Imaging of Inflammation and Treatment: Basic and Translational Potential

炎症成像和治疗：基础和转化潜力

• 批准号: 8088215
• 负责人: XUEDING WANG
• 金额: $ 30.92万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-10 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8088215
• 关键词: Affect; Affinity; Animals; Antirheumatic Agents; Arthritis; Biocompatible; Blood; Blood Volume; California; Chemical Engineering; Clinical; Contrast Media; Development; Devices; Diagnostic Imaging; Disease; Disease model; Drug Delivery Systems; Drug Evaluation; Drug Kinetics; Elements; Engineering; Environment; Etanercept; Evaluation; FDA approved; Functional Imaging; Gold; Human; Hypoxia; Image; Imagery; Imaging technology; Incidence; Inflammation; Inflammatory; Joints; Label; Laboratory Animals; Laboratory Study; Lasers; Life; Light; Magnetic Resonance Imaging; Malignant Neoplasms; Medical; Medical Imaging; Medicine; Metabolic; Methods; Michigan; Modeling; Molecular; Molecular Target; Monitor; Nuclear; Optics; Pain; Pattern; Peripheral; Pharmaceutical Preparations; Pharmacologic Substance; Physiological; Play; Proteins; Radiology Specialty; Rattus; Research; Research Personnel; Resolution; Resources; Rheumatoid Arthritis; Rheumatology; Role; Route; Schools; Signal Transduction; Speed; Synovitis; System; Techniques; Technology; Testing; Therapeutic; Therapeutic Agents; Time; Tissues; Transducers; Treatment Efficacy; Tumor Necrosis Factor-alpha; Ultrasonic Transducer; Ultrasonography; United States National Institutes of Health; Universities; Work; absorption; base; clinical practice; cytokine; design; disease diagnosis; hemodynamics; imaging modality; improved; in vivo; innovation; instrument; instrumentation; interest; intravenous administration; medical schools; molecular imaging; nanocolloid; nanorod; novel; optical imaging; overexpression; polyvinylidene fluoride; response; soft tissue; subcutaneous; success; time use; tomography; tool; treatment effect; uptake

DESCRIPTION (provided by applicant): The therapy and diagnosis of diseases at the cellular and molecular levels will be improved greatly with the development of medical imaging technologies that can not only monitor the delivery of analytical probes or therapeutic agents but also enable comprehensive and objective evaluation of treatment effects. As an emerging noninvasive, nonionizing light-based technology presenting both high contrast and high spatial resolution, photoacoustic tomography (PAT) may be developed into a novel and promising molecular imaging modality which will generate innovations and play a critical role in medicine. We propose to investigate an even more advanced spectroscopic PAT (SPAT) technique using inflammatory arthritic disease models, patterned after widely known, very costly, painful, and disabling disorders, for demonstration of the method. SPAT, when aided by a newly developed gold nanocolloid contrast agent, may create innovations with an extensive impact in various biomedical applications, not only in more efficient evaluation of drug effects in living laboratory animals but also in optimized therapeutic decisions in clinical practice. The SPAT system for human/animal inflammatory arthritis will be developed on a stand-alone commercial ultrasound unit which, in an exclusive research mode allows acquisition of light-induced ultrasound signals with 64 independent channels simultaneously. This capability will for the first time enable near real-time 2D PAT and fast speed 3D PAT. Gold nanorods with the optical absorption peak tuned to the NIR region will be conjugated with Etanercept (Enbrel(r)), an FDA approved drug for rheumatoid arthritis. Due to the strong optical absorption by gold nanorods, the SPAT system working with a designed PVDF array transducer holds promise in imaging the drug uptake in regional arthritic tissues. Besides drug delivery monitoring, the unique ability of SPAT in evaluating treatment efficacy will also be examined through the comparison with nuclear imaging and MRI conducted on the same animals quasi-simultaneously. By imaging the morphological and physiological hallmarks of synovitis including hypervascularization and hypoxia, SPAT may realize comprehensive evaluation of the early responses of arthritic tissues to the pharmaceutical treatment. This research is anticipated to develop instrumentation and techniques that may significantly contribute to diagnostic imaging and therapeutic monitoring of various disorders including inflammatory diseases and cancer.

