PROMOTER-BASED FUNCTIONAL MAPPING OF AMYGDALA MICROCIRCUITS

基于启动子的杏仁核微电路功能图谱

• 批准号: 8172377
• 负责人: DONALD G RAINNIE
• 金额: $ 4.39万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172377
• 关键词: Amygdaloid structure; Cholecystokinin; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Green Fluorescent Proteins; Institution; Interneurons; Investigation; Maps; Neurons; Physiological; Property; Rattus; Reporting; Research; Research Personnel; Resources; Source; United States National Institutes of Health; base; in vivo; promoter

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During the reporting period we completed our investigation of the physiological and morphological properties of cholecystokinin (CCK) containing interneurons using the CCK promoter tagged with a green fluorescent protein (GFP). The resulting lentiviral construct was then used to transfect neurons in vivo, only those neurons that normally express CCK co-expressed GFP. We have used this approach to examine the physiological properties of CCK interneurons in the rat basolateral amygdala.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 在报告期间，我们使用用绿色荧光蛋白（GFP）标记的CCK启动子完成了含胆囊基蛋白（CCK）的生理和形态特性的研究。 然后，将所得的慢病毒构建体用于体内转染神经元，只有那些通常表达CCK共表达GFP的神经元。 我们已经使用了这种方法来检查大鼠基底外侧杏仁核中CCK中间神经元的生理特性。