A LIMBIC CIRCUIT ANALYSIS OF DEEP BRAIN STIMULATION FOR DEPRESSION

大脑深部刺激治疗抑郁症的边缘环路分析

• 批准号: 8357555
• 负责人: DONALD G RAINNIE
• 金额: $ 4.12万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357555
• 关键词: Acceleration; Animals; Behavior; Behavioral; Data; Deep Brain Stimulation; Electric Stimulation; Emotional; Emotions; Extinction (Psychology); Frequencies; Fright; Funding; Grant; Human; Learning; Manuscripts; Mental Depression; Modeling; National Center for Research Resources; Pattern; Preparation; Primates; Principal Investigator; Protocols documentation; Rattus; Research; Research Infrastructure; Resources; Source; Training; United States National Institutes of Health; Work; conditioned fear; cost; food restriction; learning extinction; relating to nervous system; research study; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In 2010, we continued work on Aim 1: Characterize spontaneous activity in the BLA and PFC, and evaluate the presence of synchronous oscillations. We have collected data from six rats undergoing fear conditioning and extinction training, and have begun work on a manuscript describing our findings. We have demonstrated that delta (1.5-4Hz) and gamma (30-80Hz) frequency bands in the local field potentials of the BLA and mPFC show unique patterns of increased coherence during fear learning and expression, and that these patterns are not seen in rats that do not learn the fear association, indicating a functional relationship with the emotional state of the animal. We have now begun experiments related to Aim 2: Evaluate neural and behavioral response to PFC stimulation. We have applied electrical stimulation modeled after human DBS parameters to the mPFC region thought to be homologous to Cg25, while recording behavior and neural responses in the BLA. By stimulating during the fear extinction training protocol, we hope to demonstrate a suppression of negative emotion and an acceleration of extinction learning. The rats being used in this experiment have just arrived and are being weighed daily in preparation for food restriction, the first step in the protocol.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的首席研究员可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 2010 年，我们继续致力于目标 1：表征 BLA 和 PFC 中的自发活动，并评估同步振荡的存在。 我们收集了六只接受恐惧调节和消退训练的老鼠的数据，并开始撰写描述我们发现的手稿。 我们已经证明，BLA 和 mPFC 局部场电位中的 delta (1.5-4Hz) 和 gamma (30-80Hz) 频带在恐惧学习和表达过程中表现出增加一致性的独特模式，并且这些模式在大鼠中没有看到不学习恐惧关联，表明与动物情绪状态的功能关系。 我们现在已经开始与目标 2 相关的实验：评估对 PFC 刺激的神经和行为反应。 我们将根据人类 DBS 参数建模的电刺激应用于被认为与 Cg25 同源的 mPFC 区域，同时记录 BLA 中的行为和神经反应。 通过在恐惧消退训练方案中进行刺激，我们希望展示对负面情绪的抑制和消退学习的加速。 本实验中使用的老鼠刚刚到达，每天都会称重，为食物限制做准备，这是该方案的第一步。