描述（由申请人提供）：随着医学成像技术的开发，将大大改善在细胞和分子水平上的疾病的治疗和诊断，这些技术不仅可以监测分析探针或治疗剂的交付，而且还可以对治疗效应进行全面和客观的评估。作为一种新兴的非侵入性，非离子光基技术，具有高对比度和高空间分辨率，光声断层扫描（PAT）可以发展为一种新颖而有希望的分子成像方式，该模式将产生创新并在医学中发挥关键作用。我们建议使用炎症性关节炎模型进行更先进的光谱PAT（SPAT）技术，该模型以广泛已知的，非常昂贵，痛苦和残疾的疾病为图案，以证明该方法。当新开发的金纳米胶体对比剂的帮助时，吐口水可能会产生对各种生物医学应用产生广泛影响的创新，这不仅在对生物实验室动物中药物影响的更有效评估中，而且在临床实践中优化的治疗决策中。人/动物炎症性关节炎的吐痰系统将在独立的商业超声单元上开发，该单元在独家研究模式下，可以同时获得具有64个独立通道的光诱导的超声信号。此功能将首次启用近实时2D PAT和快速速度3D PAT。具有调谐到NIR区域的光吸收峰的金纳米棒将与Etanercept（Enbrel（r））共轭，这是一种FDA批准的类风湿关节炎药物。由于金纳米棒具有强烈的光学吸收，因此使用设计的PVDF阵列传感器的吐痰系统在成像区域性关节组织中的药物吸收方面有望。除了药物输送监测外，还将通过与核成像进行比较和在同一动物上进行的核成像进行比较来检查吐痰在评估治疗疗效方面的独特能力。通过成像滑膜炎（包括高血管化和缺氧）的形态和生理标志，SPAT可以实现关节炎组织对药物治疗的早期反应的全面评估。预计这项研究将开发仪器和技术，这些技术可能会显着有助于对包括炎症性疾病和癌症在内的各种疾病的诊断成像和治疗监测。

项目成果

A fiber-optic system for dual-modality photoacoustic microscopy and confocal fluorescence microscopy using miniature components.

• DOI: 10.1016/j.pacs.2013.07.001
• 发表时间: 2013-05-01
• 期刊: PHOTOACOUSTICS
• 影响因子: 7.9
• 作者: Chen, Sung-Liang;Xie, Zhixing;Guo, L Jay;Wang, Xueding
• 通讯作者: Wang, Xueding

Evaluation of bladder microvasculature with high-resolution photoacoustic imaging.

• DOI: 10.1364/ol.36.004815
• 发表时间: 2011-12-15
• 期刊: Optics letters
• 影响因子: 3.6
• 作者: Xie Z;Roberts W;Carson P;Liu X;Tao C;Wang X
• 通讯作者: Wang X

Quantitatively Understanding Cellular Uptake of Gold Nanoparticles via Radioactivity Analysis.

通过放射性分析定量了解金纳米粒子的细胞摄取。

• DOI: 10.1166/jnn.2015.9486
• 发表时间: 2015
• 期刊: Journal of nanoscience and nanotechnology
• 作者: Shao,Xia;Schnau,Paul;Qian,Wei;Wang,Xueding
• 通讯作者: Wang,Xueding

Sonophoric nanoprobe aided pH measurement in vivo using photoacoustic spectroscopy.

• DOI: 10.1039/c3an00093a
• 发表时间: 2013-06-07
• 期刊: The Analyst
• 作者: Ray A;Yoon HK;Koo Lee YE;Kopelman R;Wang X
• 通讯作者: Wang X

Photoacoustic imaging with a commercial ultrasound system and a custom probe.

• DOI: 10.1016/j.ultrasmedbio.2010.12.005
• 发表时间: 2011-03
• 期刊: ULTRASOUND IN MEDICINE AND BIOLOGY
• 影响因子: 2.9
• 作者: Wang, Xueding;Fowlkes, J. Brian;Cannata, Jonathan M.;Hu, Changhong;Carson, Paul L.
• 通讯作者: Carson, Paul L.