LOUISIANA COBRE: OBESITY & DIABETES RES: CELL BIOLOGY & BIOIMAGING CORE

路易斯安那州 COBRE：肥胖

基本信息

批准号：
8167950
负责人：
DAVID L BURK
金额：
$ 13.83万
依托单位：
LSU PENNINGTON BIOMEDICAL RESEARCH CTR
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-07-01 至 2011-06-30
项目状态：
已结题

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cell Biology and Bioimaging (CBB) Core (http://labs.pbrc.edu/cellbiology/index.htm) was organized in response to the specific needs of COBRE Project PIs and researchers at the Pennington Biomedical Research Center. Its purpose is to develop and provide state of the art bioimaging approaches to study the cell and molecular biology of biological processes in live and fixed cells and tissues, as well as physiological processes in vivo using micro CT. The CBB Core provides state of the art bioimaging and analytical instrumentation and support to facilitate data collection and analysis by COBRE Project PIs and PBRC faculty. Currently the CBBC is staffed by Dr. David H. Burk who serves as director, Dr. Barbara Kozak who serves as associate director and two research associates Ms. Courtney Cain and Mr. Drury Ingram. Funding of the Pennington COBRE in September 2006 brought the Cell Biology and Bioimaging Core into existence and it was organized around five major analytical platforms: a Zeiss LSM-510 meta multiphoton confocal, an upright epifluorescence microscope and an inverted epifluorescence microscope, a scanning fluorometer for live cell imaging, and a FACS Caliber analytical cell sorter. A primary focus in the first two years was to enhance our existing microscopy expertise and development new expertise in the areas of flow cytometry, immunohistochemistry, imaging and image analysis. Our overall goal is to enhance the competitiveness of our faculty by providing seamless access to these technologies. The CBBC is loosely divided into three sections imaging, histology & specimen preparation, and flow cytometry. Within the imaging section, the core has two multi-photon confocals, one fully motorized inverted system for high speed ratiometric imaging, an upright motorized system for fixed cell or tissue imaging, a multi-color laser TIRF rig, two manual scopes for routine examination of slides or multi-well plates (brightfield and fluorescence), and a digital pathology slide scanning system. The histology & specimen preparation section holds four cryostats, three rotary microtomes, an automated tissue processor, tissue embedding station, automated slide stainer/coverslipper, auto IHC stainer, and a laser microdissection system. The Core's flow cytometry section houses a BD FACSCalibur for 4 color analysis and an iCyt Reflection high speed cell sorter equipped within a BSL2 lamellar flow hood.

该子项目是利用该技术的众多研究子项目之一资源由 NIH/NCRR 资助的中心拨款提供。子项目和研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金，因此可以在其他 CRISP 条目中表示。列出的机构是对于中心来说，它不一定是研究者的机构。细胞生物学和生物成像 (CBB) 核心 (http://labs.pbrc.edu/cellbiology/index.htm) 的组织是为了响应 COBRE 项目 PI 和彭宁顿生物医学研究中心研究人员的具体需求。其目的是开发和提供最先进的生物成像方法，以研究活体和固定细胞和组织中生物过程的细胞和分子生物学，以及使用微型 CT 进行体内生理过程。 CBB 核心提供最先进的生物成像和分析仪器和支持，以促进 COBRE 项目 PI 和 PBRC 教员的数据收集和分析。目前，CBBC 的工作人员包括担任主任的 David H. Burk 博士、担任副主任的 Barbara Kozak 博士和两名助理研究员。考特尼·凯恩和德鲁里·英格拉姆先生。 2006 年 9 月，彭宁顿 COBRE 获得资助，形成了细胞生物学和生物成像核心，它围绕五个主要分析平台进行组织：蔡司 LSM-510 元多光子共焦、正置落射荧光显微镜和倒置落射荧光显微镜、扫描荧光计活细胞成像和 FACS Calibre 分析细胞分选仪。前两年的主要重点是增强我们现有的显微镜专业知识并开发流式细胞术、免疫组织化学、成像和图像分析领域的新专业知识。我们的总体目标是通过提供对这些技术的无缝访问来提高教师的竞争力。 CBBC 大致分为三个部分：成像、组织学和标本制备以及流式细胞术。在成像部分，核心有两个多光子共焦、一个用于高速比例成像的全电动倒置系统、一个用于固定细胞或组织成像的直立电动系统、一个多色激光 TIRF 装置、两个用于常规检查的手动示波器载玻片或多孔板（明场和荧光），以及数字病理载玻片扫描系统。组织学和标本制备部分拥有四个低温恒温器、三个旋转切片机、一个自动组织处理机、组织包埋站、自动载玻片染色机/盖玻片机、自动 IHC 染色机和激光显微切割系统。 Core 的流式细胞仪部分配有用于 4 色分析的 BD FACSCalibur 和配备在 BSL2 层状流罩内的 iCyt Reflection 高速细胞分选仪。