Multimode Fluorescence Microplate Reader

多模式荧光酶标仪

• 批准号: 8052483
• 负责人: David Joseph Weber
• 金额: $ 16.44万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8052483
• 关键词: 10 year old; Baltimore; Basic Science; Biological Assay; Cancer Center; Collection; Enzyme Kinetics; Equipment; Fluorescence; Fluorescence Polarization; Fluorescence Resonance Energy Transfer; Funding; Lanthanoid Series Elements; Liquid substance; Luciferases; Maryland; Measurement; National Cancer Institute; Principal Investigator; Reader; Research; Research Personnel; Services; Speed; Time; United States National Institutes of Health; Universities; base; drug discovery; high throughput screening; instrument; luminescence; member; programs

DESCRIPTION (provided by applicant): NIH-funded principal investigators (PIs) at the University of Maryland, Baltimore (UMB) have a scientific need for a state-of-the-art multimode microplate reader to support and enhance their NIH funded research. This instrument will be located in and managed by the University of Maryland Marlene and Stewart Greenebaum Cancer Center (UMGCC) High Throughput Screening Shared Service (HTS SS) and will be available for members of the Cancer Center as well as for PIs throughout UMB. The microplate reader will replace the ~10 year old equipment currently in the HTS SS, and provide automated collection of absorbance, fluorescence intensity (FI), fluorescence polarization (FP), time resolve fluorescence (TR), TR-Fluorescence Resonance Energy Transfer (TR-FRET), luminescence, and AlphaScreen capabilities in 6 to 3456-well microplates. The existing microplate reader available in the HTS SS does not have auto-loading capabilities, is limited to 384- well format or less, lacks sensitivity in the FP mode, is not capable of lanthanide based TR-FRET assays or AlphaScreen assays, it does not have on-board fluid injectors for dual luciferase assays or rapid enzyme kinetic measurements, and lacks the overall speed and sensitivity needed by UMB investigators. This new multimode microplate reader will be central to the services provided by our HTS SS and will be critical to support basic science and drug discovery programs in the National Cancer Institute (NCI) designated UMGCC as well as for research of PI's throughout UMB who are funded by the NIH.

描述（由申请人提供）： 巴尔的摩马里兰大学 (UMB) 的 NIH 资助的主要研究人员 (PI) 对最先进的多模式酶标仪有科学需求，以支持和加强其 NIH 资助的研究。该仪器将位于马里兰大学 Marlene 和 Stewart Greenebaum 癌症中心 (UMGCC) 高通量筛查共享服务 (HTS SS) 并由其管理，并将可供癌症中心的成员以及整个 UMB 的 PI 使用。酶标仪将取代目前 HTS SS 中已有约 10 年历史的设备，并提供吸光度、荧光强度 (FI)、荧光偏振 (FP)、时间分辨荧光 (TR)、TR 荧光共振能量转移 (TR-荧光共振能量转移) 的自动收集（ TR-FRET）、发光和 AlphaScreen 功能，适用于 6 至 3456 孔微孔板。 HTS SS 中现有的酶标仪不具备自动加载功能，仅限于 384 孔格式或更小，在 FP 模式下缺乏灵敏度，不能进行基于镧系元素的 TR-FRET 测定或 AlphaScreen 测定。没有用于双荧光素酶测定或快速酶动力学测量的机载流体注射器，并且缺乏 UMB 研究人员所需的整体速度和灵敏度。这款新型多模式酶标仪将成为我们 HTS SS 提供的服务的核心，对于支持美国国家癌症研究所 (NCI) 指定的 UMGCC 的基础科学和药物发现项目以及整个 UMB 资助的 PI 的研究至关重要由美国国立卫生研究院。