Acquisition of a Leica TCS LSI Zoom Confocal Microscope

购买 Leica TCS LSI 变焦共焦显微镜

• 批准号: 7791241
• 负责人: Laura S Van Winkle
• 金额: $ 28.65万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7791241
• 关键词: Agriculture; Air Pollutants; Animal Model; Animals; Asthma; Autistic Disorder; Basic Science; Brain; Cardiovascular system; Cells; Childhood; Communities; Complex; Computer software; Core Facility; Disease; Ecology; Educational process of instructing; Elements; Engineering; Equipment; Fishes; Functional disorder; Health; Human; Image; Inhalation Toxicology; Investments; Life; Medicine; Microscope; Neurons; Postdoctoral Fellow; Research; Research Personnel; Resolution; Schedule; Schools; Specimen; Stem cells; System; Time; Tissues; Vascular Diseases; Veterinary Medicine; Work; college; flexibility; graduate student; instrument; interest; outreach; programs

DESCRIPTION (provided by applicant): The instrument requested is a Leica TCS LSI zoom confocal microscope system. We are requesting the current configuration because it has the greatest flexibility in capability and can capture images from live tissues and cells as well as image very large fields of view. The other confocal systems currently available on campus are not sufficient for the projects described because they 1) are too slow, 2) cannot image large fields of view without stitching, 3) are highly scheduled already, 4) are not the correct configuration for our user needs being primarily inverted micrscopes with small working distances and 5) do not have an environmental chamber for imaging large live specimens. There is not currently available on this campus any truly comparable confocal microscope in terms of capability. Hence this microscope will enable studies that were not previously possible or were only achievable in a substandard way with a tremendous investment of both money and time. The Leica TCS LSI differs significantly from other confocals primarily in the ability to image large fields of view and to zoom from low to high magnification. The software is easy to use and the image capture is high resolution and very fast; a major advantage for a multi-user piece of equipment requiring high throughput. The new equipment is needed to advance the research programs of 14 investigators on the UC Davis campus. The users represent a broad range of scientific interest and span four schools/colleges on campus (Medicine, Veterinary Medicine, Agriculture and Environmental Sciences and Engineering). The research is highly focused on both basic sciences and human health and includes studies of inhalation toxicology of air pollutants, animal models of asthma, studies of the circulatory system including heart/vascular disease, brain and nervous system dysfunction, stem cell repopulation of airways and whole animal studies of medaka fish. All the proposals have one common element, the need for a microscope with a large field of view and high resolution to analyze complex composites of cells either in culture or in tissues, quickly. Placement of this equipment in core facilities at UC Davis will also facilitate the projects of graduate students, postdoctoral fellows, residents and professional school (DVM or MD) researchers. Outreach to other campus users will be facilitated by maintaining the equipment in a core facility, listing the equipment on the core and center websites and by inclusion in Dr. Van Winkle's teaching in campus courses on imaging. The overall benefits to the UC Davis campus are many. This equipment fits well with the research needs of the UC Davis community, which has included a recent emphasis on the childhood health disorders autism and asthma that require large field imaging capability for neurons and airways respectively.

描述（由申请人提供）：请求的仪器是Leica TCS LSI Zoom共聚焦显微镜系统。我们要求当前的配置，因为它的功能具有最大的灵活性，并且可以捕获来自活组织和单元格的图像以及图像非常大的视野。目前校园中目前可用的其他共同系统对于所描述的项目不足，因为它们1）太慢，2）不能在没有缝线的情况下映像大型视野，3）已经高度安排了，4）不是正确的配置，因为我们的用户需求主要是倒置的微观距离，而较小的工作距离和5）没有一个环境室，可用于成像大型现场表明。目前，在该校园里，就能力而言，任何真正可比的共聚焦显微镜都没有可用。因此，该显微镜将实现以前无法实现的研究，或者只能以不合标准的方式获得金钱和时间的巨大投资。 Leica TCS LSI与其他共同焦点显着不同，主要是在图像较大的视野和从低放大倍率上放大的能力。该软件易于使用，图像捕获是高分辨率且非常快的。需要高通量的多用户设备的主要优势。需要新设备来推进加州大学戴维斯分校校园14位调查人员的研究计划。用户代表着广泛的科学兴趣，并在校园（医学，兽医医学，农业和环境科学与工程学）上跨越了四所学校/学院。这项研究高度关注基础科学和人类健康，包括对空气污染物的吸入毒理学，哮喘动物模型，循环系统的研究，包括心脏/血管疾病，脑/血管疾病，脑和神经系统功能障碍，干细胞再生的循环系统研究，对甲麦加鱼类的整个动物研究。所有建议都有一个共同的元素，需要具有较大视野的显微镜和高分辨率来分析培养物或组织中细胞的复杂复合材料，迅速。将该设备放置在加州大学戴维斯分校的核心设施中，还将促进研究生，博士后研究员，居民和专业学校（DVM或MD）研究人员的项目。通过将设备维护在核心设施中，在核心和中心网站上列出设备，并纳入Van Winkle博士在Imaging校园课程中的教学，可以促进与其他校园用户的宣传。加州大学戴维斯分校校园的总体好处很多。该设备非常符合加州大学戴维斯分校社区的研究需求，该设备最近重点介绍了儿童健康疾病的自闭症和哮喘，分别需要对神经元和气道的大型现场成像能力。