GENETIC LINKAGE MAPPING IN RHESUS MACAQUES

恒河猴的遗传连锁图谱

• 批准号: 8172644
• 负责人: JEFFREY A. ROGERS
• 金额: $ 14.5万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172644
• 关键词: Animal Model; Animals; Biomedical Research; Chromosome Mapping; Chromosomes; Computer Retrieval of Information on Scientific Projects Database; DNA Sequence; Family; Funding; Generations; Genetic Polymorphism; Genome; Genome Mappings; Genomics; Grant; Human; Human Genome; Institution; Macaca mulatta; Maps; Microsatellite Repeats; Oregon; Primates; Research; Research Personnel; Research Project Grants; Resources; Source; United States National Institutes of Health; base; density; genetic analysis; genetic linkage; genetic linkage analysis; human disease; nonhuman primate; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Rhesus macaques are the most commonly used nonhuman primate in biomedical research. This species is used as a model organism for a wide range of human diseases, and it is widely acknowledged that genomic information, including genetic linkage maps, for rhesus monkeys would constitute an important resource for a large number of biomedical studies. This project is developing a resource (a genetic linkage map) that will make rhesus monkeys more valuable for a wide variety of research projects, especially the analysis of the genetic basis of common human diseases. The objective is to produce a linkage map of the rhesus genome with average spacing among markers of less than 5 centiMorgans. The markers (polymorphisms) to be used to map the rhesus chromosomes will consist of two types: a) human microsatellite loci already mapped in the humane genome, and b) novel rhesus-derived microsatellites identified and characterized using the rhesus whole genome DNA sequence. Using a significant number of human loci to map rhesus chromosomes will produce a rhesus genetic map that can be readily compared with the human genome map, while adding rhesus-derived loci will increase marker density and thus the information content. We have identified several multi-generation families of rhesus monkeys that are suitable for linkage analysis. More than 1000 rhesus monkeys are used for this project, including animals from the Oregon National Primate Research Center colony and the SNPRC colony.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 猕猴是生物医学研究中最常用的非人类灵长类动物。 该物种被用作多种人类疾病的模型生物，并且广泛认为，恒河猴的基因组信息（包括遗传链接图）将构成大量生物医学研究的重要资源。 该项目正在开发一个资源（遗传链接图），该资源将使恒河猴在各种研究项目中更有价值，尤其是对常见人类疾病的遗传基础的分析。 目的是生成恒河基因组的连锁图，在少于5厘米的标记中平均间距。 用于绘制恒河体染色体的标记（多态性）将由两种类型组成：a）已经映射在人类基因组中的人类微卫星基因座，b）新型恒河猴衍生的微片粒，使用恒河猴全基因组DNA序列鉴定并表征了恒河猴。 使用大量的人基因座来映射恒河体染色体将产生恒河类遗传图，与人类基因组图相比，可以很容易地与人类基因组图相比，同时添加恒河猴衍生的基因座将增加标记密度，从而增加信息含量。 我们已经确定了几个适合连锁分析的恒河猴的多代家族。 该项目使用了1000多只恒河猴，包括来自俄勒冈州国家灵长类动物研究中心殖民地和SNPRC殖民地的动物。