K.C. Donnelly Externship - Promotion of Translational/Transdisciplinary Efforts in Graduate and Post-Doctoral Research - Dasgupta

K.C.

基本信息

批准号：
10381316
负责人：
Robyn L Tanguay
金额：
$ 1.38万
依托单位：
OREGON STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-06-16 至 2022-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10381316
关键词：
Active Learning Adult Area Aromatic Polycyclic Hydrocarbons Aryl Hydrocarbon Receptor Atlases Automobile Driving Behavioral Benzo(a)pyrene Bioinformatics Biology Brain Child Cognitive Complex Development Embryo Environmental Pollution Exposure to Fertilization Modeling Nervous System Physiology Neurotoxins Organ Pattern Postdoctoral Fellow Research Role Signal Recognition Particle Superfund Technology Toxic effect Training Water Work Zebrafish cell type chemical group deep sequencing externship neurodevelopmental effect neurotoxic prenatal exposure programs single cell sequencing superfund site transcriptomics

Active Learning Adult Area Aromatic Polycyclic Hydrocarbons Aryl Hydrocarbon Receptor Atlases Automobile Driving Behavioral Benzo(a)pyrene Bioinformatics Biology Brain Child Cognitive Complex Development Embryo Environmental Pollution Exposure to Fertilization Modeling Nervous System Physiology Neurotoxins Organ Pattern Postdoctoral Fellow Research Role Signal Recognition Particle Superfund Technology Toxic effect Training Water Work Zebrafish cell type chemical group deep sequencing externship neurodevelopmental effect neurotoxic prenatal exposure programs single cell sequencing superfund site transcriptomics

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项目摘要

PROJECT DESCRIPTION (ABSTRACT) Polycyclic aromatic hydrocarbons (PAHs)- a group of environmental contaminants commonly found in Superfund sites- have been well-established as developmental neurotoxicants. Prenatal exposure to PAHs has been associated with decreased cognitive status among children. As a part of the OSU SRP, our lab uses the zebrafish model to study the mechanisms of PAH toxicity, including the role of aryl hydrocarbon receptor. Work from our group has shown that in addition to neurodevelopmental effects, developmental exposures to some PAHs, such as benzo-a-pyrene (BaP), result in cognitive and behavioral deficits in adults; however, the mechanisms are still not clear. The brain is a complex organ with specific areas and cell-types regulating various nervous system functions, including behavioral patterns. Single cell sequencing is an ideal technology that can identify cell type-specific transcriptomic atlas of an organ using deep sequencing and established transcriptomic markers. I will achieve training in this technology from Dr. Bruce Danton and Dr. Britton Goodale at the Dartmouth Superfund Center to study changes in the transcriptomic atlas of zebrafish brain that drives persistent neurotoxic effects of PAHs. Briefly, I will expose zebrafish embryos 4 µM BaP- a concentration that has been shown to induce adult behavioral deficits- from 6 to 120 h post fertilization (hpf), grow them out in clean water, dissect their brains and perform single cell sequencing and bioinformatic analyses through hands-on training in Dartmouth. This project will result in a better understanding of the biology driving PAH- induced long-term behavioral disruptions. Through experiential learning, I will add this state-of-the-art technology to my research portfolio and can apply this to diverse groups of chemicals when I transition into my independent research program.

项目描述（摘要）多环芳族烃（PAHS） - 一组常见的环境污染物超级基金站点已成为发展的神经毒性。产前暴露 PAHS与儿童认知状况的改善有关。作为OSU SRP的一部分，我们的实验室使用斑马鱼模型研究PAH毒性的机制，包括芳基的作用碳氢化合物受体。我们小组的工作表明，除了神经发育效应外，对某些PAH的发育暴露，例如苯甲 - 苯 - 苯乙烯（BAP），导致认知和行为定义成人；但是，机制仍然不清楚。大脑是一个复杂的器官特定区域和细胞类型调节各种神经系统功能，包括行为模式。单细胞测序是一种理想的技术，可以识别特定于细胞类型的转录组使用深度测序和建立的转录组标记的器官地图集。我将获得培训在达特茅斯超级基金中心的Bruce Danton博士和Britton Goodale博士的技术中研究斑马鱼大脑的转录组图集的变化，该图谱驱动持续的神经毒性作用 pahs。简而言之，我将暴露斑马鱼胚胎4 µm bap-浓度已显示为诱导成人行为定义 - 受精后6至120小时（HPF），将它们在清洁的水中生长剖析大脑并通过动手进行单细胞测序和生物信息学分析在达特茅斯的培训。该项目将使人们更好地了解驱动PAH-的生物学诱发的长期行为破坏。通过经验学习，我将添加这个最新的我的研究组合技术技术，可以在我过渡时将其应用于潜水的化学物质群体进入我的独立研究计划。