DNA MICROARRAY AND EXPRESSION CORE

DNA 微阵列和表达核心

• 批准号: 8172943
• 负责人: Deepak Kaushal
• 金额: $ 6.18万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172943
• 关键词: Applications Grants; Biological Assay; Brain; Color; Communities; Computer Retrieval of Information on Scientific Projects Database; Custom; DNA Microarray Chip; Data; Data Analyses; Experimental Designs; Funding; Gene Silencing; Grant; Human; Individual; Institution; Lead; Macaca; Macaca mulatta; MicroRNAs; Mycobacterium tuberculosis; Pathogen detection; Publications; RNA; Research; Research Personnel; Resources; Sampling; Services; Small Interfering RNA; Source; Spottings; Standardization; Tissue Microarray; United States National Institutes of Health; Work; base; design; pathogen; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The DNA Microarray and Expression Core (Microarray Core) at TNPRC currently provides the following services: A. DNA Microarrays: 1) Microarray experimental design; 2) Spotted or 2-color microarray experiments; 3) Microarray Data Analysis; 4) Small Sample Amplification; 5) PCR Arrays; and 6) RNA Isolation. B. siRNA based gene-silencing: 1) Design and synthesis of a subset of siRNA's useful to the TNPRC community; 2) Optimization and standardization of individual siRNA assay. During the year 2009, the core performed approximately 150 microarray hybridizations for various investigators. In all 1.0 Terabytes of raw data was accumulated. In contrast to the prior year when the majority were M. tuberculosis arrays this year the majority of the work were host arrays, including rhesus macaque microarrays (64), human microarrays (12) and mammalian miRNA microarrays (20). Some of the data generated has already lead to publications (8 publications), while much more is currently being analyzed. One additional service was added to the core activities in the last year: miRNA array. This service has been very popular, and has already contributed to the submission of two grant applications, of which one has been successful, while the other remains pending. In the coming years, the core would like to expand into custom microarrays. The first few arrays that will be designed, fabricated and produced by the core will include rhesus macaque tissue arrays (Gut, brain), a pan-macaque lympho-chip and a multi-pathogen detection chip for surveillance of the "pathogen-free" macaque colony.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 TNPRC的DNA微阵列和表达核心（微阵列）当前提供以下服务：A。DNA微阵列：1）微阵列实验设计； 2）发现或2色微阵列实验； 3）微阵列数据分析； 4）小样品放大； 5）PCR阵列；和6）RNA隔离。 B.基于siRNA的基因沉默：1）设计和合成siRNA对TNPRC社区有用的子集； 2）单个siRNA分析的优化和标准化。 在2009年，核心对各种研究人员进行了大约150个微阵列杂交。 在所有1.0保持trabytes中，原始数据都累积了。与上一年的大多数是结核分枝杆菌阵列相比，大多数工作是宿主阵列，包括鼠尾草猕猴的微阵列（64），人类微阵列（12）和哺乳动物mirna微阵列（20）。产生的一些数据已经导致出版物（8个出版物），而目前正在分析更多数据。去年，核心活动添加了另外一项服务：mirna阵列。这项服务非常受欢迎，并且已经为提交两个赠款申请做出了贡献，其中一项是成功的，而另一个仍在待定。 在未来几年中，核心希望扩展到自定义微阵列。由核心设计，制造和生产的前几个阵列将包括猕猴的组织阵列（肠道，脑），泛麦克晶淋巴片和多病原原检测芯片，用于监视“无病原体”猕猴菌落。