Acquisition of a Multiphoton Microscope for Cellular Programming

获取用于细胞编程的多光子显微镜

• 批准号: 7793841
• 负责人: DAVID F MEANEY
• 金额: $ 50万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793841
• 关键词: 12 year old; Applied Research; Area; Arts; Biomedical Engineering; Cartilage; Cell Culture Techniques; Cells; Complex; Dental; Disease; Drug Formulations; Engineering; Etiology; Hippocampus (Brain); Injury; Life; Messenger RNA; Microscope; Natural regeneration; Optics; Organism; Pennsylvania; Pharmacology; Phenotype; Population; Regenerative Medicine; Research; Schools; Science; System; Techniques; Technology; Therapeutic; Tissues; Universities; Work; base; in vivo; instrumentation; medical schools; meetings; nervous system disorder; neural circuit; neurobehavior; new technology; novel; programs

DESCRIPTION (provided by applicant): This shared instrumentation proposal for a customized multiphoton microscope is part of a larger institutional effort at Penn in cellular programming. Strategically, this effort lies at the intersection between five of Penn's schools - Medicine, Dental, Veterinary, Arts and Sciences, and Engineering and Applied Science. We request a multiphoton microscope equipped to perform three separate, but related efforts, in cellular programming. The efforts include: At the single cell level, where we use a novel technology developed by one of the project PIs (Jim Eberwine, Pharmacology, School of Medicine) to controllably deliver a define mRNA population to living cells to redirect their cellular phenotype, At the multicellular scale, where we use novel photopolymer formulations to assemble complex, three-dimensional cell culture substrates (Chris Chen, Bioengineering, School of Engineering and Applied Science) with tunable microenvironments for building vascularized tissue and cartilage, and At the tissue scale, where we use widely available optical activation techniques to study the in vivo programming of neural circuits in the cortex and hippocampus to understand changes that occur during disease or injury (David Meaney, Bioengineering, School of Engineering and Applied Science). This new microscope system will replace an existing 12 year old BioRad multiphoton microscope in the engineering complex at the University of Pennsylvania. The current BioRad system does meet the high technical demands of the above applications. Moreover, there is no widely available existing system on the Penn campus to perform this work. Therefore, there is substantial need for this microscope system. The combination of these three 'base technologies' on one microscope platform can significantly advance research topics in broadly diverse areas such as cellular differentiation, regenerative medicine, and the etiology of neurological disease and neurobehavior. The potential of integrating two or three of the base technologies into a single topic area provides nearly limitless possibilities for cutting edge advances in how living systems form and regenerate tissue, as well as developing a platform for assembling novel tissue replacement or RNA-based therapeutic approaches.

描述（由申请人提供）：这个关于定制多光子显微镜的共享仪器提案是宾夕法尼亚大学在细胞编程方面更大的机构努力的一部分。从战略上讲，这项工作位于宾夕法尼亚大学五所学院——医学、牙科、兽医、艺术与科学、工程与应用科学学院的交叉点。我们需要一台多光子显微镜，能够在细胞编程中执行三项独立但相关的工作。这些努力包括： 在单细胞水平上，我们使用项目 PI 之一（Jim Eberwine，医学院药理学）开发的一项新技术，将特定的 mRNA 群体可控地递送至活细胞，以重定向其细胞表型，在多细胞尺度上，我们使用新型光聚合物配方来组装复杂的三维细胞培养基质（工程与应用科学学院生物工程系的 Chris Chen），并具有可调节的微环境来构建血管化组织和软骨，以及在组织尺度上，我们使用广泛使用的光学激活技术来研究皮层和海马神经回路的体内编程，以了解疾病或损伤期间发生的变化（David Meaney，生物工程学院，工程和应用科学）。这个新的显微镜系统将取代宾夕法尼亚大学工程综合体中已有 12 年历史的 BioRad 多光子显微镜。目前的BioRad系统确实满足了上述应用的高技术要求。此外，宾夕法尼亚大学校园没有广泛可用的现有系统来执行这项工作。因此，非常需要这种显微镜系统。这三种“基础技术”在一个显微镜平台上的结合可以显着推进细胞分化、再生医学以及神经疾病和神经行为的病因学等广泛领域的研究课题。将两种或三种基础技术整合到一个主题领域的潜力为生命系统如何形成和再生组织的前沿进展提供了几乎无限的可能性，并开发了一个用于组装新型组织替代或基于 RNA 的治疗方法的平台。