Morphology Core Facility

形态学核心设施

• 批准号: 8118208
• 负责人: JAMES E DENNIS
• 金额: $ 6.6万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8118208
• 关键词: Area; Bioreactors; Cartilage; Collaborations; Core Facility; Data; Equipment; Freezing; Frozen Sections; Gadolinium; Glycosaminoglycans; Goals; Histologic; Histology; Image; Image Analysis; Immunochemistry; Individual; Internet; Laboratories; Location; Magnetic Resonance Imaging; Measurement; Methodology; Methods; Microscope; Microscopic; Microtomy; Morphology; Plastics; Preparation; Process; Qualifying; Research; Research Project Grants; Resources; Sampling; Schedule; Services; Specimen; Specimen Handling; Staining method; Stains; Technical Expertise; Techniques; Time; cost; experience; instrumentation; molecular imaging; programs; tissue culture

Morphology Core Facility All of the projects proposed in this Program Project rely on the use of morphological examinations and measurements as part of the analysis of their data. The goal of the Morphology Core is to provide the equipment and expertise required to process and deliver this high-quality histologic data as efficiently, and to provide expertise on the methods of sample preparation and analysis that is geared towards each particular research project. The Morphology Core is fully capable of processing specimens for a wide array of morphologic techniques including, rapid automated processing of standard histology samples, sectioning of those samples, preparation of samples tor frozen and plastic sections, immunochemistry, microscopic examination, image capture, and image analysis. The services to be provided by the Imaging Core are summarized by the following Specific Aims: Specific Aim 1: To provide a centralized processing area for the embedding, sectioning and staining of standard histologic samples. A well-equipped centralized histology laboratory with qualified technicians will prepare histologic samples using automated embedding equipment, high-end microtomy equipment for standard and frozen sections, with technical expertise, and standardized and immunohistochemical staining methodologies through the use of an experienced staff. Specific Aim 2: To provide a central laboratory for imaging and image analysis. Imaging facilities will center on a Leica upright research fluorescent and brightfield microscope and an Olympus inverted microscope for tissue culture observation plus a workstation for image analysis using Image Pro-Plus. Scheduling will be organized through the internet, as will image storage and downloading to individual laboratories. Expertise on imaging, image analysis and interpretation will be provided by the staff. Specific Aim 3: To apply non-invasive MRI for microstructral & molecular imaging of intact specimens. The Imaging Core will employ the resources of the Case Center for Imaging Research (CCIR) for routine analysis of specimens with MRI. These specialized and developing techniques include delayed Gadolinium Enhanced Magnetic Resonance Imaging of Cartilage (dGEMRIC) to assess glycosaminoglycan contents. These non-invasive methods can be applied to specimens at several time points without adversely disturbing the specimen, such as analysis of specimens in customized bioreactors. Expertise on MRI will be provided by the staff of the Imaging Core and the staff in collaboration with the staff of the CCIR. The advantages of having the Imaging Core are that the processing of samples can be conducted in one central location more cost-efficiently than in the individual labs and the centralization of the facilities allows all laboratories access to excellent equipment that can be maintained by an experienced staff. The extra advantage of this Imaging Core is the availability of experts in the field who have access to other professionals and imaging instrumentation on and off campus that may provide alternative approaches to the analysis of their specimens.

形态学核心设施 该计划项目中提出的所有项目都依赖于形态学检查和 测量作为对数据分析的一部分。形态学核心的目标是提供 设备和专业知识需要有效地处理和交付此高质量的组织学数据，并 提供针对每个特定的样品制备和分析方法的专业知识 研究项目。 形态核心完全能够处理各种形态学的标本 包括标准组织学样本的快速自动化处理，这些样本的分区，包括快速自动化处理， 制备样品冻结和塑料切片，免疫化学，微观检查，图像 捕获和图像分析。成像核心提供的服务由 遵循特定目的： 特定目的1：为嵌入，切片和染色提供集中处理区域 标准组织学样本。 设备齐全的集中组织学实验室，具有合格的技术人员将准备组织学 使用自动嵌入设备，标准和冷冻的高端微切设备的样品 通过技术专业知识以及标准化和免疫组织化学染色方法的部分 使用经验丰富的员工。 特定目的2：提供成像和图像分析的中央实验室。 成像设施将集中在左右直立的研究荧光和Brightfield显微镜上，以及 奥林巴斯倒置显微镜用于组织培养观察以及使用图像分析的工作站 Pro-Plus。时间表将通过互联网组织，以及图像存储和下载 个别实验室。工作人员将提供成像，图像分析和解释的专业知识。 具体目标3：应用非侵入性MRI进行完整标本的微观和分子成像。 成像核心将采用案例成像研究中心（CCIR）的资源 用MRI对标本的常规分析。这些专业和发展的技术包括延迟 dgeminage（DGemric）的gadolinium增强了磁共振成像，以评估糖胺聚糖 内容。这些非侵入性方法可以在几个时间点应用于标本，而不会不利 干扰样品，例如对定制生物反应器中标本的分析。 MRI的专业知识将是 由成像核心的工作人员和工作人员与CCIR的工作人员合作提供。 具有成像核心的优点是可以在 一个中心位置比单个实验室中的成本效益高，设施的集中化允许 所有实验室都可以使用经验丰富的员工来维护的优秀设备。额外 这种成像核心的优势是该领域的专家的可用性 以及校园内外的成像仪器，可能为分析提供其他方法 他们的标本。