COBRE: OK MED RES FOUND: CORE I: IN VITRO MICROSCOPY CORE

COBRE：确定医学研究成果：核心 I：体外显微镜核心

• 批准号: 8168454
• 负责人: FLOREA LUPU
• 金额: $ 13万
• 依托单位: OKLAHOMA MEDICAL RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-08 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168454
• 关键词: Arts; Biology; Cardiovascular system; Complex; Computer Retrieval of Information on Scientific Projects Database; Confocal Microscopy; Electrons; Equipment; Experimental Designs; Freeze Fracturing; Freeze Substitution; Freezing; Funding; Grant; Image; In Vitro; Institution; Label; Light; Methods; Microscopy; Play; Preparation; Research; Research Personnel; Resolution; Resources; Role; Sampling; Source; Specialist; Specimen; Techniques; Technology; Three-Dimensional Imaging; Transmission Electron Microscopy; United States National Institutes of Health; base; experience; pressure; programs; skills

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Advanced microscopy techniques represent major investigational approaches in each of the proposed projects of our multi-disciplinary program. The choice of the most appropriate method can be very complex, and the techniques involved in sample preparation require considerable skill. Therefore the In Vitro Microscopy Core will play a particularly important role in this collaborative program. The Core will provide state-of-the-art expertise in various steps of the projects, from experimental design and specimen preparation, to image capture and analysis of high-resolution light and electron micrographs. The In Vitro Microscopy Core will be based in the recently established Imaging Facility of the Cardiovascular Biology Research Program, under the direction of Dr. Florea Lupu. An experienced imaging specialist, Dr. Andrew Westmuckett will devote 50% of his effort towards the activities described in this proposal. The core has the expertise and the equipment to perform a diversity of techniques, ranging from brightfield histological analysis to confocal microscopy and 3D imaging, from standard transmission electron microscopy to immunogold labeling and advanced cryo-technologies (cryoimmunogold labeling, freeze substitution, high pressure freezing, and freeze fracture and deep-etching).

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 先进的显微镜技术代表了我们多学科计划的每个拟议项目的主要研究方法。选择最合适的方法可能非常复杂，并且样品制备涉及的技术需要相当高的技能。因此，体外显微镜 Core 将在这个合作项目中发挥特别重要的作用。 该核心将在项目的各个步骤中提供最先进的专业知识，从实验设计和样本制备，到高分辨率光学和电子显微照片的图像捕获和分析。 体外显微镜核心将设在最近建立的心血管生物学研究计划成像设施中，由 Florea Lupu 博士指导。 Andrew Westmuckett 博士是一位经验丰富的影像专家，他将投入 50% 的精力用于本提案中描述的活动。 该核心拥有执行各种技术的专业知识和设备，从明场组织学分析到共焦显微镜和 3D 成像，从标准透射电子显微镜到免疫金标记和先进的冷冻技术（冷冻免疫金标记、冷冻替代、高压冷冻） ，以及冷冻断裂和深蚀刻）。