UMass Mouse Metabolic Phenotyping Center

麻省大学小鼠代谢表型中心

基本信息

批准号：
10411883
负责人：
JASON K KIM
金额：
$ 40.45万
依托单位：
UNIV OF MASSACHUSETTS MED SCH WORCESTER
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-09-16 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10411883
关键词：
American Animals Award Beta Cell Biometry Blinded Blood Pressure Body Composition Body measure procedure Cardiac Cardiovascular Diseases Cardiovascular Physiology Cardiovascular system Cell Transplantation Cell physiology Chemistry Closure by clamp Communities Complement Complications of Diabetes Mellitus Conscious Databases Diabetes Mellitus Eating Echocardiography Energy Metabolism Enzyme-Linked Immunosorbent Assay Equipment Exercise Fee-for-Service Plans Glucose Glucose Clamp Goals Grant Heart Rate Histologic Histology Hormones Housing Human Hyperglycemia Image Immunodeficient Mouse In Vitro Individual Insulin Lipids M-Mode Echocardiography Measurement Measures Metabolic Metabolism Mission Modeling Molecular Molecular Analysis Monitor Morphology Mus Obesity Operations Research Operative Surgical Procedures Organ Pharmaceutical Preparations Phenotype Physiological Prevalence Procedures Process Program Development Quarantine Research Research Personnel Resolution Resources Serum Services Signal Pathway Signal Transduction Standardization Structure Structure of beta Cell of islet Surgical Models Techniques Telemetry Tissue Sample Tissues Training Transgenic Mice Transplantation United States National Institutes of Health Vascular Endothelium chemokine community center cytokine diabetic experimental study glucose metabolism heart imaging humanized mouse in vivo in vivo imaging system instrument islet islet stem cells metabolic phenotype multidisciplinary novel operation programs protein metabolism research and development tool treadmill web site

项目摘要

Project Summary - Abstract The UMass Mouse Metabolic Phenotyping Center (MMPC) involves a multidisciplinary group of investigators at UMass who perform an array of novel and sophisticated metabolic, analytical and functional experiments using state-of-the-art equipment for the purpose of investigating transgenic mice useful for understanding obesity, diabetes and its complications. The goal of the UMass MMPC is to provide comprehensive metabolic and functional characterization of transgenic mice using unique and standardized techniques mostly involving in vivo and physiological setting that are complemented by analytical experiments using serum/tissue samples and measure of islet and cardiovascular functions in mice. The UMass MMPC consists of 2 Phenotyping Cores. The Metabolism Core applies hyperinsulinemic-euglycemic clamp to assess insulin action and glucose metabolism in individual organs, hyperglycemic clamp to assess pancreatic β-cell functions in conscious mice, metabolic cages to assess energy expenditure, activity and food intake, 1H-MRS to measure body composition, exercise studies using treadmill, and blinded drug trial studies. The Analytical and Functional Core consists of the complementary 3 sub-Cores. Analytical sub-Core uses Luminex and Cobas Chemistry Analyzer to provide a high-throughput and multiplexed measurement of serum/tissue levels of hormones, cytokines and metabolites, and performs molecular experiments to examine insulin/metabolic signaling. The Islet sub-Core performs elegant islet isolation and ex vivo and in vitro analysis of islet function and histology, and molecular analysis. The Cardiovascular sub-Core uses state-of-the-art Vevo2100 In Vivo Imaging System to conduct echocardiography and tissue Doppler imaging for cardiac structure and function analyses, telemetry to examine blood pressure and heart rates, standardized techniques to assess vascular and endothelial functions, and various elegant surgical models of cardiovascular diseases. Mice provided by the users are overseen by the Animal Core that processes the receiving, quarantine, and housing of mice. The Humanized Mouse Cell Transplantation and Assessment sub-Core will service the MMPC community by offering humanized mice and elegant technique to assess in vivo function of transplanted human islets and stem cell-derived β-cells in immunodeficient mice. Lastly, administrative, financial and scientific operation, research & development program, and website & database are managed by the Administrative Core. Overall, the overarching goal of the UMass MMPC is to provide comprehensive characterization of mice using state-of-the-art equipment and elegant and standardized techniques as an important scientific resource to support the mission of the National MMPC consortium program.

项目摘要 - 摘要 UMass小鼠代谢表型中心（MMPC）涉及一组多学科研究者 UMass，他们使用一系列新颖而复杂的代谢，分析和功能实验最先进的设备是为了研究转基因小鼠的目的，可用于了解肥胖症，糖尿病及其并发症。 UMass MMPC的目标是提供全面的代谢和使用独特和标准化技术对转基因小鼠的功能表征主要涉及体内通过使用血清/组织样品和小鼠胰岛和心血管功能的度量。 UMass MMPC由2个表型核心组成。这代谢核心采用高胰岛素 - 糖血糖夹来评估胰岛素作用和葡萄糖代谢在单个器官中，高血糖夹评估有意识小鼠的胰腺β细胞功能，代谢评估能量消耗，活动和食物摄入量的笼子，1H-MRS来测量身体成分，运动使用跑步机和盲毒试验研究的研究。分析和功能核心由完全3个子核。分析子核使用Luminex和Cobas化学分析仪提供高通量和多路复用测量的血清/组织水平的尸体，细胞因子和代谢产物的水平，并进行分子实验以检查胰岛素/代谢信号传导。胰岛亚核表现优雅胰岛分离和离体以及胰岛功能和组织学的体外分析以及分子分析。心血管次核使用最先进的VEVO2100体内成像系统进行超声心动图以及用于心脏结构和功能分析的组织多普勒成像，检查血压和心率，评估血管和内皮功能的标准化技术以及各种优雅心血管疾病的手术模型。用户提供的小鼠由动物核心监督处理小鼠的接收，隔离和住房。人源化的小鼠细胞移植和评估子核将通过提供人性化的老鼠和优雅的技术来为MMPC社区提供服务评估免疫缺陷小鼠中移植的人类胰岛和干细胞衍生的β细胞的体内功能。最后，行政，财务和科学运营，研发计划以及网站＆数据库由管理核心管理。总体而言，UMass MMPC的总体目标是使用最先进的设备以及优雅而标准化的小鼠进行全面的表征技术是支持国家MMPC联盟计划任务的重要科学资源。