ENGINEERING OF BICELLES FOR PULSED DIPOLAR ESR STUDY ON MEMBRANE PROTEINS

用于膜蛋白脉冲偶极 ESR 研究的 Bicelle 工程

• 批准号: 8172178
• 负责人: ELKA R GEORGIEVA
• 金额: $ 0.36万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172178
• 关键词: Biological; Computer Retrieval of Information on Scientific Projects Database; Electron Spin Resonance Spectroscopy; Engineering; Environment; Freezing; Funding; Goals; Grant; Institution; Lipids; Membrane; Membrane Proteins; Particle Size; Physiologic pulse; Proteins; Research; Research Personnel; Resources; Sampling; Solutions; Source; Structure; Time; United States National Institutes of Health; base; mimetics

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bicelles provide an environment wherein proteins can interact with a bilayer structure composed of biologically competent lipids, packaged into a particle size that is sufficiently small to allow for rapid tumbling times in solution for NMR and for high protein concentrations and uniform distributions in the frozen samples used in pulsed dipolar ESR spectroscopy. According to this, it was necessary to engineer suitable bicelles and explore their utility for studies of protein-membrane interactions. We pursued the following specific goal: (1) to prepare bicelles as biological membrane mimetics, based on different compositions and ratios of long chain to short chain lipids; (2) to characterize their size and stability with respect to their loading with protein.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 双层提供了一个环境，其中蛋白可以与由具有生物学胜任的脂质组成的双层结构相互作用，该结构包装成粒径，该蛋白质足够小，可以在NMR的溶液中快速滚动的时间，以及在脉冲二极管ESR ESR ESR频谱中使用的高蛋白质浓度和均匀分布的溶液。据此，有必要设计合适的双丝，并探索其用于研究蛋白质膜相互作用的实用性。我们追求以下具体目标：（1）基于长链与短链脂质的不同组成和比率，将双丝材料作为生物膜模拟物进行准备； （2）表征其与蛋白质负荷相对于蛋白质的稳定性。