IDENTIFICATION OF PROTEINS THAT REGULATE THE SIN3A HISTONE DEACETYLASE COMPLEX

调节 SIN3A 组蛋白脱乙酰酶复合物的蛋白质的鉴定

• 批准号: 8171342
• 负责人: PAUL D. KAUFMAN
• 金额: $ 0.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171342
• 关键词: Breast; Cell Proliferation; Chromatin; Complex; Computer Retrieval of Information on Scientific Projects Database; Data; Funding; Gene Expression; Gene Expression Regulation; Genome Stability; Grant; Growth; Histone Deacetylase; Histones; Institution; Mass Spectrum Analysis; Neoplasm Metastasis; Play; Post-Translational Protein Processing; Proteins; Research; Research Personnel; Resources; Role; Source; Tumor Suppressor Proteins; United States National Institutes of Health; cell type; gene repression; human BRMS1 protein

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Histone post-translational modifications play a pivotal role in gene regulation, chromatin packaging and cellular differentiation. The histone deacetylase (HDACs) mSin3A complex is involved in transcriptional repression and contains multiple subunits that have been implicated in growth control, including HDAC 1 and 2, the Breast Suppressor of Metastasis 1 proteins (BRMS1 and BRMS1L1), and the p33/ING1 tumor suppressor proteins. However, our preliminary data suggest that mSin3A complex may be compositionally diverse in different cell types and under different growth conditions. Isolation and characterization of different Sin3A subunits is essential for elucidating the role of the various mSin3A complexes. Using mass spectroscopy, we aim to identify associated proteins, and determine how they interact with other subunits. These data will guide functional studies to determine effects on cell proliferation, gene expression and genomic stability.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 组蛋白翻译后修饰在基因调控、染色质包装和细胞分化中发挥着关键作用。 组蛋白脱乙酰酶 (HDAC) mSin3A 复合体参与转录抑制，并包含多个与生长控制有关的亚基，包括 HDAC 1 和 2、乳腺癌转移抑制蛋白 1（BRMS1 和 BRMS1L1）以及 p33/ING1 肿瘤抑制蛋白。 然而，我们的初步数据表明，mSin3A 复合物在不同的细胞类型和不同的生长条件下可能具有不同的组成。 不同 Sin3A 亚基的分离和表征对于阐明各种 mSin3A 复合物的作用至关重要。 使用质谱法，我们的目标是识别相关蛋白质，并确定它们如何与其他亚基相互作用。 这些数据将指导功能研究，以确定对细胞增殖、基因表达和基因组稳定性的影响。