safety; Salmo trutta; Sampling; Screening procedure; Services; Site; Specific Pathogen Frees; Students; System; Toxicology; Training; Training Support; United States National Institutes of Health; Universities; Veterinarians; Zebrafish; base; carcinogenesis; cost effective; dosage; environmental chemical; germ free condition; innovative technologies; instrumentation; laboratory facility; nanomaterials; prevent; programs; research study

DESCRIPTION (provided by applicant): We request funds to modernize the electric service, the emergency electrical power, and replace the diet preparation kitchen at the Sinnhuber Aquatic Research Laboratory (SARL) at Oregon State University. The 17,000 sq. ft. SARL was built 46 years ago and provides capabilities found nowhere else in the world to support biomedical research and training in aquatic vertebrate models. Aquatic vertebrate models of human biology are rapidly emerging as a rapid, cost effective, and powerful research platform for biomedical studies. Among its notable research accomplishments, the SARL was essential for completing the two largest ultralow dosage carcinogenesis studies ever conducted in any vertebrate species (~42,000 trout) and helped identify alfatoxin as a major human carcinogen. The Facility has recently received an NIH RC4 Director's grant to further develop new robotics and automation to enable zebrafish to become the first vertebrate model organism capable of high throughput and high-content screening. Other equipment recently acquired for SARL, including automated liquid handling, robotic sample processing and analysis, and high content imaging instrumentation, has greatly added to the capacity for studying the pharmacologic, safety and toxicological effects of nanomaterials, drugs, and other environmental chemicals in zebrafish. SARL has achieved a number of milestones to place it in the forefront of zebrafish research. These include: "the first and only specific pathogen-free zebrafish facility," the first defined diet for zebrafish, "a standardized outbred SPF "wild-type" zebrafish," multiple innovative technologies to automate breeding and zebrafish handling. SARL currently houses 24 researchers plus staff and supports $5.2 million in direct costs of federal research in 2010. The SARL facility also supports the training of many students, post- docs and veterinarians in the use of aquatic models. Two T32 training grants are based upon the unique capabilities of the SARL facility: a new training grant for veterinarians (DVM) to earn the Ph.D., and the 36-year T32 Toxicology Training grant at OSU. The requested funds will enable the completion of a seven-year remodeling program to rebuild a core facility will supporting the largest vertebrate experiments ever conducted. The requested emergency power system and new electrical service is necessary to protect fish health and to prevent disruption of long-term experiments. The diet kitchen remodeling is necessary to protect the food supply for the large numbers of animals maintained in the facility as well as to allow custom-defined diets to be prepared on site. This will add to SARL's unique capabilities to probe the effects of nutritional deficiencies to be investigated in zebrafish.

描述（由申请人提供）：我们要求资金使电力服务，紧急电力现代化，并在俄勒冈州立大学的Sinnhuber水上研究实验室（SARL）替换饮食准备厨房。 17,000平方英尺的SARL是46年前建造的，并提供了世界上没有其他在水生脊椎动物模型中支持生物医学研究和培训的功能。人类生物学的水生脊椎动物模型迅速成为生物医学研究的快速，成本效益和强大的研究平台。在其著名的研究成就中，SARL对于完成在任何脊椎动物物种（〜42,000鳟鱼）进行的两项最大的超低剂量致癌研究至关重要，并有助于将阿尔法毒素识别为主要的人致癌。该设施最近获得了NIH RC4董事的赠款，以进一步开发新的机器人技术和自动化，以使斑马鱼成为第一个能够进行高吞吐量和高含量筛查的脊椎动物模型生物体。最近为SAR获得的其他设备，包括自动液体处理，机器人样品处理和分析以及高内容成像仪器，已大大增加了研究Zebrafish中纳米材料，药物和其他环境化学物质的药理学，安全性和毒理学作用的能力。 SARL已取得了许多里程碑，将其置于斑马鱼研究的最前沿。其中包括：“第一个也是唯一特定的无病原体斑马鱼设施”，第一个定义的斑马鱼饮食，这是一种标准化的SPF SPF“野生型”斑马鱼，“多种创新技术”，可自动化繁殖和斑马鱼的处理。 SARL目前拥有24名研究人员以及员工，并支持2010年联邦研究的520万美元的直接成本。SARL设施还支持对许多学生，邮政和兽医的培训，以使用水生模型。两项T32培训赠款基于SARL设施的独特能力：兽医（DVM）获得博士学位的新培训补助金，以及OSU的36年T32毒理学培训补助金。要求的资金将使完成为期7年的改建计划，以重建核心设施将支持有史以来最大的脊椎动物实验。要求的紧急电源系统和新的电气服务对于保护鱼类健康和防止长期实验的破坏是必要的。饮食厨房的改建对于保护设施中的大量动物的食物供应是必要的，并允许在现场准备自定义的饮食。这将增加SARL独特的能力，以探测斑马鱼中要研究的营养缺乏的影响。