Repair of methionine residues in H. pylori catalase

幽门螺杆菌过氧化氢酶中蛋氨酸残基的修复

• 批准号: 7530074
• 负责人: ROBERT J. MAIER
• 金额: $ 7.38万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-11 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7530074
• 关键词: Address; Amino Acids; Back; Bacteria; Binding; Biochemical; Cells; Characteristics; Complement; Depth; Enzymes; Future; Goals; Grant; Helicobacter pylori; Individual; Inflammation; Knowledge; Methionine; Molecular; Monitor; Organism; Outcome; Oxidative Stress; Parents; Peptic Ulcer; Physiology; Procedures; Process; Proteins; Public Health; Publications; Purpose; Research; Research Project Grants; Resistance; Role; Site; Stomach; Stress; System; Thioredoxin; Work; base; benzyl-(1-amino-3-chloro-2-propanol); catalase; design; interest; macromolecule; malignant stomach neoplasm; methionine sulfoxide; methionine sulfoxide reductase; mutant; oxidation; pathogen; repair enzyme; repaired; therapeutic target

DESCRIPTION (provided by applicant): Methionine sulfoxide reductases (Msr's) catalyze the reduction of methionine sulfoxide back to (normal) methionine. The result is reactivation of oxidatively damaged proteins and this activity is known to facilitate successful gastric colonization by H. pylori. This focused project is designed to identify the critical amino acid residues associated with one identified H. pylori protein (catalase) that undergoes this reductive repair process. Identification of the specific Met residues repaired by Msr is of the most immediate interest. The residues will be identified by studying pure H. pylori Msr and its interaction with pure catalase. The information from the pure protein studies will be used to assess the degree of oxidative Met residue damage the target protein sustains upon stress agent exposure of whole cells. The proposed project is a small self-contained one on a highly successful pathogen. PUBLIC HEALTH RELEVANCE: The repair of an amino acid (methionine) by a bacterium that causes peptic ulcers and gastric cancers is important for the pathogens survival in the host. It is proposed to understand what factors influence the need for this repair in a key protein.

描述（由申请人提供）：甲硫氨酸还原酶（MSR）催化甲硫氨酸的硫氨酸还原回（正常）蛋氨酸。结果是氧化受损的蛋白质的重新激活，已知该活性促进幽门螺杆菌成功的胃定植。这个重点项目旨在识别与经历此还原性修复过程的鉴定出的幽门螺杆菌蛋白（过氧化氢酶）相关的关键氨基酸残基。 MSR修复的特定MET残留物的识别是最直接的利益。残基将通过研究纯H.幽门螺杆菌MSR及其与纯过氧化氢酶的相互作用来鉴定。来自纯蛋白质研究的信息将用于评估氧化损害的氧化程度，靶蛋白在全细胞的压力剂暴露时承受。拟议的项目是一个在非常成功的病原体上的小型独立项目。公共卫生相关性：通过引起消化性溃疡和胃癌的细菌修复氨基酸（蛋氨酸）对于宿主的病原体生存至关重要。建议了解哪些因素会影响关键蛋白质中这种修复的需求。