DIETARY METHIONINE UPTAKE BY SPLANCHNIC REGION IN HEALTHY AND SICK CHILDREN

健康儿童和患病儿童内脏区域的膳食蛋氨酸摄取量

• 批准号: 8166704
• 负责人: Leticia Castillo
• 金额: $ 2.67万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-12-01 至 2010-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8166704
• 关键词: Adolescent; Affect; Amino Acids; Area; Arginine; Blood; Blood Circulation; Catabolism; Child; Childhood; Computer Retrieval of Information on Scientific Projects Database; Critical Illness; Cysteine; Data; Diet; Disease; Enteral; Essential Amino Acids; Funding; Glutamine; Grant; Homeostasis; Infant; Infusion procedures; Institution; Intestines; Intravenous; Leucine; Metabolism; Methionine; Newborn Infant; Nutritional; Organ; Pathologic; Patients; Pattern; Play; Population; Process; Protein/Amino Acids Nutrition; Protocols documentation; Research; Research Personnel; Resources; Role; Small Intestines; Source; Sulfur Amino Acids; Tissues; Tracer; United States National Institutes of Health; Venous; cystic fibrosis patients; uptake

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The splanchnic region plays a significant role in the homeostasis of whole body amino acid metabolism and selectively influences the pattern of absorbed amino acids that is subsequently released into the systemic circulation. During critical illness the splanchnic area is profoundly affected, and may influence amino acid metabolism. There are limited data on inter-organ amino acid exchange and the role of splanchnic uptake and catabolism of amino acids in critically ill patients. To determine parenteral and enteral amino acid requirements, it is necessary to determine the extent of the first pass disappearance of amino acids in the splanchnic region. We plan to study a group of healthy adolescents who will serve as controls for our critically ill adolescent group. Sulfur amino acid splanchnic uptake in healthy adolescents has not been determined and needs to be define. Hence enteral requirement of sulfur amino acids in sick children need to be defined. The first objective of this protocol is to conduct studies of methionine and cysteine splanchnic uptake in healthy children adolescents. The studies in healthy children will serve as controls for our critically ill population. We are requesting GCRC support to conduct our studies in the healthy children. We aim to conduct primed, constant simultaneous intravenous and enteral infusions of L-[1-13C] and L[2H2 ]methionine in a first set of studies and of L-[1-13C] and L[2H2 ] cysteine in a second set of studies in healthy children and to use these data to estimate the contribution of splanchnic sequestration to methionine and cysteine requirements. Primary Endpoint: The fraction of methionine or cysteine tracer that disappears in a first-pass through the splanchnic area This protocol is a continuation of our studies on sulfur amino acid metabolism in sick children. We have already conducted studies of methionine splanchnic uptake in critically ill infants, children and adolescents (Please see preliminary data). The splanchnic region plays a significant role in the homeostasis of whole body amino acid metabolism. The splanchnic area selectively influences the pattern of absorbed amino acids that is subsequently released into the systemic circulation. Intestinal amino acid metabolism plays an important role in modulating the entry of absorbed dietary amino acids into the portal circulation. Thus, the pattern of amino acids in the diet differs markedly from that in portal venous blood and does not reflect their availability to extraintestinal tissues. This concept has important implications for protein and amino acid nutrition. The extensive catabolism of dietary essential amino acids in the first pass by the small intestine, a large organ in the body, results in decreased nutritional efficiency. Thus, intestinal amino acid metabolism will influence the utilization efficiency of dietary amino acids and therefore their requirements. Furthermore, it is not known how inter-organ amino acid exchange and splanchnic uptake are affected under pathologic conditions, and how these processes influence amino acid requirements under conditions of disease. There are data on the splanchnic uptake of leucine, arginine, glutamine and methionine in healthy subjects. In the pediatric population there are two studies on leucine splanchnic uptake, in a population of patients with cystic fibrosis and in the newborn period. These studies yield leucine splanchnic uptake values of 24% and 30% respectively. There are no data on methionine and cysteine splanchnic uptake in healthy or sick children. Therefore, the first objective of this proposal is to determine the rates of splanchnic uptake of methionine and cysteine in sick children.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 斑点区域在整个体内氨基酸代谢的稳态中起着重要作用，并有选择地影响吸收的氨基酸的模式，后来释放到系统性循环中。在危害疾病期间，诊断区域受到深远的影响，可能会影响氨基酸代谢。关于器官间氨基酸交换的数据有限，氨基酸在重症患者中的诊断和分解代谢的作用有限。为了确定肠胃外和肠氨基酸的需求，有必要确定诊断区域氨基酸的第一次通过的程度。我们计划研究一群健康的青少年，他们将作为我们重病青少年组的控制。 尚未确定健康青少年中的硫氨基酸鼻子吸收，需要定义。因此，需要定义生病儿童中硫氨基酸的肠内需求。 该方案的第一个目的是在健康儿童青少年中对蛋氨酸和半胱氨酸诊断摄取的研究进行研究。对健康儿童的研究将作为我们重病人群的控制。我们要求GCRC支持在健康儿童中进行研究。 我们旨在在第一组研究中进行L- [1-13C]和L [2H2]甲硫氨酸的静脉静脉静脉内和肠内输注。 主要终点：蛋氨酸或半胱氨酸示踪剂的比例消失在通过闪烁的区域 该方案是我们对病儿童硫氨基酸代谢的研究的延续。我们已经对危害婴儿，儿童和青少年的蛋氨酸诊断摄取的研究进行了研究（请参阅初步数据）。 斑点区域在整个体内氨基酸代谢的稳态中起着重要作用。斑点区域选择性影响吸收的氨基酸的模式，后来释放到系统性循环中。肠道氨基酸代谢在调节吸收的饮食氨基酸进入门户循环中起着重要作用。因此，饮食中氨基酸的模式与门户静脉血液明显不同，并不能反映其对肠外组织的可用性。这个概念对蛋白质和氨基酸营养具有重要意义。小肠（体内大型器官）在第一次通过的饮食必需氨基酸的广泛分解代谢，导致营养效率降低。因此，肠道氨基酸代谢将影响饮食氨基酸的利用效率，因此会影响其需求。此外，尚不清楚在病理条件下如何影响器官间氨基酸的交换和闪烁体吸收，以及这些过程如何影响疾病条件下的氨基酸需求。 在健康受试者中，有有关亮氨酸，精氨酸，谷氨酰胺和蛋氨酸的斑点吸收的数据。在小儿人群中，有两项关于亮氨酸诊断摄取的研究，囊性纤维化患者和新生儿时期。这些研究产生的亮氨酸酶摄取值分别为24％和30％。健康或生病的儿童中没有关于蛋氨酸和半胱氨酸诊断摄取的数据。因此，该提案的第一个目的是确定生病儿童中蛋氨酸和半胱氨酸的诊断摄取率。