FUNCTIONAL ORGANIZATION OF AUDITORY CORTEX

听觉皮层的功能组织

• 批准号: 8172420
• 负责人: TODD M PRUESS
• 金额: $ 5.48万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172420
• 关键词: Agglutinins; Animals; Antibodies; Area; Auditory area; Cells; Computer Retrieval of Information on Scientific Projects Database; Evolution; Funding; Glutamate Transporter; Grant; Histological Techniques; Histology; Human; Institution; Lateral Geniculate Body; Macaca; Medial; Monkeys; Pan Genus; Parvalbumins; Pattern; Reporting; Research; Research Personnel; Resources; Scanning; Slide; Source; Staining method; Stains; Techniques; Temporal Lobe; Tissue Banks; United States National Institutes of Health; Visual; Wisteria; comparative; neuroimaging

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project seeks to understand the organization of auditory cortex in humans and the evolution of human-specific features of auditory cortex by comparing humans with chimpanzees (the animals most closely related to humans) and in macaque monkeys. We used neuroimaging and histological techniques to define higher-order auditory areas in humans, identify homologous areas in chimpanzees and macaques, and determine patterns of connectivity between auditory areas in the three species. During the reporting period, we built a comparative tissue collection to document the histology of primary and secondary auditory areas in macaques and chimpanzees. This involved sectioning two temporal lobes for each species, staining sets of sections for each species for Nissl, parvalbumin, and Wisteria florabunda agglutinin (WFA), and then scanning whole sections using the Center's Aperio slide scanning facility. This tissue collection is accessible for remote study by our colleagues at Vanderbilt and Nathan Kline. In addition, we stained sections of visual and auditory cortex with antibodies for glutamate transporters (VGLUT1, VGLUT2), These revealed the distribution of terminals originating in cells from the lateral geniculate and medial geniculate. This makes it possible to study the organization of thalamocortical connections in species that cannot be studied with invasive tracing techniques, specifically chimpanzees and humans.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该项目旨在通过将人类与黑猩猩（与人类最紧密相关的动物）和猕猴中的人类和猕猴中的人类和猕猴中的比较，以了解人类中听觉皮层的组织以及听觉皮层的人类特异性特征的组织。 我们使用神经影像学和组织学技术来定义人类的高阶听觉区域，识别黑猩猩和猕猴的同源区域，并确定三个物种中听觉区域之间连通性的模式。 在报告期间，我们建立了一个比较的组织收集，以记录猕猴和黑猩猩的原发性和次要听觉区域的组织学。 这涉及为每种物种的两个颞叶分段，为NISSL，Parvalbumin和Wisteria florabunda凝集素（WFA）的每个物种的截面染色集，然后使用中心的Aperio Aperio slide扫描设施扫描整个部分。 我们的同事在Vanderbilt和Nathan Kline的远程研究中可以访问此组织收集。 此外，我们用抗谷氨酸转运蛋白的抗体（VGLUT1，VGLUT2）染色了视觉和听觉皮层的切片，这些抗体揭示了源自侧向和内侧遗传的细胞中末端的分布。 这使得可以研究无法通过侵入性追踪技术（特别是黑猩猩和人类）研究的物种中丘脑皮质连接的组织。