Exploring Critical Components for MutS Activity in Helicobacter pylori

探索幽门螺杆菌 MutS 活性的关键成分

• 批准号: 8132560
• 负责人: ROBERT J. MAIER
• 金额: $ 22.05万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8132560
• 关键词: 8-hydroxyguanosine; Attention; Bacteria; Binding; Biological Assay; Complex; DNA; DNA Binding; DNA Binding Domain; DNA Repair; DNA Repair Endonuclease; DNA Structure; Defense Mechanisms; Enzymes; Family; Foundations; Funding; Gastric mucosa; Genetic; Genetic Recombination; Genome; Goals; Harvest; Health; Helicobacter; Helicobacter pylori; Homologous Gene; Host Defense; Human; Human Resources; Immunoprecipitation; In Vitro; Manuscripts; Molecular; Mutagenesis; Mutation; Oxidative Stress; Play; Procedures; Process; Proteins; Publishing; Research Project Grants; Resistance; Role; Site-Directed Mutagenesis; Stomach; Testing; Universities; Wages; Work; base; endonuclease; innovation; macromolecule; member; oxidative damage; pathogen; protein complex; protein crosslink; protein protein interaction; recombinational repair; repaired

DESCRIPTION (provided by applicant): Helicobacter pylori is a gastric pathogen of humans, and its persistence in the gastric mucosa has been in part attributed to its ability to counteract the host defense. The bacterium's marked ability to repair its oxidized macromolecules, including its DNA is one factor facilitating Helicobacter survival. The H. pylori MutS protein appears to play multiple and unique roles in DNA recognition, repair, and in recombination. However, the molecular mechanisms underlying the enzymes diverse functions are not understood, nor have its interacting partner proteins been identified. The goal here is to begin to understand the MutS repair function on a molecular level via enzyme assays, site-specific mutagenesis, and protein-protein interaction approaches. PUBLIC HEALTH RELEVANCE: The project involves characterization of factors used by a prevalent human pathogen to enable its survival in humans. The long term goal is to be able to thwart the ability of the bacterium to resist the defense mechanisms mounted by the host.

描述（由申请人提供）：幽门螺杆菌是人类的胃病原体，其在胃​​粘膜中的持久性部分归因于其能够抵消宿主防御的能力。该细菌可以修复其氧化的大分子（包括其DNA）的明显能力是促进旋律杆菌存活的一个因素。幽门螺杆菌MUTS蛋白似乎在DNA识别，修复和重组中起多种和独特的作用。然而，尚不清楚酶不同功能的分子机制，也没有鉴定出其相互作用的伴侣蛋白。这里的目的是开始通过酶测定，位点特异性诱变和蛋白质 - 蛋白质相互作用方法来理解分子水平的MUT修复功能。公共卫生相关性：该项目涉及对普遍的人类病原体使用的因素来表征其在人类中的生存。长期目标是能够阻止细菌抵抗宿主安装的防御机制的能力。

项目成果

Molecular basis for the functions of a bacterial MutS2 in DNA repair and recombination.

• DOI: 10.1016/j.dnarep.2017.07.004
• 发表时间: 2017-09
• 期刊: DNA repair
• 影响因子: 3.8
• 作者: Wang G;Maier RJ
• 通讯作者: Maier RJ

Helicobacter pylori peptidoglycan modifications confer lysozyme resistance and contribute to survival in the host.

幽门螺杆菌肽聚糖修饰赋予溶菌酶抗性并有助于在宿主中生存。

• DOI: 10.1128/mbio.00409-12
• 发表时间: 2012
• 期刊: mBio
• 影响因子: 6.4
• 作者: Wang,Ge;Lo,LejaF;Forsberg,LennartS;Maier,RobertJ
• 通讯作者: Maier,RobertJ