THE EFFECTS OF AGES ON CELL BEHAVIOR WITHIN A 3D VASCULAR TISSUE CONSTRUCT

年龄对 3D 血管组织结构内细胞行为的影响

• 批准号: 8167974
• 负责人: Heather Fahlenkamp
• 金额: $ 7.31万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167974
• 关键词: Advanced Glycosylation End Products; Area; Arterial Fatty Streak; Atherosclerosis; Award; Basement membrane; Blood Vessels; Bovine Serum Albumin; Cell physiology; Collaborations; Collagen Type IV; Complications of Diabetes Mellitus; Computer Retrieval of Information on Scientific Projects Database; Development; Diabetes Mellitus; Endothelial Cells; Funding; Future; Genus Capra; Glucose; Goals; Grant; Human; Hyperglycemia; Immune; In Vitro; Individual; Inflammatory; Institution; Lead; Link; Mentors; Modeling; Modification; Notification; Physiological; Pilot Projects; Research; Research Personnel; Research Project Grants; Resources; Source; Supporting Cell; System; Techniques; Therapeutic; Tissue Model; Tissues; United States National Institutes of Health; Work; age effect; cell behavior; cell motility; meetings

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Effect of AGEs on Cell Behavior Within a 3D Vascular Tissue Construct This start of this project was delayed and the work has been limited due to the delay of formal funding from NIH for the Pilot Project. Since notification of the Pilot Project award in early October, I have attended an advisory board meeting, as well as monthly PJI meetings at OUHSC. For the EAC/IAC meeting in early November, I presented my research highlights for past work with the 3D vascular tissue model that led to the development of my pilot project. I received some useful information from the committee that has been used to further develop my current research project. For the monthly PJI meetings, each PJI is taking a turn to present current research findings to the group, which is followed by a group discussion. Dr. Don Capra has also been attending the monthly PJI meetings to serve as a research mentor. The individual presentations and the group discussion allows me to understand the research areas of other PJIs that leads to more interaction and possible future collaborations. Progress on the pilot project includes modifications to the 3D vascular tissue model so that it mimics physiological conditions associated with the vascular regions prone to atherosclerosis. This includes using human aortic endothelial cells and using type IV collagen to create a basement membrane to support the cells. Due to these changes, the system is being characterized and compared to the previous model. This includes functional analyses of the endothelial cells under both normal and inflammatory conditions associated with atherosclerosis. Additional work on the project includes the formation and characterization of advanced glycosylation end products (AGE). A proposed mechanism linking hyperglycemia to diabetic complications like atherosclerosis is the increase of advanced glycosylation end products formation. A standard technique using bovine serum albumin and glucose was used for in vitro AGE formation. The modified 3D vascular tissue model will be used to study the effect of AGE on endothelial cell function and immune cell migration and differentiation that would lead to atherosclerotic plaque formation. The goal of this project is to is to use the 3D vascular tissue construct to determine the underlying mechanisms associated with hyperglycemia and atherosclerosis in order to develop new preventative and/or therapeutic strategies.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 年龄对3D血管组织构建体内细胞行为的影响 该项目的开始延迟了，由于NIH为飞行员项目延迟了正式资金，因此工作受到限制。 自10月初通知飞行员项目奖以来，我参加了一次顾问委员会会议以及OUHSC的每月PJI会议。 在11月初的EAC/IAC会议上，我介绍了与3D血管组织模型过去合作的研究重点，该模型导致了我的飞行员项目的发展。 我收到了委员会的一些有用信息，这些信息已被用来进一步开发我当前的研究项目。 对于每月的PJI会议，每个PJI都会轮流向小组提供当前的研究结果，然后进行小组讨论。 唐·卡普拉（Don Capra）博士还一直在每月举行的PJI会议上担任研究导师。 各个演讲和小组讨论使我能够了解其他PJI的研究领域，这些研究领域会导致更多的互动和可能的未来合作。 试验项目的进展包括对3D血管组织模型的修改，以便模拟与容易发生动脉粥样硬化的血管区域相关的生理状况。 这包括使用人主动脉内皮细胞，并使用IV型胶原蛋白创建基底膜以支持细胞。 由于这些变化，系统正在表征并将其与先前的模型进行比较。 这包括在与动脉粥样硬化有关的正常和炎症条件下对内皮细胞的功能分析。 该项目的其他工作包括高级糖基化最终产品（年龄）的形成和表征。 提出的一种将高血糖与糖尿病并发症（如动脉粥样硬化）联系起来的机制是高级糖基化最终产物形成的增加。使用牛血清白蛋白和葡萄糖的标准技术用于体外年龄形成。 修饰的3D血管组织模型将用于研究年龄对内皮细胞功能和免疫细胞迁移和分化的影响，这将导致动脉粥样硬化斑块形成。 该项目的目的是使用3D血管组织构建体来确定与高血糖和动脉粥样硬化相关的潜在机制，以开发新的预防和/或治疗策略。