IMMUNOHISTOCHEMICAL IDENTIFICATION OF NONHUMAN PRIMATE ANTIGENS

非人灵长类抗原的免疫组织化学鉴定

• 批准号: 8172800
• 负责人: Susan V. Westmoreland
• 金额: $ 6.32万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172800
• 关键词: Acquired Immunodeficiency Syndrome; Antibodies; Antigens; Cebidae; Cells; Comparative Pathology; Computer Retrieval of Information on Scientific Projects Database; Development; Diagnostic; Experimental Pathology; Formalin; Freezing; Funding; Grant; Housing; Human; Immunohistochemistry; Infectious Agent; Institution; Macaca; Methods; Monkeys; Paraffin Embedding; Pathology; Postdoctoral Fellow; Protocols documentation; Research; Research Personnel; Resources; Source; Testing; Tissues; United States National Institutes of Health; Work; graduate student; improved; investigator training; molecular pathology; nonhuman primate; research and development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In order to improve our diagnostic pathology capabilities, we systematically evaluate commercially available antibodies directed against human cells/tissues and infectious agents for application in the nonhuman primate species housed at the NEPRC. Antibodies are selected for potential applications in diagnostic and/or experimental pathology, and for reactivity in either snap-frozen or formalin-fixed, paraffin-embedded tissues. In addition, the Division of Comparative Pathology receives multiple requests each year to test antibodies and develop IHC protocols for investigators in our Division, other NEPRC Divisions, and collaborating investigators. Thus, research and development to identify antibodies for immunohistochemistry in nonhuman primates is an important activity of the Molecular Pathology Core, and has contributed to the development of a diverse repertoire of IHC antibodies that work consistently in macaque tissue. In one study we were able to identify 21 of 21 antigens in 4 macaque species and 17 of 21 in New World monkeys, and during 2005, we developed immunohistochemistry protocols for 21 new antibodies that were cross-reactive in monkey tissues. The Division of Comparative Pathology also trains investigators, technicians, postdoctoral fellows and graduate students in immunohistochemistry methods. AIDS related.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 为了提高我们的诊断病理能力，我们系统地评估针对人类细胞/组织的商业可用抗体，并具有传染性剂，以应用于NEPRC的非人类灵长类动物。 选择用于诊断和/或实验病理学的潜在应用，以及用于快速冻结或福尔马林固定的石蜡包含的组织中的反应性。 此外，比较病理学的划分每年收到多个请求，以测试抗体并为我们部门的研究人员，其他NEPRC部门和合作调查人员开发IHC方案。 因此，鉴定非人类灵长类动物免疫组织化学抗体的研究和开发是分子病理核心的重要活性，并有助于开发在猕猴组织中始终如一地工作的IHC抗体曲目。 在一项研究中，我们能够在4种猕猴中鉴定21种抗原中的21种，而在新世界猴子中有21种中的17种，在2005年期间，我们开发了21种新抗体的免疫组织化学方案，这些抗体在猴子组织中具有交叉反应。 比较病理学的部门还培训了免疫组织化学方法的研究人员，技术人员，博士后研究员和研究生。 与艾滋病有关。