2/2-Atlanta Center for Translational Research in Endometriosis (ACTRE)

2/2-亚特兰大子宫内膜异位症转化研究中心 (ACTRE)

• 批准号: 8128699
• 负责人: WINSTON E THOMPSON
• 金额: $ 27.2万
• 依托单位: MOREHOUSE SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-15 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8128699
• 关键词: Adenoviruses; Affect; Apoptosis; Biological; Biomedical Research; Biopsy; Cell Culture Techniques; Cell Cycle; Cell model; Cells; Center for Translational Science Activities; Clinic; Clinical; Clinical Research; Clinical Sciences; Clinical Trials; Development; Disease; Doctor of Philosophy; Education; Endometrial; Estrogens; Family; Foundations; Future; Gel; Goals; Growth; Hormonal; Human; In Situ; Infertility; Institutes; Instruction; Investigation; Knowledge; Laboratories; Laboratory Research; Lead; Lesion; Mediator of activation protein; Mentors; Mentorship; Messenger RNA; Methods; Mitochondria; Modeling; Molecular; NF-kappa B; National Institute of Child Health and Human Development; Pain; Pathway interactions; Phenotype; Primary Cell Cultures; Principal Investigator; Productivity; Protein Family; Rattus; Regulation; Reporting; Reproduction; Research; Research Infrastructure; Research Personnel; Resistance; Science; Site; Specimen; Staging; Stromal Cells; Students; Symptoms; Techniques; Technology Transfer; Testing; Therapeutic Agents; Tissues; Training; Training Programs; Translational Research; Underrepresented Minority; United States National Institutes of Health; Universities; Woman; bench to bedside; cancer cell; career; design; drug development; efficacy testing; endometriosis; experience; in vivo; knock-down; medical schools; multidisciplinary; novel; novel therapeutics; overexpression; pre-clinical; programs; prohibitin; protein expression; public health relevance; reproductive; research study; response

DESCRIPTION (provided by applicant): We propose a new U01 Cooperative Research Partnership to Promote Workforce Diversity in the Reproductive Sciences in response to RFA-HD-09-008. The Atlanta Center for Translational Research in Endometriosis (ACTRE, pronounced "actor") is a partnership between the reproductive research programs of Emory University (EU) and Morehouse Schools of Medicine (MSM) and will be jointly directed by Robert N. Taylor, MD, PhD of EU and Winston E. Thompson, PhD of MSM. The leaders are highly respected independent investigators with extensive experience and productivity in coordinated, multidisciplinary reproduction research and education teams. Moreover, the program will be facilitated by extant infrastructure provided under the aegis of the Atlanta Clinical and Translational Science Institute, which has been jointly operated between EU and MSM since 2007. The NICHD theme of "cells to selves" (NIH Guide, January 30, 2002) serves as the biological foundation to study the fundamental mechanisms of endometriosis lesion growth and apoptosis and their relationship to pain and infertility. The scientific focus of ACTRE will concentrate on the regulation of endometriotic cell cycle dynamics to determine why endometriotic cell apoptosis is dysregulated and how might it be therapeutically induced. Aim #1 will identify specific survival factor pathways in tissue specimens from women with and without endometriosis. In particular, the NF-?B and prohibitin pathways will be emphasized. Primary cell cultures will be developed using well characterized methods pioneered by Dr. Taylor. In Aim #2, Dr. Thompson will use these cultures to test the effects of hormonal and nonhormonal apoptosis-inducing agents and validate specific cytoplasmic and mitochondrial mechanisms of apoptosis using mRNA profiling and 2D-gel phosphoproteomic techniques. Aim #3 will utilize rats bearing endometriosis-like lesions to test the efficacy of novel therapeutic agents designed to induce apoptosis in the lesions in vivo. The Scholars will be introduced in the clinic to women suffering from endometriosis and follow their endometrial specimens to the laboratory, where investigation of molecular mechanisms will take place. A diverse cadre of undergraduates will be exposed to the entire translational experience, from disease to possible drug development. Mentors from EU and MSM will guide scholars in clinical and laboratory research, didactic instruction, and provide individualized mentorship with the goal of facilitating matriculation in graduate and professional programs related to reproductive sciences. PUBLIC HEALTH RELEVANCE: The specific scientific theme of endometriotic lesion survival and resistance to apoptosis provides a targeted focus to engage underrepresented minority students about the intellectual excitement of reproductive science investigation and the unmet clinical needs of women suffering from this condition. Additionally, ACTRE will promote collaborative investigation into the causes and possible treatments of endometriosis across the greater Atlanta biomedical research consortium.

描述（由申请人提供）：我们提出了一种新的U01合作研究合作伙伴关系，以促进生殖科学中的劳动力多样性，以响应RFA-HD-09-008。亚特兰大子宫内膜异位症转化研究中心（ACTRE，发音为“演员”）是埃默里大学（EU）生殖研究计划与莫尔豪斯医学院（MSM）之间的合作伙伴关系，将由Robert N. Taylor，MD，EU和Winston E. Thompson E. Thompson E. Thompson，Phd of MSM的Robert N. Taylor，MD。领导者是高度尊敬的独立研究人员，在协调的多学科繁殖研究和教育团队中拥有丰富的经验和生产力。此外，该计划将由亚特兰大临床和转化科学研究所的宙斯盾下提供的现有基础设施促进，该研究所自2007年以来一直在欧盟和MSM之间共同运行。“牢房至自我”的NICHD主题（NIH指南，2002年1月30日，2002年1月30日）是研究其基础的基础，并为他们的基础机械和AP的基础提供了基础。不育。 ACTRE的科学重点将集中于子宫内膜细胞周期动力学的调节，以确定子宫内膜细胞细胞凋亡的为什么失调以及如何通过治疗诱导它。 AIM＃1将确定来自有或没有子宫内膜异位症的女性组织标本中的特定生存因子途径。特别是，将强调NF-？B和禁止途径。原代细胞培养物将使用泰勒博士开创的特征性良好的方法开发。在AIM＃2中，汤普森博士将使用这些培养物来测试激素和非激素凋亡诱导剂的影响，并使用mRNA分析和2D-Gel磷酸蛋白酶学技术验证凋亡的特定细胞质和线粒体机制。 AIM＃3将利用带有子宫内膜异位症病变的大鼠测试旨在诱导体内病变凋亡的新型治疗剂的功效。学者将在诊所中引入患有子宫内膜异位症的妇女，并遵循其子宫内膜标本到实验室，并在其中进行分子机制的研究。从疾病到可能的药物开发，各种各样的本科生将暴露于整个翻译经验。欧盟和MSM的导师将指导学者进行临床和实验室研究，教学教学，并提供个性化的指导，以促进与生殖科学有关的研究生和专业计划的入学。 公共卫生相关性：子宫内膜病变生存和对凋亡的抵抗的特定科学主题为吸引代表性不足的少数族裔学生的智力兴奋和遭受这种疾病遭受这种疾病的女性的临床需求的智力兴奋提供了针对性的重点。此外，ACTRE将促进对大亚特兰大生物医学研究联盟的子宫内膜异位症的原因和可能治疗的合作研究。