X-Ray Chrystallography

X射线晶体学

基本信息

批准号：
8084108
负责人：
John M Pascal
金额：
$ 7.29万
依托单位：
THOMAS JEFFERSON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-06-03 至 2013-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8084108
关键词：
Address Area Arrestins Arts Back Binding Biochemical Biochemistry Cancer Center Charge Clathrin Collaborations Communities Complex Consultations Core Facility Crystallization Crystallography DNA Data Data Collection Drops Dynein ATPase Equipment Experimental Designs FHIT gene Faculty Family Future Goals HIV Housing Hydra Polyps Improve Access Investigation Liquid substance Malignant Neoplasms Manuals Mass Spectrum Analysis Mission Molecular Molecular Biology Mothers Motor N-terminal Pattern Point Mutation Positioning Attribute Precipitation Process Production Protein Analysis Protein Fragment Proteins Proteolysis Proteomics RNA Research Research Personnel Resource Sharing Robot Roentgen Rays Sampling Screening procedure Services Solutions Structure System Technology Testing Therapeutic Therapeutic antibodies Time Universities Water Work X-Ray Crystallography base cost effective distilled alcoholic beverage experience gel electrophoresis improved indexing inhibitor/antagonist interest member novel professor programs protein aminoacid sequence research study small molecule structural biology

项目摘要

X-RAY CRYSTALLOGRAPHY SHARED RESOURCE The X-ray Crystallography shared resource provides KCC investigators the ability to obtain structural information directly relevant to their research interests. This includes the determination of de novo structures, structural consequences of point mutations, co-crystallization with small molecules including inhibitors and potential therapeutics, and multicomponent systems including proteins, DNA and/or RNA. Although structure determination is a highly specialized, labor-intensive endeavor, the information obtained from these investigations frequently addresses seemingly disparate biochemical data and invariably improves future research efforts. Significant progress has been made to improve access to the facility for the KCC community. For instance, a liquid handling robot now tests for crystallization feasibility with significantly less material and personal effort, making preliminary screening experiments cost effective. This has encouraged a number of KCC members to consider employing structural biology in their program. The Department of Biochemistry and Molecular Biology recognizes the significance of structural biology to the university, and has made an additional hire in structural biology at the assistant professor level. With the increase of users and the new hire, we anticipate that a number of new structures will be forthcoming, most with immediate relevance to molecular origin of cancer.

X 射线晶体学共享资源 X 射线晶体学共享资源为 KCC 研究人员提供了获得结构的能力与他们的研究兴趣直接相关的信息。这包括从头结构的测定，点突变的结构后果，与小分子（包括抑制剂）的共结晶潜在的治疗方法，以及包括蛋白质、DNA 和/或 RNA 在内的多组分系统。虽然结构测定是一项高度专业化、劳动密集型的工作，从这些过程中获得的信息调查经常涉及看似不同的生化数据，并且总是会改善未来研究工作。在改善 KCC 社区使用该设施的便利性方面已取得重大进展。例如，一个液体处理机器人现在可以用更少的材料和个人工作来测试结晶的可行性，使初步筛选实验具有成本效益。这鼓励了许多 KCC 成员考虑在他们的项目中采用结构生物学。生物化学与分子生物学系认识到结构生物学对大学的重要性，并额外聘用了结构生物学生物学助理教授级别。随着用户和新员工的增加，我们预计许多新结构即将出现，其中大多数与癌症的分子起源直接相关。