Laser Scanning Confocal Microscope

激光扫描共焦显微镜

• 批准号: 7794252
• 负责人: Donna M Fekete
• 金额: $ 49.96万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-06 至 2011-05-05
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794252
• 关键词: Alphavirus; Arts; Axonal Transport; Biological Process; Biological Sciences; Biomedical Research; Blindness; Cell Lineage; Cellular biology; Complement; Cytoskeleton; Defect; Development; Diarrhea; Ear; Eye; Flavivirus; Funding; Gap Junctions; Genes; Growth; Growth Cones; Human; Image; Investments; Lasers; Life; Maintenance; Malignant neoplasm of pancreas; Methods; Microscope; Mitochondria; Nervous system structure; Neurodegenerative Disorders; Pain; Perception; Photoreceptors; Productivity; Purdue Cancer Center; Regulation; Reporting; Research; Research Infrastructure; Research Personnel; Salmonella; Sampling; Scanning; Sensory; TRP channel; Training; Universities; cancer initiation; career; cell motility; cellular imaging; deafness; imaging modality; instrumentation; neuronal growth; pathogen; repaired; sound; success; tool

DESCRIPTION (provided by applicant): Nineteen researchers in the Department of Biological Sciences and the Purdue Cancer Center at Purdue University seek support for advanced live cell imaging with a Zeiss LSM 710 confocal microscope on an inverted microscope platform equipped with an incubation chamber. Live cell imaging is at the forefront modern cell biology and lack of access to these methods cuts off Purdue investigators from key advances. Support for this microscope provides essential research infrastructure to a successful cadre of NIH-funded researchers with a sound record of productivity, reporting significant advances in biomedical research. Acquisition of this microscope by this user group will open new research avenues for proven researchers who are poised to move strongly and successfully into advanced live cell imaging using the LSM 710. A sampling of the NIH-funded research that purchase of this microscope will support includes: the development of the vertebrate eye and ear in an effort to discover new genes underlying blindness (Leung) or deafness (Fekete) in humans; the mechanism by which defects in axonal transport of mitochondria give rise to neurodegenerative disease (Hollenbeck); cell lineages involved in pancreatic cancer initiation (Konieczny); cellular details of replication and assembly of human pathogen alphaviruses and flaviviruses (Kuhn); the function of TRP channels that are important in a vast array of biological functions, including sensory perception, especially pain (Pak); development and maintenance of healthy photoreceptors (Chang, Ready), regulation of neuronal growth cone motility and guidance critical to nervous system growth and repair (Suter); how Salmonella exploits the host acting cytoskeleton network to promote Salmonella entry and induce diarrhea in humans (Zhou). The requested inverted microscope complements the recent acquisition of an upright Zeiss LSM 710 by the Purdue Life Sciences Fluorescent Imaging Facility. Having both platforms will allow Purdue investigators to move seamlessly between upright and inverted microscopes so as to choose the best tool for each enquiry. This will also provide economies in training, administration and support. The microscope will be a nexus of interdisciplinary exchange of ideas and methods. In addition to serving established investigators, it will provide a cadre of outstanding young researcher's state of the art instrumentation at the critical outset of their careers. Lack of advanced live cell imaging methods is a significant research bottleneck at Purdue and investment in this microscope will stimulate experimental and intellectual success in biomedical research.

描述（由申请人提供）：生物科学系的19位研究人员和普渡大学的普渡大学癌症中心寻求支持配备有饲养室的倒置显微镜平台上的Zeiss LSM 710共聚焦显微镜对先进的活细胞成像的支持。实时细胞成像是现代细胞生物学的最前沿，无法从关键进展中切断普渡大学研究人员的使用。对该显微镜的支持为成功的NIH资助研究人员的干部提供了重要的研究基础设施，并具有出色的生产力记录，并报告了生物医学研究的重大进展。 Acquisition of this microscope by this user group will open new research avenues for proven researchers who are poised to move strongly and successfully into advanced live cell imaging using the LSM 710. A sampling of the NIH-funded research that purchase of this microscope will support includes: the development of the vertebrate eye and ear in an effort to discover new genes underlying blindness (Leung) or deafness (Fekete) in humans;线粒体轴突运输缺陷的机制引起神经退行性疾病（Hollenbeck）；参与胰腺癌启动的细胞谱系（Konieczny）；人病病毒和黄病毒的复制和组装的细胞细节（Kuhn）； TRP通道在各种生物学功能中都很重要的TRP通道的功能，包括感觉知觉，尤其是疼痛（PAK）；健康光感受器的开发和维护（Chang，Ready），神经元生长锥运动的调节以及对神经系统生长和修复至关重要的指导（SUTER）；沙门氏菌如何利用宿主作用细胞骨架网络来促进沙门氏菌进入并诱导人类（Zhou）的腹泻。 所需的倒置显微镜补充了Purdue Life Sciences荧光成像设施对直立的Zeiss LSM 710的收购。拥有两个平台将使普渡大学的调查人员能够在直立和倒置的显微镜之间无缝移动，从而为每个查询选择最佳工具。这还将提供培训，管理和支持的经济体。显微镜将是跨学科交换思想和方法的联系。除了为既定的调查人员服务外，它还将在其职业生涯的关键开始时提供一批杰出的年轻研究人员的最佳研究人员。缺乏先进的活细胞成像方法是普渡大学（Purdue）的一项重要研究瓶颈，对该显微镜的投资将刺激生物医学研究中的实验和智力成功。