Request for a Varian 600 MHz NMR Instrument

索取瓦里安 600 MHz NMR 仪器

• 批准号: 8052639
• 负责人: WILFRED A. VAN DER DONK
• 金额: $ 60万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8052639
• 关键词: Biological Factors; Biology; Blood capillaries; Flavonoids; Funding; Genomics; Goals; Housing; Illinois; Institutes; Methodology; Methods; Peptides; Plants; Request for Applications; Research; Research Personnel; Sampling; Solutions; Technology; United States National Institutes of Health; Universities; capillary; instrument; phosphonate

DESCRIPTION (provided by applicant): This application requests the purchase of a 600 MHz NMR spectrometer for multi-investigator use in the Institute for Genomic Biology (IGB) at the University of Illinois at Urbana-Champaign. The IGB houses a large number of NIH-funded research groups that are developing new methods for natural product discovery, a goal that is specifically noted in the NIH Roadmap. Researchers are developing new methodology to discover a wide range of natural products, from phosphonates and polyketides, to non-ribosomal peptides, plant-derived flavonoids, and ribosomally synthesized and post-translationally modified peptides. Current bottlenecks in these efforts are structural determination of mass-limited samples. Capillary LC NMR has been shown in recent years to provide a solution for such samples by requiring just 5-20 ¿L of sample and providing 10-fold increase in sensitivity over regular probes by using microcoil technology. This proposal describes the proposed use of CapLC NMR in combination with a 600 MHz NMR spectrometer. In this application funds are requested for a 600 MHz NMR spectrometer to house CapLC NMR probes that would be purchased by the IGB. Additional use by users at the IGB is also described. PUBLIC HEALTH RELEVANCE: This application requests funds to purchase a shared nuclear magnetic resonance instrument for use by eight different laboratories at the University of Illinois. The instrument will be used for the structure determination of a variety of natural products that have promise for human use as therapeutics, herbicides, or antifungal agents.

描述（由应用程序提供）：此应用程序要求购买600 MHz NMR光谱仪在伊利诺伊大学Urbana-Champaign大学的基因组生物学研究所（IGB）中使用600 MHz NMR光谱仪。 IGB拥有大量由NIH资助的研究小组，这些研究小组正在开发新的自然产品发现方法，这一目标是在NIH路线图中特别指出的。研究人员正在开发新的方法论，以发现各种天然产物，从磷酸盐和聚酮化合物到非核糖体肽，植物衍生的类黄酮以及核糖体合成和翻译后修饰的肽。在这些工作中，当前的瓶颈是质量受限样品的结构确定。近年来，毛细血管LC NMR已通过仅需5-20卢斯的样本，并通过使用微型机油技术来提供对常规问题的灵敏度提高10倍，从而为此类样品提供解决方案。该建议描述了CAPLC NMR与600 MHz NMR光谱仪的拟议使用。在此申请中，要求使用600 MHz NMR光谱仪来容纳IGB将购买的CAPLC NMR问题。还描述了用户在IGB上的其他用途。 公共卫生相关性：本申请要求资金购买共享的核磁共振工具，以供伊利诺伊大学的八个不同实验室使用。该仪器将用于结构确定各种自然产品，这些天然产品有望用作治疗，除草剂或抗真菌剂。

项目成果

18O Kinetic Isotope Effects Reveal an Associative Transition State for Phosphite Dehydrogenase Catalyzed Phosphoryl Transfer.

18O 动力学同位素效应揭示了亚磷酸脱氢酶催化磷酰基转移的缔合过渡态。

• DOI: 10.1021/jacs.8b06301
• 发表时间: 2018
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Howe,GraemeW;vanderDonk,WilfredA
• 通讯作者: vanderDonk,WilfredA

Ene Reductase Enabled Intramolecular β-C-H Functionalization of Substituted Cyclohexanones for Efficient Synthesis of Bridged Bicyclic Nitrogen Scaffolds.

Ene 还原酶使取代环己酮的分子内 β-C-H 功能化，以有效合成桥联双环氮支架。

• DOI: 10.1002/anie.202302125
• 发表时间: 2023
• 期刊: Angewandte Chemie (International ed. in English)
• 作者: Jiang,Guangde;Huang,Chunshuai;Harrison,Wesley;Li,Hongxiang;Zhou,Megan;Zhao,Huimin
• 通讯作者: Zhao,Huimin