Role of the central nucleus of the amygdala during ethanol-rewarded instrumental tasks

杏仁核中央核在乙醇奖励的仪器任务中的作用

• 批准号: 10679383
• 负责人: Matilde Castro
• 金额: $ 4.77万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10679383
• 关键词: Affect; Alcohol consumption; Alcohols; Amygdaloid structure; Anatomy; Animal Behavior; Animals; Attention; Automobile Driving; Behavior; Behavioral; Cell Nucleus; Chronic; Communication; Consumption; Cues; Data; Dependence; Electrodes; Electrophysiology (science); Ethanol; Event; Food; Glass; Implanted Electrodes; Individual; Intake; Intervention; Intoxication; Learning; Mediating; Medical; Monitor; Motivation; Neurobiology; Neurons; Nutrient; Oral; Organism; Output; Pattern; Procedures; Process; Ramp; Rattus; Research; Research Personnel; Rewards; Role; Satiation; Science; Self Administration; Sodium; Substance Use Disorder; Techniques; Testing; Thirst; Time; Training; Translating; Volatilization; Water; Work; Writing; alcohol research; alcohol reward; alcohol seeking behavior; career; drinking; experience; experimental study; improved; in vivo; motivated behavior; motor behavior; neural; neural correlate; neurophysiology; novel therapeutics; optogenetics; pharmacologic; preference; programs; recruit; reinforcer; response; reward processing; skills

PROJECT SUMMARY The motivation to consume ethanol is a fickle thing. It differs wildly between individuals, but even in one individual, this motivation is not always constant. However, the exact dynamics that translate motivation to consume to the literal act of consumption remain poorly understood. The central nucleus of the amygdala may be an essential piece in this process. Its anatomical connections hint at its potential role in ethanol and food reward consumption, given that it is densely innervated by canonical reward processing centers and itself innervates regions directing motor behaviors and attention. However, the exact mechanisms and firing patterns that drive the central nucleus of the amygdala’s involvement in driving reward consumption remain poorly understood. This proposal makes use of a foundational technique, in vivo electrophysiology, to uncover how the central nucleus of the amygdala electrical activity relates to alcohol seeking and taking. In looking at the activity of this region over the course of a whole instrumental task session, a clearer conclusion about the central nucleus of the amygdala’s role in synthesizing motivation to drive motor behaviors can be obtained. In aim 1, the neural activity pattern in the central nucleus of the amygdala will be monitored over the course of an ethanol self-administration task to understand how activity in this region changes across trials as an animal’s motivation to consume ethanol wanes. For aim 2, the proposed electrophysiology recordings will occur when water-deprived rats perform a choice task that promotes a switch in reward preference from water to ethanol. These planned recordings will elucidate central nucleus of the amygdala activity when there are competing motivations. In aim 3, the effect of dependence on both instrumental tasks will be assessed to understand how the firing in this region following dependence-inducing high concentrations of ethanol. In completing these aims and disseminating the data I find, I will also further my independence and confidence as an independent researcher while also improving my theoretical, technical, and professional skills. Overall, this project aims to understand the central nucleus of the amygdala’s role as a nexus point between reward seeking and reward consumption. This would not only deepen our understanding of this nucleus’s role as a whole, but could potentially contribute to novel therapeutic behavioral and medical interventions.

项目摘要 食用乙醇的动机是一件好事。个人之间的差异很大，但即使是 个人，这种动机并不总是恒定的。但是，将动机转化为的确切动态 消费的文字消费行为仍然了解得很糟糕。杏仁核的中央核 在此过程中成为必不可少的部分。它的解剖联系暗示了其在乙醇和食物中的潜在作用 奖励消费，鉴于它被规范奖励处理中心和本身否认了 支配指导运动行为和注意力的地区。但是，确切的机制和射击模式 这推动了杏仁核参与推动奖励消费的中心核 理解。该建议利用基础技术，体内电生理学，以发现如何揭示 杏仁核电活动的中央核us与寻求酒精有关。看着 在整个乐器任务课程中，该地区的活动，关于 可以获得杏仁核在合成驱动运动行为动机中的作用的中央核。在 AIM 1，将监测杏仁核中央核中的神经活动模式。 乙醇自我管理任务，以了解该地区的活动在试验中如何变化 消耗乙醇减弱的动机。对于AIM 2，当提议的电生理记录将发生 贫困的大鼠执行了一项选择任务，可促进从水到乙醇的奖励偏好转换。 这些计划的记录将在竞争时阐明杏仁核活性的中央核 动机。在AIM 3中，将评估依赖对这两个工具任务的影响，以了解如何了解 依赖性诱导高浓度乙醇后，该区域的发射。完成这些目标 并传播我发现的数据，我还将进一步发展自己的独立性和信心 研究人员同时还提高了我的理论，技术和专业技能。总体而言，该项目的目的是 了解杏仁核角色的中心核作为寻求和奖励之间的联系 消耗。这不仅会加深我们对这个核整个角色的理解，而且可以 有可能导致新型的热行为和医疗干预措施。