Glycomics Equipment: NanoLC CHIP-QTOF Mass Spectrometer

糖组学设备：NanoLC CHIP-QTOF 质谱仪

• 批准号: 7794794
• 负责人: CARLITO B LEBRILLA
• 金额: $ 50万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794794
• 关键词: Biological; Biological Markers; Cells; Chemistry; Complex; Devices; Disease Marker; Early Diagnosis; Enzymes; Equipment; Health; Helicobacter pylori; Human; Infection; Laboratories; Liquid Chromatography; Liquid substance; Mass Spectrum Analysis; Molecular; Mucins; Necrotizing Enterocolitis; Oligosaccharides; Physics; Polysaccharides; Principal Investigator; Probiotics; Reaction; Research; Serum; Site; Stomach; Structure; Time; United States National Institutes of Health; analytical tool; cancer diagnosis; design; disease diagnosis; instrument; malignant stomach neoplasm; mass analyzer; mass spectrometer; medical schools; microchip; prebiotics; public health relevance

DESCRIPTION (provided by applicant): The proposed instrument will be the central analytical tool for the glycomics profiling in several NIH projects focused on infection and disease diagnosis. The instrument includes an integrated nanoflow liquid chromatography device with a quadrupole time-of-flight (Q-TOF) mass analyzer. The instrument is similar to an existing instrument that is on loan in the Principal Investigator's laboratory. The new instrument will include tandem MS capability for online structural analysis. The proposed instrument will be used to analyze oligosaccharides and determine their structures in highly complicated biological mixtures. Projects that the instrument will be used for include: (1) The determination of O-glycans in stomach mucin to determine the site of interaction with Helicobacter pylori; Serum glycan biomarkers for early detection of H. pylori-associated gastric cancer (Dr. Jay Solnick, School of Medicine), (2) The effect of prebiotics and probiotics in necrotizing enterocolitis (Dr. Mark Underwood, School of Medicine), (3) The profiling of oligosaccharides by nanoLC Chip/Q-TOF mass spectrometry (Dr. Carlito Lebrilla, Department of Chemistry and School of Medicine), (4) Mass spectrometry identification of molecular complexes in cell lysates and "hit compounds" (Dr. Xiangdong Zhu, Department of Physics), (5) The search of glycans markers for disease (Dr. Kit Lam, School of Medicine), (6) The analysis of products and intermediates in multiple-enzyme reactions (Dr. Xi Chen, Department of Chemistry), and (7) The design and synthesis of chemotherapeutics (Dr. Jacqueline Gervay-Hague, Department of Chemistry). PUBLIC HEALTH RELEVANCE: A nanoflow liquid chromatography time-of-flight mass spectrometry instrument with an integrated microchip separation device is requested for performing glycomics profiling of biological fluids. The instrument will be used for research related to human health in terms of gut health, cancer diagnosis, and infection.

描述（由申请人提供）：拟议的仪器将成为 NIH 几个专注于感染和疾病诊断的项目中糖组学分析的核心分析工具。该仪器包括一个集成纳流液相色谱装置和四极杆飞行时间 (Q-TOF) 质量分析器。该仪器与首席研究员实验室借用的现有仪器类似。新仪器将包括用于在线结构分析的串联质谱功能。拟议的仪器将用于分析低聚糖并确定其在高度复杂的生物混合物中的结构。该仪器将用于的项目包括：（1）测定胃粘蛋白中的O-聚糖，以确定与幽门螺杆菌相互作用的位点；用于早期检测幽门螺杆菌相关胃癌的血清聚糖生物标志物（Jay Solnick 博士，医学院），(2) 益生元和益生菌在坏死性小肠结肠炎中的作用（Mark Underwood 博士，医学院），(3 ) 通过 nanoLC 芯片/Q-TOF 质谱分析寡糖（Carlito Lebrilla 博士，化学系和医学院），(4) Mass细胞裂解物和“命中化合物”中分子复合物的光谱鉴定（物理系朱向东博士），（5）疾病聚糖标记物的搜索（医学院林洁特博士），（6）分析多酶反应中的产物和中间体的合成（陈曦博士，化学系），以及（7）化疗药物的设计和合成（Jacqueline Gervay-Hague 博士，化学系）化学）。 公共健康相关性：需要具有集成微芯片分离装置的纳流液相色谱飞行时间质谱仪来对生物液体进行糖组学分析。该仪器将用于肠道健康、癌症诊断和感染等与人类健康相关的研究。