Purchase of a Thermo Fisher Scientific LTQ Orbitrap XL Mass Spectrometer

购买 Thermo Fisher Scientific LTQ Orbitrap XL 质谱仪

基本信息

批准号：
7838622
负责人：
I JONATHAN AMSTER
金额：
$ 83.2万
依托单位：
UNIVERSITY OF GEORGIA
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-08-07 至 2012-08-06
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): This proposal requests funds to purchase a Thermo Fisher Scientific LTQ Orbitrap XL liquid chromatography-mass spectrometer for the University of Georgia. This instrument will be housed in the Proteomics and Mass Spectrometry (PAMS) Facility, which is a core facility that provides mass spectrometry service to the scientific community of the entire university. This instrument will provide liquid chromatography-tandem mass spectrometry (LC-MS/MS) capabilities that are not currently available in the PAMS facility, but that are required by a significant number of NIH-funded researchers at UGA. This instrument will support the research efforts of nine major users with fifteen NIH grants as well as 16 well-funded minor users, representing 11 different departments and four colleges across the university. The projects of the investigators include studies of human pathogens and parasites, insect vectors of human disease, fundamental investigations of the mechanisms of cancer, discovery of cancer biomarkers that can be used for early detection, neurochemistry, and basic studies of biology, all of which directly impact public health. The instrument will be used primarily for proteomic analysis, and to a lesser degree for lipidomic analysis. Protein analyses include identification, quantitation, and post-translational modification mapping. Capillary scale HPLC will be used on protein proteolytic digests, and analysis will include high resolution, accurate mass measurements and tandem mass spectrometry using collisionally induced dissociation and electron transfer dissociation. PUBLIC HEALTH RELEVANCE: The mass spectrometer that will be purchased with the funding of this proposal will provide analytical support for the research activities of nine major users who hold 15 NIH grants, as well as 16 minor users, most of whom are funded by NIH, or participate in NIH-funded research. The projects of the investigators include studies of human pathogens and parasites, insect vectors of human disease, fundamental investigations of the mechanisms of cancer, discovery of cancer biomarkers that can be used for early detection, and basic studies of biology, all of which directly impact public health. The funding of this proposal will impact job creation and maintenance in several tangible ways. First, the Vice President for Research of the University of Georgia will create and fund a position for a PhD level staff scientist in the Proteomics and Mass Spectrometry Facility (PAMS), should this proposal be funded (see attached letter of support). Furthermore, we anticipate that within a year or two, the income generated by this instrument will allow the PAMS facility to hire a technician. Thus, the funding of this grant will lead to the immediate creation of one job, and the future creation of a second job at the University of Georgia. The purchase of this mass spectrometer will impact jobs with the instrument vendor, as well. We have selected an instrument from Thermo Fisher Scientific, which is based in the United States, with headquarters in Waltham, Massachusetts. They employ approximately 20,000 people in more than 200 facilities across the country. Of these facilities, approximately 100 are used for manufacturing, including major sites in San Jose and Fremont, California; Asheville, North Carolina; Pittsburgh, Pennsylvania; Sugar Land, Texas; Madison, Wisconsin; Franklin, Massachusetts and Marietta, Ohio. About 80 percent of their US workforce is engaged in manufacturing, sales and service, engineering, and scientific research and development jobs. The environmental impact of the instrument is favorable. On the manufacturing side, Thermo Fisher Scientific is committed to being environmentally responsible in the way they run their company and in the products they manufacture for their customers. Their facilities worldwide operate under a program that generally follows the ISO 14001 environmental management standard, with additional requirements outlined in their internal energy and waste management policies. With regards to the environmental impact of the operation of the requested LC-mass spectrometer, we have specifically requested a capillary HPLC system with this instrument. This utilizes organic solvents at flow rates of a few ¿L/min, 1000 times lower than conventional liquid chromatography which uses mL/min flow rates. This ensures that this instrument will generate minimal (less than 5 mL per day) of chemical waste in its day-to-day operation.

描述（由申请人提供）：该提案请求资金为佐治亚大学购买 Thermo Fisher Scientific LTQ Orbitrap XL 液相色谱-质谱仪。该仪器将安装在蛋白质组学和质谱 (PAMS) 设施中。为整个大学的科学界提供质谱服务的核心设施该仪器将提供液相色谱-串联质谱（LC-MS/MS）。 PAMS 设施目前不具备这些功能，但 UGA 的大量 NIH 资助的研究人员需要这些功能。该仪器将支持拥有 15 项 NIH 拨款的 9 个主要用户以及 16 个资金充足的次要用户的研究工作。用户代表全校 11 个不同的院系和 4 个学院，研究人员的项目包括人类病原体和寄生虫、人类疾病的昆虫媒介的研究、癌症机制的基础研究、可用于早期诊断的癌症生物标志物的发现。检测、该仪器将主要用于蛋白质组学分析，并在较小程度上用于脂质组学分析，包括鉴定、定量和翻译后修饰毛细管分析。大规模高效液相色谱法将用于蛋白质水解消化，分析将包括高分辨率、精确的质量测量和使用碰撞诱导解离和电子转移解离的串联质谱分析。公共健康相关性：本提案资助购买的质谱仪将为拥有 15 项 NIH 资助的 9 个主要用户以及 16 个次要用户（其中大部分由 NIH 资助）的研究活动提供分析支持，或参与NIH资助的研究项目，包括人类病原体和寄生虫、人类疾病的昆虫媒介的研究、癌症机制的基础研究、发现可用于早期的癌症生物标志物。检测和生物学基础研究，所有这些都直接影响公共健康，该提案的资助将以多种具体方式影响就业机会的创造和维持。对于蛋白质组学和质谱设施（PAMS）的博士级科学家来说，如果该提案得到资助（请参阅随附的支持信），此外，我们预计在一两年内，该仪器产生的收入将允许PAMS 设施雇用一名技术员。这笔赠款的资助将导致立即创造一个就业机会，并且未来在佐治亚大学创造第二个就业机会。购买该质谱仪也将影响仪器供应商的就业机会。该公司总部位于美国马萨诸塞州沃尔瑟姆，在全国 200 多个工厂拥有约 20,000 名员工，其中约 100 个用于制造，其中包括位于美国的主要工厂。圣何塞加利福尼亚州的弗里蒙特、宾夕法尼亚州的匹兹堡、威斯康星州的麦迪逊、马萨诸塞州的富兰克林和俄亥俄州的玛丽埃塔。在制造方面，Thermo Fisher Scientific 致力于在其公司运营方式以及为客户生产的产品中对环境负责。根据通常遵循 ISO 的程序14001 环境管理标准，以及其内部能源和废物管理政策中概述的附加要求，关于所要求的 LC 质谱仪运行对环境的影响，我们特别要求配备该仪器的毛细管 HPLC 系统。溶剂的流速为几 ¿ L/min，比使用 mL/min 流速的传统液相色谱低 1000 倍，这确保了该仪器在日常操作中产生最少的化学废物（每天少于 5 mL）。

项目成果

期刊论文数量（39）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Heparin/heparan sulfate analysis by covalently modified reverse polarity capillary zone electrophoresis-mass spectrometry.

DOI：
10.1016/j.chroma.2018.02.052
发表时间：
2018-04-13
期刊：
Journal of chromatography. A
影响因子：
0
作者：
Sanderson P;Stickney M;Leach FE 3rd;Xia Q;Yu Y;Zhang F;Linhardt RJ;Amster IJ
通讯作者：
Amster IJ

Radical Cation Cyclopropanations via Chromium Photooxidative Catalysis.