4800 Plus MALDI TOF/TOF Analyzer

4800 Plus MALDI TOF/TOF 分析仪

• 批准号: 7794726
• 负责人: WILFRED A. VAN DER DONK
• 金额: $ 39.54万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-07 至 2011-07-06
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794726
• 关键词: Anabolism; Arts; Catalysis; Data; Dendrimers; Development; Drug Delivery Systems; Funding; Health; Human; Illinois; Investigation; Laboratories; Mass Spectrum Analysis; Nucleic Acids; Pathway interactions; Peptide Antibiotics; Polysaccharides; Request for Applications; Research Personnel; Sampling; Scientist; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Technology; Time; Universities; Weight; antimicrobial peptide; biomedical scientist; genome database; improved; instrument; metalloenzyme; molecular recognition; public health relevance; research study; sensor; volatile organic compound

DESCRIPTION (provided by applicant): This application requests the purchase of a Matrix-Assisted Laser Desorption Ionization (MALDI)-TOF-TOF (Time-of-Flight) mass spectrometry instrument for multi-investigator use in the Mass Spectrometry Laboratory (MSL) at the University of Illinois at Urbana- Champaign. The MSL serves a large number of departments at the University of Illinois and also receives many samples from outside. The requested instrument has greatly improved mass accuracy compared to our current MALDI instrument, and can deliver routine MS-MS data unlike our current instrument. Access to the requested state-of-the- art technology would enable many critical experiments in NIH-funded laboratories at the University of Illinois that cannot be performed at present. In particular, it will greatly aid the investigation of the biosynthesis of peptide antibiotics, the structural elucidation of cyclic antimicrobial peptides discovered from genome databases, the development of dendrimers as drug delivery vehicles, the development of important new sensors for volatile organic compounds, and the understanding of nucleic acid catalysis and molecular recognition. In addition, the requested instrument will be used to better understand metalloenzymes that are key to human health as well as to elucidate glycan biosynthetic pathways. PUBLIC HEALTH RELEVANCE: This application requests funds to purchase a state-of-the-art instrument that can accurately determine the weight of large biomolecules. In addition, the instrument can fragment the molecules and analyze the fragments, thereby providing further information that is key for biomedical scientists. The requested shared instrument will provide data for more than 100 scientists at the University of Illinois.

描述（由申请人提供）： 该应用程序要求购买矩阵辅助激光解吸电离（MALDI）-TOF-TOF（飞行时间）质谱仪器，用于在Urbana-Champaign的伊利诺伊大学伊利诺伊大学的质谱实验室（MSL）中使用多个评估器。 MSL为伊利诺伊大学的大量系服务，还从外部收到了许多样本。与我们当前的MALDI仪器相比，所请求的仪器的质量精度大大提高了，并且与当前的仪器不同，可以提供常规的MS-MS数据。访问所需的最先进的技术将使伊利诺伊大学NIH资助的实验室进行许多关键实验，目前无法进行。特别是，它将极大地帮助研究肽抗生素的生物合成，从基因组数据库中发现的环状抗微生物肽的结构性阐明，将树枝状聚合物作为药物递送车的发展，开发重要的新传感器的挥发性有机化合物的新传感器的发展，以及对核酸蛋白质酸蛋白质的理解。此外，请求的仪器将用于更好地了解人类健康和阐明聚糖生物合成途径的能醇。 公共卫生相关性：本申请要求资金购买可以准确确定大型生物分子重量的最先进工具。此外，该仪器可以分散分子并分析碎片，从而提供了进一步的信息，这是生物医学科学家的关键。所请求的共享工具将为伊利诺伊大学的100多名科学家提供数据。

项目成果

Structural determinants of macrocyclization in substrate-controlled lanthipeptide biosynthetic pathways.

• DOI: 10.1039/d0sc01651a
• 发表时间: 2020-06-25
• 期刊: Chemical science
• 影响因子: 8.4
• 作者: Bobeica SC;Zhu L;Acedo JZ;Tang W;van der Donk WA
• 通讯作者: van der Donk WA

Insights into methyltransferase specificity and bioactivity of derivatives of the antibiotic plantazolicin.

• DOI: 10.1021/cb501042a
• 发表时间: 2015-05-15
• 期刊: ACS chemical biology
• 影响因子: 4
• 作者: Hao Y;Saint-Vincent PMB;Sharma A;Mitchell DA;Nair SK
• 通讯作者: Nair SK

Peptide microarrays for the discovery of bioactive surfaces that guide cellular processes: a single step azide-alkyne "click" chemistry approach.

• DOI: 10.1039/c4tb00375f
• 发表时间: 2014-06
• 期刊: Journal of materials chemistry. B
• 作者: Douglas Zhang;K. Kilian

The Antimicrobial Activity of the Glycocin Sublancin Is Dependent on an Active Phosphoenolpyruvate-Sugar Phosphotransferase System.

• DOI: 10.1021/acsinfecdis.1c00157
• 发表时间: 2021-08-13
• 期刊: ACS infectious diseases
• 影响因子: 5.3
• 作者: Biswas S;Wu C;van der Donk WA
• 通讯作者: van der Donk WA

Improved production of class I lanthipeptides in Escherichia coli.

• DOI: 10.1039/d2sc06597e
• 发表时间: 2023-03-08
• 期刊: CHEMICAL SCIENCE
• 影响因子: 8.4
• 作者: Lee, Hyunji;Wu, Chunyu;Desormeaux, Emily K.;Sarksian, Raymond;van der Donk, Wilfred A.
• 通讯作者: van der Donk, Wilfred A.