UMass Mouse Metabolic Phenotyping Center

麻省大学小鼠代谢表型中心

• 批准号: 8193746
• 负责人: JASON K KIM
• 金额: $ 43.25万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-16 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8193746
• 关键词: UMass; Mouse; Metabolic; Phenotyping; Center

DESCRIPTION (provided by applicant): The UMass Mouse Metabolic Phenotyping Center involves a multidisciplinary group of investigators at UMass who perform an array of novel and sophisticated metabolic experiments using state-of-the-art equipment for the purpose of investigating transgenic mice useful for understanding diabetes and its complications. The goal of the UMass MMPC is to provide comprehensive metabolic characterization of transgenic mice using unique and standardized techniques mostly involving, in vivo and physiological setting that are complemented by analytical experiments using serum/tissue samples and measure of cardiovascular complications using non-invasive procedures in mice. The UMass MMPC consists of 3 Phenotyping Cores: 1) Metabolism Core applies hyperinsullnemic-euglycemic clamp to assess insulin sensitivity and glucose metabolism in individual organs, hyperglycemic clamp to assess pancreatic beta-cell function in conscious mice, metabolic cages to assess energy expenditure, activity and food intake, and 1H-MRS to measure fat/lean/water mass in mice, 2) Analytical Core uses Luminex. Meso Scale Discovery, and Cobas Clinical Chemistry Analyzer to provide a high-throughput and multiplexed measurement of serum/tissue levels of hormones, cytokines and metabolites, performs molecular experiments to examine insulin signaling, and conducts histological studies to examine tissues, 3) Cardiovascular Complications Core uses Vevo2100 In Vivo Imaging System to conduct echocardiography and tissue Doppler imaging, and CODA blood pressure device to examine cardiovascular abnormalities associated with diabetes. Mice provided by the users are overseen by the Animal Care Core that processes the receiving, quarantine, and housing of mice. Lastly, financial and administrative operation, research & development program, and website & database are managed by the Administrative Core. Overall, the aim of the UMass MMPC is to become an NIH-funded national center that offers elegant and standardized techniques and scientific expertise using a fee-for-service structure to characterize the metabolic and organ function phenotypes of transgenic mice and provide important insights into the mechanism of diabetes and its complications. PUBLIC HEALTH RELEVANCE: Prevalence of diabetes is increasing at an alarming rate, and by year 2025. One out of 3 Americans is expected to be diabetic. Using established expertise, elegant experimental procedures, and state-of-the-art instruments, our goal is to understand how diabetes develops and to identify a cure to treat obesity and diabetes.

描述（由申请人提供）：UMass小鼠代谢表型涉及UMass的多学科研究人员组，他们使用最先进的设备进行了一系列新颖而复杂的代谢实验，以研究用于研究转基因小鼠的目的，可用于了解糖尿病及其复杂性。 UMASS MMPC的目的是使用独特的和标准化的技术在体内和生理环境中提供转基因小鼠的全面代谢表征，通过使用血清/组织样本进行分析实验，并使用小鼠中的非侵入性手术来补充分析实验。 UMASS MMPC由3个表型核心组成：1）代谢核心应用高囊肿液态夹具评估单个器官中胰岛素敏感性和葡萄糖代谢的胰岛素敏感性和葡萄糖代谢，高血糖钳位，评估胰腺β-纤维培养基在评估意识的β-纤维化质量，以评估能量的跨度和食物的临界，并进行较大的益处和食物，并进行了代谢，活动，活动，活动，活动，活动，活动，活动，活动和食物的效果，活动/食物的效果，活动/食物的效果，活动/食物的效果，活动量和食物的作用。在小鼠中，2）分析核心使用Luminex。 MESO量表发现和COBAS临床化学分析仪，可提供对激素，细胞因子和代谢物的血清/组织水平的高通量和多路复用测量，执行分子实验，以检查胰岛素信号传导，以检查胰岛素信号，并进行组织学研究以检查组织，3）核心核心，3）Cardiascular Instry contrice conteriation consuction consuction consuction consuction consution for Veivo2100 Invivo Invocosigh多普勒成像和CODA血压装置检查与糖尿病有关的心血管异常。用户提供的小鼠由动物护理核心监督，该动物护理核心处理小鼠的接收，隔离和住房。最后，财务和行政运营，研发计划以及网站和数据库由行政核心管理。总体而言，UMass MMPC的目的是成为NIH资助的国家中心，该中心使用费用的服务结构提供优雅，标准化的技术和科学专业知识，以表征转基因小鼠的代谢和器官功能表型，并为糖尿病机制及其并发症的机制提供重要的见解。 公共卫生相关性：糖尿病的患病率正在以惊人的速度增加，到2025年，糖尿病的患病率正在以惊人的速度。使用既定的专业知识，优雅的实验程序和最先进的工具，我们的目标是了解糖尿病如何发展并确定治疗肥胖和糖尿病的方法。