Studies of the Cochlear Nucleus Granule Cell Domain

耳蜗核颗粒细胞域的研究

基本信息

批准号：
7426775
负责人：
DAVID K. RYUGO
金额：
$ 33.4万
依托单位：
JOHNS HOPKINS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-09-01 至 2011-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7426775
关键词：
Acoustics Affective Air Animals Arousal Auditory Auditory area Auditory system Behavioral Binding Brain Cell Nucleus Cervical spinal cord structure Characteristics Chemistry Clinical Cochlea Cochlear nucleus Code Collaborations Cues Data Detection Dyes Ear Electron Microscopy Electrons Emotions External Ear Family Goals Grant Head Hearing Inferior Colliculus Injection of therapeutic agent Investigation Knowledge Learning Ligands Light Localized Mediating Memory Methods Microscopic Modality Motion Nature Neurons Neurotransmitter Receptor Neurotransmitters Numbers Operative Surgical Procedures Organism Pattern Personal Satisfaction Polymers Pontine structure Positioning Attribute Procedures Process Property Proprioception Rattus Recording of previous events Relative (related person)Research Sensorimotor functions Series Shapes Site Skeletal muscle structure of neck Sound Localization Source Spinal Staining method Stains Structure Structure of nucleus cuneatus Surface Synapses System Techniques Technology Testing Time Tinnitus Tracer Trigeminal Nuclei Vestibular Nerve Work biodegradable polymer cell type dorsal cochlear nucleus external ear auricle granule cell insight interest nanoparticle particle receptor relating to nervous system response somatosensory sound therapy design uptake vibration visual information

项目摘要

DESCRIPTION (provided by applicant): Perception of sound is attributed to the pattern of neural activity within the central auditory system. The auditory system is typically defined as a series of structures that are connected directly or indirectly to the cochlea. The significance of a sound, however, depends not only on spectral and temporal characteristics as mediated through the auditory system, but also on the past history and behavioral state of the animal. In other words, sound processing should also include non-auditory systems that would convey sensorimotor functions, emotion, learning, and memory. In this application, we plan to study the nature of non-auditory projections to the cochlear nucleus in order to gain insight into how different systems influence our perception of sound. We propose that the granule cell domain of the cochlear nucleus represents a key site for the integration of mutimodal influences. Preliminary data reveals that somatosensory, vestibular, pontine, reticular, and descending auditory projections access the granule cell domain. We will use anterograde and retrograde tract tracing methods in conjunction with light and electron microscopy to identify projecting neurons and their circuits, immunocytochemical staining procedures to reveal the chemistry of the different terminals, and electrophysiological recording techniques to characterize the response properties of neurons projecting to this region. We will apply nanoparticle technology to explore the use of biodegradable polymers with the goal of creating a new line of neuronal tracers for this study. The possibility of attaching ligands to the surface of the particles could enhance tracer uptake for the selective targeting of specific families of neurons. The data from this research will provide new knowledge on the synaptic organization of a highly integrative structure in the auditory system, and should yield insights into how diverse neural systems shape the coding process for hearing. As we learn about how the somatosensory system modulates hearing, we will gain insight into somatic tinnitus that could contribute to the design of treatment strategies and a better understanding of tinnitus in general.

描述（由申请人提供）：声音的感知归因于中枢听觉系统内的神经活动模式。听觉系统通常被定义为一系列直接或间接连接到耳蜗的结构。然而，声音的重要性不仅取决于通过听觉系统介导的频谱和时间特征，还取决于动物的过去历史和行为状态。换句话说，声音处理还应该包括传达感觉运动功能、情感、学习和记忆的非听觉系统。在此应用中，我们计划研究耳蜗核的非听觉投射的性质，以便深入了解不同的系统如何影响我们对声音的感知。我们认为耳蜗核的颗粒细胞域代表了多模式影响整合的关键位点。初步数据显示，体感、前庭、脑桥、网状和下降的听觉投射进入颗粒细胞域。我们将使用顺行和逆行束追踪方法，结合光学和电子显微镜来识别投射神经元及其回路，使用免疫细胞化学染色程序来揭示不同末端的化学性质，并使用电生理记录技术来表征投射到该神经元的反应特性。地区。我们将应用纳米颗粒技术来探索可生物降解聚合物的用途，目标是为本研究创建新的神经元示踪剂系列。将配体附着到颗粒表面的可能性可以增强示踪剂的吸收，从而选择性地靶向特定的神经元家族。这项研究的数据将提供有关听觉系统中高度整合结构的突触组织的新知识，并应深入了解不同的神经系统如何塑造听力的编码过程。当我们了解体感系统如何调节听力时，我们将深入了解体性耳鸣，这可能有助于设计治疗策略并更好地理解耳鸣。