Intravital Multiphoton Microscope

活体多光子显微镜

• 批准号: 7794743
• 负责人: FLOREA LUPU
• 金额: $ 50万
• 依托单位: OKLAHOMA MEDICAL RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-22 至 2011-04-21
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794743
• 关键词: Animal Organ; Arthritis; Autoimmunity; Bacterial Infections; Biology; Cardiovascular system; Cell Aging; Cells; Collection; Complement; Detection; Developmental Biology; Dimensions; Disease; Fluorescent Probes; Foundations; Free Radicals; Funding; Image; Immersion Investigative Technique; Immunobiology; Immunology; Inflammation; Label; Lasers; Life; Light; Magnetic Resonance; Malignant Neoplasms; Medical Research; Microscope; Minor; Molecular; Neurodegenerative Disorders; Oklahoma; Optics; Organ; Organ Culture Techniques; Penetration; Photobleaching; Photons; Physiologic pulse; Resolution; Resources; Sapphire; Scanning; Slice; Specimen; System; Technology; Thick; Tissues; Transgenic Mice; Visible Radiation; Water; X-Ray Computed Tomography; cell age; fluorescence microscope; fluorophore; human disease; insight; instrument; mouse model; multi-photon; novel; programs

DESCRIPTION (provided by applicant): We are requesting funds for a multiphoton laser scanning microscope system. The microscope system consists of: 1) a Zeiss 710 NLO multi-photon imaging system with 3 spectral detection channels, five laser lines for visible light excitation confocal imaging and prepared for femtosecond pulsed NIR beam; 2) a Zeiss Axio Imager Z1 motorized fluorescence microscope with DIC and high resolution water immersion optics; and 3) a Coherent Chameleon free Ultrafast II Ti:Sapphire laser system. The microscope will be used to examine live small animals, organ cultures, tissue slices, whole-mount organs and isolated tissues that have been labeled with multiple parameter-indicating fluorescent probes. Two- and 3-photon imaging will minimize photo-damage and photobleaching, maximize penetration into thick specimens, permit excitation of UV-absorbing fluorophores with red and near infrared light, and optimize photon collection efficiency. The new system is sought to relieve the critically high demand for nondestructive imaging of living cells and tissues with high resolution in 3-dimensions. The multiphoton excitation microscope will provide a strong complement to our current imaging resources, which include high-resolution magnetic resonance and computerized tomography imaging. The microscope will also extend the value of a number of transgenic mouse models being studied at the Oklahoma Medical Research Foundation (OMRF). Ten major users and seven minor users will immediately employ the multiphoton microscope in fifteen independent or collaborative projects in the Programs of Arthritis and Immunology, Cardiovascular Biology, Immunobiology and Cancer, Free Radicals and Aging, and Cell, Molecular & Developmental Biology at OMRF. It is anticipated that the instrument will allow us to access novel insights in the pathological mechanisms of several diseases. A partial list of human disease studies at the OMRF that will benefit from this technology include cardiovascular and thrombotic disorders, inflammation and autoimmunity, cancer, neurodegenerative diseases and bacterial infections.

描述（由申请人提供）：我们正在为多光子激光扫描显微镜系统申请资金。显微镜系统包括： 1）蔡司 710 NLO 多光子成像系统，具有 3 个光谱检测通道、5 条激光线，用于可见光激发共焦成像，并为飞秒脉冲近红外光束做好准备； 2) 配备 DIC 和高分辨率水浸光学器件的 Zeiss Axio Imager Z1 电动荧光显微镜； 3) 一个 Coherent Chameleon free Ultrafast II Ti:Sapphire 激光系统。该显微镜将用于检查活体小动物、器官培养物、组织切片、整体器官和已用多个参数指示荧光探针标记的分离组织。两光子和三光子成像将最大限度地减少光损伤和光漂白，最大限度地渗透到厚样本中，允许用红光和近红外光激发紫外线吸收荧光团，并优化光子收集效率。新系统旨在满足对活细胞和组织的三维高分辨率无损成像的极高需求。多光子激发显微镜将为我们当前的成像资源提供强有力的补充，其中包括高分辨率磁共振和计算机断层扫描成像。该显微镜还将扩展俄克拉荷马州医学研究基金会（OMRF）正在研究的许多转基因小鼠模型的价值。十个主要用户和七个次要用户将立即在 OMRF 的关节炎和免疫学、心血管生物学、免疫生物学和癌症、自由基和衰老以及细胞、分子和发育生物学项目的 15 个独立或合作项目中使用多光子显微镜。预计该仪器将使我们能够对几种疾病的病理机制获得新的见解。 OMRF 的部分人类疾病研究将受益于这项技术，包括心血管和血栓性疾病、炎症和自身免疫、癌症、神经退行性疾病和细菌感染。