NUTRITIONAL AND FUNCTIONAL METHIONNE REQUIREMENTS IN SEPTIC ADOLESCENTS

脓毒症青少年的营养和功能性蛋硫氨酸需求

• 批准号: 7950611
• 负责人: Leticia Castillo
• 金额: $ 0.44万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7950611
• 关键词: Adolescent; Amino Acids; Anabolism; Child; Childhood; Choline; Clinical Research; Computer Retrieval of Information on Scientific Projects Database; Creatine; Critical Illness; Critically ill children; DNA Methylation; Data; Disease Marker; Equilibrium; Functional disorder; Funding; Goals; Gold; Grant; Homocysteine; Homocystine; Institution; Intake; Lymphocyte; Metabolism; Methionine; Newborn Infant; Nutritional; Nutritional Requirements; Outcome; Peripheral; Population; Production; Protein Biosynthesis; Research; Research Personnel; Resources; Severities; Source; Sulfur Amino Acids; Techniques; Time; United States National Institutes of Health; base; improved; oxidation; septic; sound

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This portocol is a continuation of our studies on sulfur amino acid metabolism in critically ill septic children. The immediate objectives of this project are to better define sulfur amino acid requirements in the healthy and in the critically ill septic pediatric population from newborn to adolescent. This is an ambitious goal that can only be approached with one project at a time. In this proposal we are focused exclusively in the healthy and critically ill adolescent population. Amino acid requirements in healthy children are based on extremely limited data. The amino acid requirements for critically ill children in therms of nutritional and functional balance are not known. Methionine, an indispensable sulfur amino acid serves for protein synthesis in addition to DNA methylation and biosynthesis of important compounds such as creatine, choline, etc. However, an excessive methionine administration may contribute to endothelial dysfunction of critical illness through an excessive production of homocysteine. In the present studies we propose to define nutritional requirements of methionine and to begin to explore functional requirements in 196 critically ill septic adolescents and 196 healthy adolescents over a period of 5 years. We propose to modify the "gold" standard techniqure of amino acid requirement determination, the 24h-indicator amino acid oxidation and balance technique, to make it applicable to both, the healthy and critically ill populations. We plan also to correlate methionine intake with severity of disease, markers of endothelial dysfunction and rates of DNA methylation in peripheral lymphocytes. Our long-term aim is to eventually determine the amino acid requirements that will maintain nutritional balance and function in the pediatric population from newborn to adolescents, to improve outcome in the sick population and to establish sound nutritional standards based on quantitative, reliable data in the healthy pediatric population.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该阶层是我们对危重的化脓性儿童中硫氨基酸代谢的研究的延续。 该项目的直接目标是更好地定义从新生儿到青少年的健康和败生小儿种群中的硫氨基酸的需求。 这是一个雄心勃勃的目标，一次只能与一个项目实现。 在此提案中，我们专注于健康且重病的青春期人群。 健康儿童中的氨基酸需求基于非常有限的数据。 未知营养和功能平衡热的重症儿童对重症儿童的氨基酸需求尚不清楚。 除了肌酸，胆碱等重要化合物的DNA甲基化和生物合成外，蛋氨酸是必不可少的硫氨基酸，可用于蛋白质合成。然而，过量的蛋氨酸给药可能导致过度的甲氨酸施用，从而导致严重疾病的内皮功能障碍。 在目前的研究中，我们建议定义蛋氨酸的营养需求，并开始在5年内探索196个重症败血性青少年和196个健康青少年的功能需求。 我们建议修改氨基酸需求确定的“金”标准技术，即24小时氨基酸氧化和平衡技术，以使其适用于健康和严重不良的人群。 我们还计划将蛋氨酸摄入与疾病的严重程度，内皮功能障碍的标志物以及周围淋巴细胞中DNA甲基化的速率相关。 我们的长期目的是最终确定从新生儿到青少年的小儿人群中维持营养平衡和功能的氨基酸需求，以改善病人的预后，并基于健康小儿人群的定量，可靠的数据来建立合理的营养标准。