ONTOGENY OF INNATE IMMUNE RESPONSES AT MUCOSAL SURFACES

粘膜表面先天免疫反应的个体发生

• 批准号: 8168485
• 负责人: Octavio Alberto Gonzalez
• 金额: $ 30.75万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168485
• 关键词: Address; Age; Animals; Blood Circulation; Caribbean region; Characteristics; Computer Retrieval of Information on Scientific Projects Database; Controlled Study; Cross-Sectional Studies; Dental Schools; Development; Disease; Equilibrium; Event; Funding; Gene Expression; Gingiva; Grant; Growth; Homeostasis; Human; Immune; Immune response; Immune system; Individual; Infection; Inflammation; Institution; Kentucky; Macaca mulatta; Modeling; Molecular; Natural Immunity; Oral cavity; Periodontal Diseases; Periodontal Infection; Periodontitis; Play; Primates; Process; Puerto Rico; Reaction; Research; Research Personnel; Resolution; Resources; Role; Source; Surface; System; Technology; Tissues; United States National Institutes of Health; Universities; cohort; innovation; microbial; nonhuman primate; novel; oral infection; receptor; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The hallmark of the vertebrate immune system is its ability to maintain an equilibrium between reactivity and quiescence of responses, while geared to respond and adapt rapidly to noxious and potentially lethal challenge. The system must recognize the challenge, develop and express a response to control the challenge, and terminate the reaction in regulating the response. These reactions are the responsibility of components of innate immunity that include cellular receptors and biomolecules at mucosal surfaces and within the systemic circulation. Due to the complexity of the microbial challenge in the oral cavity, it is clear that the innate immune system must play a crucial role in regulating host responses to the commensal microbiota. This proposal engages a collaborative relationship to enable determination of these responses in a human-like model of oral infection and inflammation: (1) This proposal will focus on the use of a nonhuman primate (rhesus) model of periodontal infection and inflammation. It is built upon a unique resource, the Caribbean Primate Research Center (CPRC); (2) This proposal is a collaborative relationship between investigators at the University of Kentucky, the CPRC, and the University of Puerto Rico Dental School; (3) This proposal will provide novel information on innate immunity in cross-sectional studies related to age using various cohorts from young to aged animals, and related to the expression of periodontitis; and, (4) Using the nonhuman primate model of periodontal infection and disease, we will determine longitudinal changes in innate immune responses througout age of development of the individual, specifically related to the initiation, progression, and resolution of the disease process. This model provides for a controlled study of these processes that cannot be accomplished in humans, but provides a human-like model of infection, disease and host responses. The General Hypothesis for the project is that "Innate immune response components in gingival tissues vary with age and periodontal disease expression." This will enable the identification of crucial innate immune molecular events that contribute to gingival homeostasis. The studies proposed in this application will use an innovative strategy in 2 Specific Aims to interface a nonhuman primate model of periodontitis, with microarray gene expression technology to address these critical questions regarding the characteristics and ontogeny of innate responses in the oral cavity.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 脊椎动物免疫系统的标志在于其在反应性和反应静止之间保持平衡的能力，同时适应反应并迅速适应有害和潜在的致命挑战。 该系统必须认识到挑战，发展并表达以控制挑战的反应，并终止调节反应的反应。 这些反应是先天免疫成分的责任，包括粘膜表面和全身循环中的细胞受体和生物分子。 由于口腔中微生物挑战的复杂性，很明显，先天免疫系统必须在调节对共生微生物群的宿主反应中起着至关重要的作用。 该提案与协作关系具有协作关系，以在口腔感染和炎症模型中确定这些反应：（1）该提案将着重于使用牙周感染和炎症的非人类灵长类动物（Rhesus）模型。它建立在一个独特的资源基础上，即加勒比灵长类动物研究中心（CPRC）； （2）该建议是肯塔基大学，CPRC和波多黎各大学牙科学校的调查人员之间的合作关系； （3）该提案将提供有关使用各种同类群体到年龄动物的各种同龄人与年龄有关的横断面研究中先天免疫的新信息，并与牙周炎的表达有关； （4）使用牙周感染和疾病的非人类灵长类动物模型，我们将通过个人发展的年龄，与疾病过程的起始，进步和解决方案有关，先天免疫反应的纵向变化。 该模型提供了对人类无法完成的这些过程的对照研究，但提供了类似人类的感染，疾病和宿主反应的模型。该项目的一般假设是“牙龈组织中的先天免疫反应成分随年龄和牙周疾病表达而变化。” 这将使能够确定有助于牙龈稳态的关键先天免疫分子事件。本应用程序中提出的研究将在2个特定目的中使用创新策略，以将非人类灵长类动物的牙周炎模型与微阵列基因表达技术接触，以解决有关口腔中先天反应的特征和个体基础的这些关键问题。