MECHANISM OF THE ENERGY-STORING REACTIONS IN PHOTOSYNTHETIC ORGANISMS

光合生物的储能反应机制

• 批准号: 8168795
• 负责人: Robert Eugene Blankenship
• 金额: $ 0.17万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-10 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168795
• 关键词: Appearance; Bacterial Genome; Binding; Biochemical; Biological; Carrier Proteins; Complex; Computer Retrieval of Information on Scientific Projects Database; Electron Spin Resonance Spectroscopy; Electron Transport; Evolution; Fluorescence; Funding; Genomics; Goals; Grant; Horizontal Gene Transfer; Institution; Kinetics; Lasers; Life; Measurement; Membrane; Metabolism; Molecular; Molecular Evolution; Multienzyme Complexes; Nitrogen Fixation; Organism; Photons; Photosynthesis; Pigments; Proteins; Reaction; Recording of previous events; Research; Research Personnel; Resources; Source; System; Techniques; Thermodynamics; United States National Institutes of Health; chemical reaction; flash photolysis; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The chemical reactions leading to long-term energy storage in photosynthetic systems take place within the membrane-bound reaction center complex and an associated group of proteins that make up an electron transport chain. One of the central goals of our research is to identify the molecular parameters responsible for the fact that essentially every photon absorbed by the system leads to stable products. To this end, we do a variety of kinetic, thermodynamic and structural measurements on antenna complexes, reaction centers, electron transport proteins and isolated pigments, using a number of techniques, including ultrafast laser flash photolysis and UV-VIS, fluorescence and electron spin resonance spectroscopies, as well as biochemical and molecular biological analysis. The appearance of photosynthesis and other metabolic processes such as nitrogen fixation had profound effects on the evolution of advanced life on Earth. Our analysis of whole bacterial genomes has revealed that these metabolic processes have complex evolutionary histories, including substantial horizontal gene transfer. We have also used a combination of genomic, molecular evolution techniques and biochemical analysis to identify and characterize previously unknown enzyme complexes with novel activities.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 导致光合系统长期储能的化学反应发生在膜结合的反应中心络合物中，以及组成电子传输链的相关蛋白质组。我们研究的核心目标之一是确定负责以下事实的分子参数，即系统吸收的每个光子都会导致稳定的产品。为此，我们使用多种技术（包括超快激光闪光光解和UV-VIS，荧光和电子自旋共循环光谱和生物学分析，以及生物学分析，以及生物学分析。 光合作用和其他代谢过程（例如氮固定）的出现对地球上高级生命的演变产生了深远的影响。我们对整个细菌基因组的分析表明，这些代谢过程具有复杂的进化史，包括大量水平基因转移。我们还使用了基因组，分子进化技术和生化分析的组合来识别和表征与新活性的先前未知酶复合物。