Acquisition of a Multiphoton Microscope for Cellular Programming

获取用于细胞编程的多光子显微镜

• 批准号: 7793841
• 负责人: DAVID F MEANEY
• 金额: $ 50万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793841
• 关键词: 12 year old; Applied Research; Area; Arts; Biomedical Engineering; Cartilage; Cell Culture Techniques; Cells; Complex; Dental; Disease; Drug Formulations; Engineering; Etiology; Hippocampus (Brain); Injury; Life; Messenger RNA; Microscope; Natural regeneration; Optics; Organism; Pennsylvania; Pharmacology; Phenotype; Population; Regenerative Medicine; Research; Schools; Science; System; Techniques; Technology; Therapeutic; Tissues; Universities; Work; base; in vivo; instrumentation; medical schools; meetings; nervous system disorder; neural circuit; neurobehavior; new technology; novel; programs

DESCRIPTION (provided by applicant): This shared instrumentation proposal for a customized multiphoton microscope is part of a larger institutional effort at Penn in cellular programming. Strategically, this effort lies at the intersection between five of Penn's schools - Medicine, Dental, Veterinary, Arts and Sciences, and Engineering and Applied Science. We request a multiphoton microscope equipped to perform three separate, but related efforts, in cellular programming. The efforts include: At the single cell level, where we use a novel technology developed by one of the project PIs (Jim Eberwine, Pharmacology, School of Medicine) to controllably deliver a define mRNA population to living cells to redirect their cellular phenotype, At the multicellular scale, where we use novel photopolymer formulations to assemble complex, three-dimensional cell culture substrates (Chris Chen, Bioengineering, School of Engineering and Applied Science) with tunable microenvironments for building vascularized tissue and cartilage, and At the tissue scale, where we use widely available optical activation techniques to study the in vivo programming of neural circuits in the cortex and hippocampus to understand changes that occur during disease or injury (David Meaney, Bioengineering, School of Engineering and Applied Science). This new microscope system will replace an existing 12 year old BioRad multiphoton microscope in the engineering complex at the University of Pennsylvania. The current BioRad system does meet the high technical demands of the above applications. Moreover, there is no widely available existing system on the Penn campus to perform this work. Therefore, there is substantial need for this microscope system. The combination of these three 'base technologies' on one microscope platform can significantly advance research topics in broadly diverse areas such as cellular differentiation, regenerative medicine, and the etiology of neurological disease and neurobehavior. The potential of integrating two or three of the base technologies into a single topic area provides nearly limitless possibilities for cutting edge advances in how living systems form and regenerate tissue, as well as developing a platform for assembling novel tissue replacement or RNA-based therapeutic approaches.

描述（由申请人提供）：定制多光子显微镜的共享仪器提案是Penn在Cellular编程中较大机构努力的一部分。从战略上讲，这项工作在于宾夕法尼亚州五所学校之间的交集 - 医学，牙科，兽医，艺术和科学以及工程和应用科学。我们要求在蜂窝编程中进行三个单独但相关的工作，该显微镜可以执行三个单独的但相关的工作。 The efforts include: At the single cell level, where we use a novel technology developed by one of the project PIs (Jim Eberwine, Pharmacology, School of Medicine) to controllably deliver a define mRNA population to living cells to redirect their cellular phenotype, At the multicellular scale, where we use novel photopolymer formulations to assemble complex, three-dimensional cell culture substrates (Chris Chen, Bioengineering, School of Engineering and Applied Science)具有可调的微环境，用于建造血管化组织和软骨，并在组织尺度上使用，在那里我们使用广泛可用的光学激活技术来研究皮质和海马中神经回路的体内编程，以了解疾病或疾病期间发生的变化（David Meaney，Bioengineering，Bioengineering，Engineering，Engineering和Applied Science和Applied Science）。这个新的显微镜系统将取代宾夕法尼亚大学工程综合体中现有的12岁的Biorad多光子显微镜。当前的Biorad系统确实满足了上述应用程序的高技术需求。此外，宾夕法尼亚州校园没有可用的现有系统来执行这项工作。因此，该显微镜系统非常需要。在一个显微镜平台上，这三种“基础技术”的组合可以显着推进各种不同领域的研究主题，例如细胞分化，再生医学以及神经系统疾病和神经行为的病因。将两个或三种基础技术纳入单个主题领域的潜力为生命系统如何形成和再生组织的前沿进步提供了几乎无限的可能性，并开发了组装新型的组织更换或基于RNA的治疗方法的平台。