Photoacoustic imaging of functional domains on primary motor cortex of monkeys

猴子初级运动皮层功能域的光声成像

• 批准号: 8011214
• 负责人: Xinmai Yang
• 金额: $ 13.96万
• 依托单位: UNIVERSITY OF KANSAS LAWRENCE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8011214
• 关键词: Abbreviations; Animals; Area; Auditory; Blood; Brain; Brain imaging; Cerebrovascular Circulation; Core-Binding Factor; Craniotomy; DAQ; Detection; Development; Dura Mater; Environment; Extinction (Psychology); Forelimb; Functional Imaging; Functional Magnetic Resonance Imaging; Goals; Hemoglobin; Human; Image; Imagery; Imaging Techniques; Imaging technology; Lasers; Light; Macaca mulatta; Magnetic Resonance Imaging; Measures; Methods; Microscopy; Monitor; Monkeys; Motor Cortex; Movement; Noise; Optical Coherence Tomography; Optics; Parkinson Disease; Penetration; Physiologic pulse; Positron-Emission Tomography; Precentral gyrus; Primates; Procedures; Process; Property; Qualifying; Research; Resolution; Sampling; Signal Transduction; Solutions; Spin Labels; Stress; Stroke; Structure; Surface; System; Techniques; Temperature; Testing; Time; Tissue Sample; Tissues; Ultrasonic Transducer; Ultrasonics; Ultrasonography; Variant; Visual; absorption; attenuation; blood oxygen level dependent; cranium; data acquisition; diffuse optical tomography; imaging modality; irradiation; light scattering; nonhuman primate; optical imaging; public health relevance; relating to nervous system; soft tissue; somatosensory; success; tool

DESCRIPTION (provided by applicant): Functional detection in primate brains has particular advantages because of the similarity between non-human primate brain and human brain and the potential for relevance to a wide range of conditions such as stroke and Parkinson's disease. In the proposed research, we use photoacoustic imaging (PAI) to detect functional changes at primary motor cortex of rhesus monkeys. In a rhesus monkey, primary motor cortex is buried in the precentral gyrus, which extends down 8-9 mm below the surface. Therefore, the advantage of PAI for imaging deeper structures is particularly beneficial for this application. In summary, the advantages of utilizing PAI for the detection of primate brain function include: (1) durotomy is not required, therefore, it is a minimal-invasive process; (2) detecting optical absorption properties, which is intrinsically sensitive and provide high contrast; (3) imaging buried cortical structures; (4) high-resolution of ~100 5m; (5) potentially PAI can be performed in real-time. The long term goal of this research is to develop PAI as a qualified tool for high-resolution functional brain imaging in primate research. The objective of current research is to test the feasibility of PAI for detection of primate brain activation using a minimal-invasive procedure. The establishment of a minimal-invasive procedure for research is important because it reduces the stress on the animal while studies can be carried out in a friendly environment to produce highly reliable results. The underlying hypothesis of this proposal is that PAI can reliably detect primary motor cortex activation associated with forelimb movement in rhesus macaques through the dura matter, and provide depth-resolved functional information. The specific aims of this study include (1) to construct a PAI system for monkey brain cortex imaging; (2) to produce both structural and functional images on monkey brain cortex; (3) to compare the sensitivity and resolution of PAI with other established techniques. PUBLIC HEALTH RELEVANCE: In this project, we propose to test the feasibility of photoacoustic imaging technique for brain function imaging in non-human primates. With photoacoustic imaging, buried cortical structures might be imaged with optical contrast at ultrasound resolution. The success of this project will potentially contribute to studies involving stroke and Parkinson's disease in non- human primates.

描述（由申请人提供）：灵长类动物大脑中的功能检测具有特殊的优势，因为非人类灵长类动物的大脑和人脑之间的相似性以及与诸如中风和帕金森氏病等广泛疾病相关的潜力。 在拟议的研究中，我们使用光声成像（PAI）来检测恒河猴原发性运动皮层的功能变化。 在恒河猴中，将原发性运动皮质埋在前中央回中，该回中心延伸到表面以下8-9毫米处。 因此，PAI对更深层的结构的成像优势对于此应用尤其有益。 总而言之，利用PAI检测灵长类动物脑功能的优势包括：（1）不需要尿道切开术，因此，这是一个最小的侵入性过程； （2）检测具有本质上敏感并提供高对比度的光吸收特性； （3）成像掩埋的皮质结构； （4）〜100 5m的高分辨率； （5）可能实时执行PAI。 这项研究的长期目标是开发PAI作为灵长类动物研究中高分辨率功能性大脑成像的合格工具。 当前研究的目的是使用最小的侵入性程序测试PAI检测灵长类动物脑激活的可行性。 建立最少的研究侵入性程序很重要，因为它可以减少对动物的压力，同时可以在友好的环境中进行研究以产生高度可靠的结果。 该提案的基本假设是，PAI可以可靠地检测到与恒河猕猴中的前肢运动相关的原发性运动皮层激活，并提供深度分辨的功能信息。 这项研究的具体目的包括（1）构建用于猴子脑皮层成像的PAI系统； （2）在猴子脑皮层上同时产生结构图像； （3）将PAI的灵敏度和分辨率与其他既定技术进行比较。 公共卫生相关性：在这个项目中，我们建议测试非人类灵长类动物中脑功能成像的光声成像技术的可行性。 使用光声成像，在超声分辨率下，掩埋的皮质结构可能会通过光学对比度成像。 该项目的成功将有可能有助于涉及非人类灵长类动物的中风和帕金森氏病的研究。