In-Incubator Live Cell Fluorescence/DIC Time Lapse Imaging System

培养箱内活细胞荧光/DIC 延时成像系统

• 批准号: 7792006
• 负责人: MARTIN F SCHNEIDER
• 金额: $ 14.74万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7792006
• 关键词: Address; Adult; Arts; Award; Baltimore; Biochemistry; Biological; Biomedical Research; Breast Cancer Cell; Cell Culture Techniques; Cell Shape; Cell physiology; Cells; Chimeric Proteins; Collaborations; DNA Repair; Development; Epithelial Cells; Event; Exposure to; Fees; Fluorescence; Funding; Gases; Goals; Grant; Hour; Image; Imaging technology; Incubators; Learning; Life; Maintenance; Maryland; Microscope; Mission; Molecular; Molecular Biology; Monitor; Morphology; Muscle Fibers; Neurons; Optics; Principal Investigator; Research; Research Personnel; Signaling Molecule; Stratification; Students; Subcellular structure; Surface; System; Temperature; Time; Tissues; Training; United States National Institutes of Health; Universities; cell behavior; design; extracellular; human disease; instrument; instrumentation; medical schools; movie; research facility; skeletal; tissue culture; uptake

DESCRIPTION (provided by applicant): Principal investigators at the University of Maryland, Baltimore (UMB) have a pressing need for an Olympus Viva-View In-Incubator Microscope System to monitor long-term changes in cell and tissue function and morphology. This system will support twelve NIH R01 grants awarded to six investigators at the University of Maryland SOM, as well as other grants. Because the microscope is designed inside an incubator and includes port for media change, cell cultures can be viewed over long periods under constant conditions of temperature and gas. The microscope uses DIC or epi-fluorescence optics to acquire images, movies, z- stacks, etc. This capability is ideal for monitoring changes in cell shape, intracellular structure, and intracellular distribution of fusion proteins, and intracellular distribution of fluorescent indicators (e.g., indo-1 dual emission Ca2+ imaging) at times ranging from hours to weeks. To promote scientific exchange, stimulate research collaborations, and increase user availability, the in-incubator microscope system will be incorporated into the existing Biochemistry and Molecular Biology Instrumentation Core (BMBIC) located in the Biomedical Research Facility (BRF). The BRF is located in a remote corner of campus and the BMBIC was developed to support investigator instrument needs in this quadrant of campus. Recent renovation of BMBIC space indicates the commitment of the University of Maryland School of Medicine and the Department of Biochemistry and Molecular Biology to this facility. A key long range research goal of the all major users to understand long-term biological events at the molecular level as it pertains to human disease. The new in- incubator microscope will make this potential available. We believe it is important for students and postdoctoral associates at UMB to gain exposure to a wide range of imaging technology and to learn how to monitor long-term changes in cell function. Indeed, providing access to state-of-the-art instrumentation is a key aspect of the training mission at UMB. The new in-incubator microscope will enhance the training of students supported by at least two NIH-funded training grants (T32GM066706-05 and 5T32AR007592-13). This system will enable research to address key questions relating to events regulating dedifferentiation of cultured adult skeletal muscle fibers, skeletal myotube and myofiber development and formation, surface epithelial cell stratification and differentiation, breast cancer cell behavior, DNA damage repair and uptake and intracellular redistribution of extracellular signaling molecules in neurons. A tissue culture hood and standard tissue culture incubator are available in the BMBIC to support this instrument. General oversight for the BMBIC is provided by the BMBIC Oversight Committee and a specific oversight group is assigned to this instrument. Instrument maintenance is supported by the School of Medicine, the Department of Biochemistry and Molecular Biology, and users fees.

描述（由申请人提供）：巴尔的摩大学马里兰州（UMB）的主要研究人员迫切需要Olympus Viva-View-View Inucubator显微镜系统，以监视细胞功能和组织功能和形态的长期变化。该系统将支持马里兰州SOM的六名调查员以及其他赠款的十二项NIH R01赠款。由于显微镜是在孵化器内设计的，并包括用于培养基变化的端口，因此可以在温度和气体的恒定条件下长时间观察细胞培养物。显微镜使用DIC或Epi-fluorescent Optics获取图像，电影，Z堆栈等。此能力是监测融合蛋白的细胞形状，细胞内结构和细胞内分布的变化的理想性，以及荧光指示剂的细胞内分布（例如，Indo-1 Dial Soughtion Ca2+ Dial Soughtion Ca2+ Tiles tiles tirs tirs the Weeks dekessing dekess theeks geweech ty Bekess geweece。为了促进科学交流，刺激研究合作并提高用户的可用性，内存显微镜系统将纳入位于生物医学研究机构（BRF）的现有生物化学和分子生物学仪器核心（BMBIC）。 BRF位于校园的一个偏远角落，开发了BMBIC，以支持校园象限的研究人员的需求。 BMBIC空间的最新翻新表明，马里兰大学医学院以及生物化学和分子生物学系对此设施的承诺。所有主要用户的关键远程研究目标是与人类疾病有关的分子水平上的长期生物事件。新的孵化器显微镜将使这种潜力可用。我们认为，对于UMB的学生和博士后同伴来说，重要的是要获得广泛的成像技术，并学习如何监测细胞功能的长期变化。确实，提供最先进的仪器是UMB培训任务的关键方面。新的调查器显微镜将增强至少两项由NIH资助的培训补助金（T32GM066706-05和5T32AR007592-13）支持的学生的培训。 This system will enable research to address key questions relating to events regulating dedifferentiation of cultured adult skeletal muscle fibers, skeletal myotube and myofiber development and formation, surface epithelial cell stratification and differentiation, breast cancer cell behavior, DNA damage repair and uptake and intracellular redistribution of extracellular signaling molecules in neurons. BMBIC可以提供组织培养引擎盖和标准组织培养孵化器，以支持该仪器。 BMBIC监督委员会提供了对BMBIC的一般监督，并将特定的监督小组分配给了该工具。仪器维护得到医学院，生物化学和分子生物学系以及用户费用的支持。