Acquisition of LMD6000 Laser Microdissection Unit

收购 LMD6000 激光显微切割装置

• 批准号: 7387980
• 负责人: Laura S Van Winkle
• 金额: $ 24.37万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7387980
• 关键词: Agriculture; Air Pollutants; Animal Model; Area; Asthma; Basic Science; Cells; Communities; Complex; Core Facility; Dioxins; Dissection; Ecology; Elements; Embryo; Environmental Health; Equipment; Fertility; Funding Agency; Genetic; Genomics; Health; Heart Diseases; Human; Individual; Inflammation; Inhalation Toxicology; Kidney; Lasers; Life; Lung; Microdissection; Microscope; Neoplasm Metastasis; Pancreas; Pattern; Plants; Proteomics; Range; Reproduction; Research; Research Personnel; Sampling; Schedule; Seeds; Seminiferous tubule structure; Speed; Stem cells; System; Tissues; Toxic effect; Toxicology; Veterinary Medicine; Veterinary Schools; bone; college; instrument; interest; keratinocyte; laser capture microdissection; malignant breast neoplasm; medical schools; member; outreach; programs; repaired

DESCRIPTION (provided by applicant): The instrument requested is a Leica LMD6000 Multi-user Laser Capture Microdissection Microscope System. We are requesting the multi-user configuration of this equipment because it has the capability to capture tissues and cells for proteomics, genetics, and live cell subcloning; features that are required by our user group. The other laser capture systems currently available on campus are not sufficient for the projects described because they 1) are too slow, 2) cannot do large areas of tissue quickly, 3) require expensive technical support and are highly scheduled already, 4) cannot cut hard tissues such as plants, embryos or decalcified bone. The Leica LMD6000 differs significantly from other laser capture units primarily in the mechanism of sample capture as well as speed and ease of use; a major advantage for a multi-user piece of equipment requiring high throughput. The new equipment is needed to advance the research programs of 15 investigators on the UC Davis campus. Many of these investigators are already collaborators; 11 are members of an NIEHS Center for Environmental Health Sciences and 5 are co-investigators on an NIEHS Program project, in addition to their individual funding sources. The users represent a broad range of scientific interest on campus in College of Biosciences (2), School of Medicine (4), School of Veterinary Medicine (6), and College of Agriculture and Environmental Sciences (3). The research is highly focused on both basic sciences and human health and includes studies of inhalation toxicology of air pollutants, animal models of asthma, stem cell repair in the lung, cell fate patterning in plant and mammalian embryos and in seminiferous tubules, heart disease, fertility and reproduction, general toxicology, inflammation, breast cancer metastases, dioxin effects on pancreas and kidney and keratinocyte function/toxicity. All the proposals have one common element, the need for laser dissection to quickly analyze complex composites of cells either in culture or in tissues. Outreach to other campus users will be facilitated by maintaining the equipment in a core facility, listing on the CEHS Center's website, and by mentioning the equipment in Dr. Van Winkle's graduate course. The overall benefits to the UC Davis campus are many. This equipment fits well with the research needs of the UC Davis community, which has included a recent emphasis on genomics.

描述（由申请人提供）：请求的仪器是Leica LMD6000多用户激光捕获显微镜显微镜系统。我们要求该设备的多用户配置，因为它具有捕获蛋白质组学，遗传学和活细胞亚克隆的组织和细胞的能力。我们的用户组所需的功能。目前校园中目前可用的其他激光捕获系统对于所描述的项目不足，因为它们1）太慢，2）不能快速进行大面积的组织，3）需要昂贵的技术支持并且已经高度安排了，4）无法切割硬组织，例如植物，胚胎，胚胎或脱落的骨头。 Leica LMD6000与其他激光捕获单元显着不同，主要是在样品捕获机理以及速度和易用性方面。需要高通量的多用户设备的主要优势。需要新设备来推进加州大学戴维斯分校校园15名调查人员的研究计划。这些调查人员中有许多已经是合作者。 11是NIEHS环境健康科学中心的成员，除了个人资金来源外，还有5个是NIEHS计划项目的共同评估者。用户代表了生物科学学院（2），医学院（4），兽医学院（6）和农业与环境科学学院（3）的校园的广泛科学兴趣。 The research is highly focused on both basic sciences and human health and includes studies of inhalation toxicology of air pollutants, animal models of asthma, stem cell repair in the lung, cell fate patterning in plant and mammalian embryos and in seminiferous tubules, heart disease, fertility and reproduction, general toxicology, inflammation, breast cancer metastases, dioxin effects on pancreas and kidney and角质形成症功能/毒性。所有建议都有一个共同的元素，需要激光解剖来快速分析培养物或组织中细胞的复杂复合材料。通过将设备维护在CEHS中心的网站上，并在Van Winkle博士的研究生课程中提及设备，可以通过将设备维护在核心设施中，从而促进与其他校园用户的宣传。加州大学戴维斯分校校园的总体好处很多。该设备非常适合加州大学戴维斯分校社区的研究需求，该社区最近重点是基因组学。