Novel Optrode Devices for Neuroscientists: Packaging and Waveguide Solutions to M

面向神经科学家的新型光极器件：M 的封装和波导解决方案

• 批准号: 8058436
• 负责人: JOHN P SEYMOUR
• 金额: $ 17.56万
• 依托单位: NEURONEXUS TECHNOLOGIES
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-15 至 2013-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8058436
• 关键词: Animals; Automobile Driving; Behavior; Behavioral; Communities; Couples; Cues; Custom; Devices; Diffusion; Dimensions; Disease model; Excision; Face; Financial Support; Future; Hand; Ion Channel; Joints; Laboratories; Lesion; Light; Lighting; Measures; Methods; Modeling; Modification; Monitor; Morphologic artifacts; Mus; Neurons; Neurosciences; Neurosciences Research; Operative Surgical Procedures; Optics; Output; Pattern; Plague; Polymers; Population; Productivity; Protocols documentation; Research Personnel; Rodent; Science; Silicon Dioxide; Site; Solutions; Specificity; Surface Properties; System; Techniques; Technology; Tissues; Translating; United States National Institutes of Health; Work; base; cell type; cost; cost effective; cost effectiveness; density; design; improved; innovation; light intensity; meetings; neural circuit; novel; optical fiber; relating to nervous system; research study; response; tool

DESCRIPTION (provided by applicant): The objective of this application is to develop advanced optrode solutions for neuroscientists that fulfill the potential of optogenetic technology-achieving highly specific neural circuit control. Once developed, the neuroscientist's experimental options for optical stimulation will grow to two dimensions with no limits on the recording site placement. Our approach develops (i) practical, yet novel solutions for the packaging issues currently plaguing users, and (ii) custom waveguides capable of region-specific illumination with no electrical artifact, and is modularly integrated onto any existing NeuroNexus recording array. As an alternative to wafer- level integration, we have devised an approach that lowers cost by improving yield while increasing design options. This project will further optogenic techniques, which have shown excellent promise as tools that allow temporally precise, non-invasive control of activity in well- defined neuronal populations. This degree of control over neural firing allows specific monitoring of temporal activity patterns in the context of circuit dynamics, understanding changes due to plasticity, and responses to behavior and external cues, which is critically important for studying disease models as well. PUBLIC HEALTH RELEVANCE: The objective of this application is to develop advanced optrode solutions for neuroscientists that fulfill the potential of optogenetic technology-achieving highly specific neural circuit control. Once developed, the neuroscientist's experimental options for optical stimulation will grow to two dimensions with no limits on the recording site placement. Our approach develops (i) practical, yet novel solutions for the packaging issues currently plaguing users, and (ii) custom waveguides capable of region-specific illumination with no electrical artifact, and is modularly integrated onto any existing NeuroNexus recording array. As an alternative to wafer- level integration, we have devised an approach that lowers cost by improving yield while increasing design options. This project will further optogenic techniques, which have shown excellent promise as tools that allow temporally precise, non-invasive control of activity in well- defined neuronal populations. This degree of control over neural firing allows specific monitoring of temporal activity patterns in the context of circuit dynamics, understanding changes due to plasticity, and responses to behavior and external cues, which is critically important for studying disease models as well.

描述（由申请人提供）：本申请的目的是为神经科学家开发先进的光极解决方案，以实现光遗传学技术的潜力，实现高度特异性的神经回路控制。一旦开发出来，神经科学家的光学刺激实验选项将发展到二维，并且对记录位点的放置没有限制。我们的方法开发了（i）针对当前困扰用户的封装问题的实用而新颖的解决方案，以及（ii）能够实现区域​​特定照明而没有电伪影的定制波导，并且以模块化方式集成到任何现有的 NeuroNexus 记录阵列上。作为晶圆级集成的替代方案，我们设计了一种通过提高良率同时增加设计选项来降低成本的方法。该项目将进一步发展光原技术，该技术已显示出作为工具的良好前景，可以在明确的神经元群体中对活动进行时间精确、非侵入性控制。这种对神经放电的控制程度可以在电路动力学的背景下对时间活动模式进行特定的监测，了解可塑性引起的变化，以及对行为和外部线索的反应，这对于研究疾病模型也至关重要。 公共健康相关性：该应用的目的是为神经科学家开发先进的光极解决方案，以发挥光遗传学技术的潜力，实现高度特异性的神经回路控制。一旦开发出来，神经科学家的光学刺激实验选项将发展到二维，并且对记录位点的放置没有限制。我们的方法开发了（i）针对当前困扰用户的封装问题的实用而新颖的解决方案，以及（ii）能够实现区域​​特定照明而没有电伪影的定制波导，并且以模块化方式集成到任何现有的 NeuroNexus 记录阵列上。作为晶圆级集成的替代方案，我们设计了一种通过提高良率同时增加设计选项来降低成本的方法。该项目将进一步发展光原技术，该技术已显示出作为工具的良好前景，可以在明确的神经元群体中对活动进行时间精确、非侵入性控制。这种对神经放电的控制程度可以在电路动力学的背景下对时间活动模式进行特定的监测，了解可塑性引起的变化，以及对行为和外部线索的反应，这对于研究疾病模型也至关重要。