Mouse Genetics

小鼠遗传学

• 批准号: 7985702
• 负责人: JAMES O PHILLIPS
• 金额: $ 15.49万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7985702
• 关键词: Affect; Auditory; Auditory Brainstem Responses; Behavior; Behavioral; Biological Models; Breeding; Caring; Child; Collaborations; Communication; Data Analyses; Defect; Development; Disease; Drosophila genus; Embryonic Development; Equilibrium; Fostering; Genes; Genetic; Genetic Models; Genetic Techniques; Genomics; Genotype; Goals; Health; Hearing; Human; Human Resources; Induced Mutation; Injection of therapeutic agent; Instruction; Knowledge; Lead; Lentivirus Vector; Modification; Molecular Genetics; Motor; Mouse Strains; Mus; Notification; Organism; Perinatal; Physiological; Prevention; Process; Production; Proteomics; Protocols documentation; Research; Research Personnel; Research Project Grants; Resources; Role; Rotarod Performance Test; Scientist; Sensory; Services; System; Techniques; Technology; Testing; Training; Transfection; Transgenic Organisms; United States; Weaning; Zebrafish; behavior test; body system; computerized tools; deafness; efficacy research; experience; hearing impairment; improved; meetings; mouse model; mutant; neonate; operation; vestibulo-ocular reflex

PROJECT SUMMARY (See instructions): Hearing loss is the most frequent sensory defect in humans. Congenital, perinatal or eariy onset hearing loss occurs in approximately 7 out of 1000 neonates in the United States. In approximately half of the children born with severe hearing impairment, a genetic contribution is suspected. The powerful molecular and genetic techniques available in zebrafish and mouse combined with the functional similarities between mouse and human audition make these organism useful model systems for studying deafness. Core D supports users of genetic models to study genes affecting auditory, vestibular and olfactory system function. We provide technical support in the form of expert mouse husbandry (receiving, setting up crosses, weaning, fostering, background transfers, etc), genotyping, and notification/delivery of requested mice to the users. Core D also supports several facilities for behavioral and physiological analyses of mutants including auditory brainstem responses (ABR), simple motor tests, rotarod testing, and vestibulo-ocular reflex (VOR) analysis. Other services include access to lentiviral transfection technologies and resources for genomic and proteomic data analysis. Advice and training is available to all users from a knowledgeable and helpful Core staff. The Genetics Core will provide greater efficiency for researchers and promote new collaborations between investigators. Quarteriy user meetings provide information on Core resources, discussion of protocols, and talks by Core users on their own research projects currently being pursued. Our goal is to facilitate the use of genetic models to broaden our knowledge of communicafive disorders toward improving human health.

项目摘要（请参阅说明）： 听力损失是人类最常见的感觉缺陷。在美国，在1000名新生儿中，大约7个发生了先天，围产期或耳朵发作的听力损失。在大约一半的患有严重听力障碍的孩子中，怀疑遗传贡献。斑马鱼和小鼠中可用的强大分子和遗传技术与小鼠和人类试听之间的功能相似性相结合，使这些生物体有用的模型系统用于研究耳聋。 核心D支持遗传模型的用户研究影响听觉，前庭和嗅觉系统功能的基因。我们以专家饲养的形式提供技术支持（接收，设置十字架，断奶，寄养，背景转移等），基因分型以及向用户提供所请求的小鼠的通知/传递。核心D还支持多种对突变体的行为和生理分析的设施，包括听觉脑干反应（ABR），简单的运动测试，Rotarod测试和前庭 - 眼反射（VOR）分析。其他服务包括访问慢病毒转染技术以及用于基因组和蛋白质组学分析的资源。来自知识渊博且有用的核心人员的所有用户都可以使用建议和培训。 遗传学核心将为研究人员提供更大的效率，并促进研究人员之间的新合作。季度用户会议提供了有关核心资源，协议讨论以及核心用户对目前正在追求的研究项目的讨论的信息。我们的目标是促进使用遗传模型来扩大我们对沟通障碍的了解，以改善人类健康。